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  4. Myriocin

Myriocin  (Synonyms: Thermozymocidin; ISP-I)

Cat. No.: HY-N6798 Purity: 99.80%
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Myriocin (Thermozymocidin), a fungal metabolite could be isolated from Myriococcum albomyces, Isaria sinclairi and Mycelia sterilia, is a potent inhibitor of serine-palmitoyl-transferase (SPT) and a key enzyme in de novo synthesis of sphingolipids. Myriocin suppresses replication of both the subgenomic HCV-1b replicon and the JFH-1 strain of genotype 2a infectious HCV, with an IC50 of 3.5 μg/mL for inhibiting HCV infection.

For research use only. We do not sell to patients.

CAS No. : 35891-70-4

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Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of Myriocin:

Myriocin Related Antibodies

Top Publications Citing Use of Products

    Myriocin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(16):e2414260.  [Abstract]

    Western blots of autophagy flux from control or Arf1-deficient N2a cells treated with DMSO or Myriocin.

    Myriocin purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Feb 7:107648.  [Abstract]

    TRPC5 immunoreactivity associates with lipid raft fractions in TRPC5-overexpressing HEK293 cells following treatment with Myriocin (10 μM), Ly93 (1 μM), and PDMP (20 μM).

    Myriocin purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Feb 7:107648.  [Abstract]

    Average Ca2+ amplitude change evoked by EA in control conditions, and after treatment with Myriocin (10 μM), Ly93 (1 μM), or PDMP (20 μM).

    Myriocin purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 May 5;224(5):e202405060.  [Abstract]

    Western blot of p-IRE1α, XBP1s, p-PERK, and BiP in AML12 treated with Ctrl/CM ± inhibitors. The diamond indicates the nonspecific band. Inhibitors are FB1 (10 μM), Myriocin (10 μM), GW4869 (20 μM), and DPA (50 μM).

    Myriocin purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2022 Oct 8;12(1):169.  [Abstract]

    The cellular levels of ceramide in WT and CD151 overexpression HOS-MNNG cells with or without Myriocin treatment were analyzed by BODIPY FL-labeled ceramide confocal imaging; The cellular levels of lipid rafts in WT and CD151 overexpression HOS-MNNG cells with or without Myriocin treatment were analyzed by Alexa 555-conjugated CTB confocal imaging.

    Myriocin purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2022 Oct 8;12(1):169.  [Abstract]

    Representative images of clonogenic growth in CD151 overexpression cells treated with Myriocin.

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    Description

    Myriocin (Thermozymocidin), a fungal metabolite could be isolated from Myriococcum albomyces, Isaria sinclairi and Mycelia sterilia, is a potent inhibitor of serine-palmitoyl-transferase (SPT) and a key enzyme in de novo synthesis of sphingolipids. Myriocin suppresses replication of both the subgenomic HCV-1b replicon and the JFH-1 strain of genotype 2a infectious HCV, with an IC50 of 3.5 μg/mL for inhibiting HCV infection[1][2][3].

    IC50 & Target

    Serine-palmitoyl-transferase (SPT)[1]
    Cellular Effect
    Cell Line Type Value Description References
    MCF7 EC50
    0.0495 μM
    Compound: Myriocin
    Inhibition of SPT1 in human MCF7 cells assessed as suppression of 14C-serine incorporation into ceramide incubated for 2 hrs in presence of 4-HPR
    Inhibition of SPT1 in human MCF7 cells assessed as suppression of 14C-serine incorporation into ceramide incubated for 2 hrs in presence of 4-HPR
    		[PMID: 27213958]
    T-cell IC50
    3 nM
    Compound: 2, ISP-1
    Immunosuppressive activity in BALB/c/C57BL/6 mouse T cells assessed as inhibition of alloantigen-induced cell proliferation after 96 hrs by measuring [3H]thymidine uptake by mixed lymphocyte reaction assay
    Immunosuppressive activity in BALB/c/C57BL/6 mouse T cells assessed as inhibition of alloantigen-induced cell proliferation after 96 hrs by measuring [3H]thymidine uptake by mixed lymphocyte reaction assay
    		[PMID: 21456524]
    In Vitro

    Myriocin (Thermozymocidin; 0-10000 nM; 96 h; Huh7/Rep-Feo cells) inhibits the replication of a subgenomic hepatitis C virus (HCV) genotype 1b replicon[2].
    Myriocin (100 nM; 72 h) decreases in the sphingomyelin content of Huh7 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Myriocin Related Antibodies

    Western Blot Analysis[2]

    Cell Line: Huh7/Rep-Feo cells
    Concentration: 0, 300, and 1000 nM
    Incubation Time: 96 hours
    Result: Decreased the levels of HCV NS5A in a concentration-dependent manner.
    In Vivo

    Myriocin (Thermozymocidin; 0-1 mg/kg; i.p.; daily, for 5 d; male Balb/c mice) blocks de novo synthesis of sphingolipids in vivo by SPT inhibition and induced c-myc expression in liver[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Balb/c mice (22 g, 7-8 weeks old)[1]
    Dosage: 0, 0.1, 0.3, and 1.0 mg/kg
    Administration: Intraperitoneal injection; daily, for 5 days
    Result: Altered not significantly the levels of plasma alanine aminotransferase and aspartate aminotransferase.
    Decreased free sphinganine in a dose-dependent manner in both the liver and kidney.
    Decreased in the activity of liver SPT with a correlation coefficient of -0.76.
    Increased the expression of liver c-myc in a dose-dependent manner.
    Molecular Weight

    401.54

    Formula

    C21H39NO6
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CCCCCCC(CCCCCC/C=C/C[C@@H](O)[C@H](O)[C@](CO)(N)C(O)=O)=O
    Structure Classification
    Initial Source

    Myriococcum albomyces, Isaria sinclairi, Mycelia sterilia
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 2 mg/mL (4.98 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4904 mL 12.4521 mL 24.9041 mL
    5 mM --- --- ---
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 0.29 mg/mL (0.72 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.29 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (2.9 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 0.29 mg/mL (0.72 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.29 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (2.9 mg/mL) to 900 μL Corn oil, and mix evenly.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 5 mg/mL (12.45 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.80%

    SDS (479 KB)

    COA (241 KB) HNMR (276 KB) RP-HPLC (245 KB) LCMS (321 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.4904 mL 12.4521 mL 24.9041 mL 62.2603 mL
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    Myriocin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Myriocin35891-70-4Thermozymocidin ISP-IHCVAntibioticHepatitis C virusMyriococcum albomycesIsaria sinclairiMycelia steriliaserine-palmitoyl-transferasefungal metabolitesphingomyelinInhibitorinhibitorinhibit

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