2. Anti-infection Apoptosis NF-κB Metabolic Enzyme/Protease
  3. Flavivirus TNF Receptor NF-κB Apoptosis Ser/Thr Protease
  4. Nafamostat hydrochloride

Nafamostat hydrochloride, an anticoagulant, is a synthetic serine protease inhibitor. Nafamostat hydrochloride has anticancer and antivirus effect. Nafamostat hydrochloride induces apoptosis by up-regulating the expression of tumor necrosis factor receptor-1 (TNFR1). Nafamostat hydrochloride can be used in the development of the pathological thickening of the arterial wall.

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CAS No. : 80251-32-7

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Nafamostat hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Nafamostat hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Aug 1:e02156.  [Abstract]

    Targeted inhibition of granzyme B with Z‐AAD‐CMK and granzyme A with Nafamostat mesylate (NFM) attenuates cell death.

    Nafamostat hydrochloride purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2023 Jun:214:105606.  [Abstract]

    Huh7.5 cells was infected with HCoV-229E (MOI = 3.5) and treated with omicsynin B4 (10 μM), E−64d (1 μM) or nafamostat mesylate (40 μM) at the indicated times. Immunofluorescence staining of the dsRNA (green) were merged with the nuclear Hoechst 33342 dye (blue) using a fluorescence microscopy (200 × ). Mock, the cell control. Veh, the vehicle control. Cpd, compound. NH4Cl, ammonium chloride. B4, omicsynin B4. NM, nafamostat mesylate. hpi, hour post infection.

    Nafamostat hydrochloride purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2023 Jan 5:938:175394.  [Abstract]

    Concentration-inhibition curves for Nafamostat mesylate, sepimostat and diminazene aceturate.

    Nafamostat hydrochloride purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2023 Jan 5:938:175394.  [Abstract]

    Representative examples of current inhibition by 1 μM Nafamostat mesylate.

    Nafamostat hydrochloride purchased from MedChemExpress. Usage Cited in: Viruses. 2022 Sep 12;14(9):2017.

    Efficacy of small molecule inhibitors Remdesivir, Nafamostat mesylate, Nirmatrelvir, and Molnupiravir against B.1BAC-V (green), B.1.617.2BAC-V (orange), and corresponding clinical isolates (CI, grey).

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    Description

    Nafamostat hydrochloride, an anticoagulant, is a synthetic serine protease inhibitor. Nafamostat hydrochloride has anticancer and antivirus effect. Nafamostat hydrochloride induces apoptosis by up-regulating the expression of tumor necrosis factor receptor-1 (TNFR1). Nafamostat hydrochloride can be used in the development of the pathological thickening of the arterial wall[1][2][3][4].

    IC50 & Target

    I-kappaBalpha

     

    In Vitro

    Nafamostat hydrochloride (10-80 μg/mL, 3-48 h) inhibits NF-κB activity by blocking IκBα phosphorylation in MDAPanc-28 cells[1].
    Nafamostat hydrochloride (80 μg/mL, 24-48 h) induces apoptosis by up-regulating the expression of tumor necrosis factor receptor-1 (TNFR1) in MDAPanc-28 cells[1].
    Nafamostat hydrochloride (0.1-10 μM, 24 h) has suppressive effect on invasiveness in Panc-1 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Nafamostat hydrochloride Related Antibodies

    Cell Viability Assay[1]

    Cell Line: MDAPanc-28 cells
    Concentration: 80 μg/mL
    Incubation Time: 24 h, 48 h
    Result: Substantially reduced the cell viability of MDAPanc-28 cells at both 24 hours and 48 hours.

    Cell Invasion Assay[2]

    Cell Line: Panc-1 cells
    Concentration: 0.1 μM, 1 μM, 10 μM
    Incubation Time: 24 h
    Result: Observed significant inhibition in Panc-1-Try clones at concentrations as low as 0.1 mM.

    Western Blot Analysis[1]

    Cell Line: MDAPanc-28 cells
    Concentration: 10 μg/mL, 20 μg/mL ,40 μg/mL, 80 μg/mL
    Incubation Time: 3 h, 8 h, 24 h, 48 h
    Result: Inhibited NF-κB DNA-binding activity and the degradation of IκBα in a dose-dependent manner as well as in a time-dependent manner.
    Inhibited phosphorylation of IκBα in a time-dependent manner.
    In Vivo

    Nafamostat hydrochloride (10 mg/kg, Intraperitoneal injection, once a day for 18 days) exhibits favourable antiviral effects against Zika virus (ZIKV) infection in A129 mice[3].
    Nafamostat hydrochloride (0.5-2.0 mg/mL (dissolved in saline), Intraperitoneal injection, once a day for 7 consecutive days) has inhibitory effect on neointimal formation after balloon injury of the rat carotid wall[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: A129 mice[3]
    Dosage: 10 mg/kg
    Administration: Intraperitoneal injection (i.p.)
    Result: Exhibit delayed lethality and improved survival (40%).
    Animal Model: Balloon injury of the rat carotid wall [4]
    Dosage: 0.5 mg/mL, 1 mg/mL, 2 mg/mL (dissolved in saline)
    Administration: Intraperitoneal injection (i.p.)
    Result: Showed smaller ratios of the neointima/medial area.
    Showed positive but reduced immunoreactivity of the cells in the neointimal.
    Molecular Weight

    420.29

    Formula

    C19H19Cl2N5O2
    CAS No.
    SMILES

    O=C(OC1=CC=C2C=C(C(N)=N)C=CC2=C1)C3=CC=C(NC(N)=N)C=C3.Cl.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    Nafamostat hydrochloride Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nafamostat80251-32-7FlavivirusTNF ReceptorNF-κBApoptosisSer/Thr ProteaseTumor Necrosis Factor ReceptorTNFRNuclear factor-κBNuclear factor-kappaBSerine proteasesSerine endopeptidasesThreonine proteasesMDAPanc-28 cellsPanc-1 cellsA129 miceBalloon injury of the rat carotid wallInhibitorinhibitorinhibit

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