Militarine 

Militarine is a plant growth inhibitor and anti-inflammatory agent. Militarine inhibits the elongation of radicles and hypocotyls in seedlings of lettuce, Italian ryegrass and timothy grass. Militarine alleviates PM_2.5-induced inflammatory injury and inhibits cell migration in human alveolar epithelial A549 cells by inhibiting the NF-κB signaling pathway, reducing oxidative stress and the release of inflammatory factors. Militarine can be used in studies related to PM_2.5-induced pulmonary diseases^[1][2].

Militarine (52 h) inhibits the elongation of radicles and hypocotyls in seedlings of lettuce (Lactuca sativa L. cv. Great Lakes 366), with an EC₅₀ value of 0.28 mM for radicles and 1.03 mM for hypocotyls^[1].
Militarine (52 h) inhibits the elongation of radicles and hypocotyls in seedlings of Lolium multiflorum Lam., with an EC₅₀ value of 0.43 mM for radicles and 1.18 mM for hypocotyls^[1].
Militarine (58 h) inhibits the elongation of radicles and hypocotyls in Phleum pratense L. seedlings, with corresponding EC₅₀ values of 0.32 mM and 0.82 mM for radicles and hypocotyls, respectively^[1].
Militarine (1.25-60 μg/mL; 24 h) reduces the viability of A549 cells^[2].
Militarine (1.25-10 μg/mL; 20 h) reduces the secretion levels of IL-6 and TNF-α in A549 cells exposed to PM_2.5^[2].
Militarine (1.25-10 μg/mL; 20 h) inhibits the mRNA expression of IL-6, TNF-α, IL-1β and COX-2, and reduces the accumulation level of ROS in PM_2.5-exposed A549 cells^[2].
Militarine (1.25-10 μg/mL; 20 h) reduces the apoptosis level of A549 cells after PM_2.5 exposure, and inhibits cell migration and invasion^[2].
Militarine (1.25-10 μg/mL; 20 h) inhibits the activation of the NF-κB pathway by reducing the phosphorylation levels of IKKβ, IκBα and NF-κBp65 in A549 cells exposed to PM_2.5; in addition, 5 and 10 μg/mL Militarine reduces the COX-2 protein level in PM_2.5-exposed cells after 18 h of exposure^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

726.72

C₃₄H₄₆O₁₇

58139-23-4

Solid

White to light yellow

O[C@H]([C@H]1O)[C@@H](O[C@@H]([C@H]1O)CO)OC(C=C2)=CC=C2COC([C@](CC(C)C)(O)CC(OCC(C=C3)=CC=C3O[C@@H]([C@@H]([C@H]4O)O)O[C@@H]([C@H]4O)CO)=O)=O

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Sakuno E, et al. Contribution of militarine and dactylorhin A to the plant growth‐inhibitory activity of a weed‐suppressing orchid, Bletilla striata. Weed biology and management, 2010, 10(3): 202-207.

  [2]. Cheng W, et al. Inhibition of inflammation-induced injury and cell migration by coelonin and militarine in PM_2.5-exposed human lung alveolar epithelial A549 cells. Eur J Pharmacol. 2021;896:173931.  [Content Brief]