N6-Methyl-dATP
N6-Methyl-dATP is a dATP (HY-136648) derivative. N6-Methyl-dATP is hydrolyzed by MTH1 to its monophosphate form and eliminated from the nucleotide pool, thereby preventing methylated purines from being incorporated into DNA. N6-Methyl-dATP can be incorporated into DNA by eukaryotic DNA polymerases.
For research use only. We do not sell to patients.
- CAS No.: 5085-65-4
- Formula: C11H18N5O12P3
- Molecular Weight:505.21
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
N6-Methyl-dATP (0-300 µM) is hydrolyzed by purified MTH1 with a kcat of 2.0 s−1, a Km of 40.9 µM, and a kcat/Km of 50,300 M−1 s−1[1].
N6-Methyl-dATP is a good MTH1 substrate, and N6-methyl-dAMP binds to a hydrophobic active-site subpocket of MTH1 that accommodates the N6-methyl group[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:MTH1 wild-type and MTH1 knockout (nudt1uu1732)[1]
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Dosage:0.3 mM (~2 nL)
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Administration:microinjection into fertilized eggs; single administration
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Result:Microinjected MTH1KO embryos had an approximately two-fold higher relative N6-methyl-dA DNA level than microinjected MTH1WT embryos, normalized to untreated embryos of the respective genotype.
Absolute N6-methyl-dA levels were approximately two-fold higher in injected MTH1KO embryos than untreated MTH1KO embryos, while no difference was observed between untreated and injected MTH1WT embryos.
Both MTH1WT and MTH1KO embryos tolerated injection with the same level of survival as noninjected embryos.
Chemical Information
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CAS No. 5085-65-4
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Molecular Weight 505.21
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Formula C11H18N5O12P3
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SMILES
O=P(O)(O)OP(OP(OC[C@H]1O[C@@H](N2C3=NC=NC(NC)=C3N=C2)C[C@@H]1O)(O)=O)(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Scaletti ER, et al. MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool. The Journal of biological chemistry. 2020 Apr 10;295(15):4761-4772. [Content Brief]
[3]. Kang N, et al. Targeting MutT Homolog 1 (MTH1) for Breast Cancer Suppression by Using a Novel MTH1 Inhibitor MA-24 with Tumor-Selective Toxicity. Pharmaceuticals (Basel, Switzerland). 2024 Feb 23;17(3):291. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
N6-Methyl-dATP Related Classifications
HY-178530 Related Classifications
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