2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. Mitonafide

Mitonafide  (Synonyms: NSC 300288)

Cat. No.: HY-119182 Purity: 99.79%
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Mitonafide (NSC 300288) is a cytostatic agent. Mitonafide binds to double-stranded DNA through intercalation, and inhibits DNA and RNA synthesis. Mitonafide is an antitumor agent that can be used in the research of cancers, such as non-small cell lung cancer (NSCLC), leukemia.

For research use only. We do not sell to patients.

Mitonafide Chemical Structure

Mitonafide Chemical Structure

CAS No. : 54824-17-8

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Based on 1 publication(s) in Google Scholar

Mitonafide Related Antibodies

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Description

Mitonafide (NSC 300288) is a cytostatic agent. Mitonafide binds to double-stranded DNA through intercalation, and inhibits DNA and RNA synthesis. Mitonafide is an antitumor agent that can be used in the research of cancers, such as non-small cell lung cancer (NSCLC), leukemia[1][2][3].

IC50 & Target

DNA and RNA synthesis[1]
In Vitro

Mitonafide inhibits DNA and RNA synthesis and induces single-strand breaks in the DNA of chinese hamster ovary cells[1].
The incubation of Mitonafide with rat liver microsomes and NADPH under anaerobic conditions results in the formation of a metabolite identified as 5-aminomitonafide[2].
Mitonafide (25, 50 μM, 1 h) induces single-stand breaks in the DNA of L1210 cells[2].
Mitonafide (10-100 μM) exhibits cytotoxic effect in the HOP-62 lung cell line[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mitonafide Related Antibodies

Cell Viability Assay[6]

Cell Line: SK-OV-3, HepG2, A-549, T-24, SMMC-7721, HL-7702
Concentration: 0-100 μM respectively.
Incubation Time: 48 h
Result: Inhibited cell viability with IC50 values of 6.26, 10.88, 7.94, 5.01, 6.94, 8.51 μM.
In Vivo

Mitonafide (0.5 and 1 mg/kg, i.p., 1-7 days) shows antitumoral potency in S-180 bearing mice[4].
Mitonafide (5 mg/kg, i.p., twice a day) shows anticancer activity in HepG2 xenograft model[6].
Mitonafide (single i.p. injection, S-180 bearing mice) shows the LD50 value of 10.0 mg/kg[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: S-180 bearing mice[4]
Dosage: 0.5 mg/kg and 1 mg/kg
Administration: Intraperitoneal injection (i.p.) for 1-7 days
Result: Increased in median survival times.
Animal Model: HepG2 xenograft model [6]
Dosage: 5 mg/kg
Administration: Intraperitoneal injection (i.p.), twice a day.
Result: Exhibited a relative tumor increment rates (T/C) value of 28.8%.
Molecular Weight

313.31

Formula

C16H15N3O4
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1N(C(C2=CC([N+]([O-])=O)=CC3=CC=CC1=C23)=O)CCN(C)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (199.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1917 mL 15.9586 mL 31.9173 mL
5 mM 0.6383 mL 3.1917 mL 6.3835 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1917 mL 15.9586 mL 31.9173 mL 79.7932 mL
5 mM 0.6383 mL 3.1917 mL 6.3835 mL 15.9586 mL
10 mM 0.3192 mL 1.5959 mL 3.1917 mL 7.9793 mL
15 mM 0.2128 mL 1.0639 mL 2.1278 mL 5.3195 mL
20 mM 0.1596 mL 0.7979 mL 1.5959 mL 3.9897 mL
25 mM 0.1277 mL 0.6383 mL 1.2767 mL 3.1917 mL
30 mM 0.1064 mL 0.5320 mL 1.0639 mL 2.6598 mL
40 mM 0.0798 mL 0.3990 mL 0.7979 mL 1.9948 mL
50 mM 0.0638 mL 0.3192 mL 0.6383 mL 1.5959 mL
60 mM 0.0532 mL 0.2660 mL 0.5320 mL 1.3299 mL
80 mM 0.0399 mL 0.1995 mL 0.3990 mL 0.9974 mL
100 mM 0.0319 mL 0.1596 mL 0.3192 mL 0.7979 mL
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Mitonafide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Mitonafide54824-17-8NSC 300288NSC300288NSC-300288DNA/RNA SynthesisL1210single-strand breaksDNA5-aminomitonafideleukemiaantineoplasticantitumorS-180Inhibitorinhibitorinhibit

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