2. Cell Cycle/DNA Damage PI3K/Akt/mTOR
  3. ATM/ATR
  4. Mirin

Mirin is a potent Mre11-Rad50-Nbs1 (MRN) complex inhibitor. Mirin prevents MRN-dependent activation of ATM (IC50=12 μM) without affecting ATM protein kinase activity, and it inhibits Mre11-associated exonuclease activity. Mirin abolishes the G2/M checkpoint and homology-dependent repair in mammalian cells. Mirin prevents ATM activation in response to DNA double-strand breaks (DSBs) and blocks homology-directed repair (HDR) in mammalian cells.

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Mirin Chemical Structure

Mirin Chemical Structure

CAS No. : 299953-00-7

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Based on 1 publication(s) in Google Scholar

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Mirin Related Antibodies

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1 Publications Citing Use of MCE Mirin

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ATM ATR

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Description

Mirin is a potent Mre11-Rad50-Nbs1 (MRN) complex inhibitor. Mirin prevents MRN-dependent activation of ATM (IC50=12 μM) without affecting ATM protein kinase activity, and it inhibits Mre11-associated exonuclease activity. Mirin abolishes the G2/M checkpoint and homology-dependent repair in mammalian cells. Mirin prevents ATM activation in response to DNA double-strand breaks (DSBs) and blocks homology-directed repair (HDR) in mammalian cells[1].

In Vitro

Mirin inhibits H2AX phosphorylation with an IC50 of 66 μM. Mirin also inhibits the ATM-dependent phosphorylation of the downstream targets Nbs1 and Chk2 and the MRN-dependent autophosphorylation of ATM at Ser1981 in response to DSBs. Mirin induces a substantial G2 arrest at concentrations of 50 μM and 100 μM. Mirin (10-100 μM) inhibits homology-dependent DNA repair in TOSA4 cells[1].
BRCA2-deficient cells also showed hypersensitivity to the Mre11 inhibitor Mirin[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mirin Related Antibodies
Molecular Weight

220.25

Formula

C10H8N2O2S
CAS No.
Appearance

Solid

Color

Brown to orange

SMILES

O=C1N=C(N)S/C1=C/C2=CC=C(O)C=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (378.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.5403 mL 22.7015 mL 45.4030 mL
5 mM 0.9081 mL 4.5403 mL 9.0806 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.5403 mL 22.7015 mL 45.4030 mL 113.5074 mL
5 mM 0.9081 mL 4.5403 mL 9.0806 mL 22.7015 mL
10 mM 0.4540 mL 2.2701 mL 4.5403 mL 11.3507 mL
15 mM 0.3027 mL 1.5134 mL 3.0269 mL 7.5672 mL
20 mM 0.2270 mL 1.1351 mL 2.2701 mL 5.6754 mL
25 mM 0.1816 mL 0.9081 mL 1.8161 mL 4.5403 mL
30 mM 0.1513 mL 0.7567 mL 1.5134 mL 3.7836 mL
40 mM 0.1135 mL 0.5675 mL 1.1351 mL 2.8377 mL
50 mM 0.0908 mL 0.4540 mL 0.9081 mL 2.2701 mL
60 mM 0.0757 mL 0.3784 mL 0.7567 mL 1.8918 mL
80 mM 0.0568 mL 0.2838 mL 0.5675 mL 1.4188 mL
100 mM 0.0454 mL 0.2270 mL 0.4540 mL 1.1351 mL
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Mirin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Mirin299953-00-7ATM/ATRAtaxia telangiectasia mutatedATM and RAD3 relatedH2AXphosphorylationChk2G2arrestDSBrepairInhibitorinhibitorinhibit

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