Nanaomycin A
Based on 6 publication(s) in Google Scholar
Nanaomycin A is a selective DNMT3B inhibitor with an IC50 of 500 nM. Nanaomycin A inhibits the in vitro growth of human Plasmodium falciparum with an IC80 of 33.1 nM.
For research use only. We do not sell to patients.
- Purity : 98.13%
- CAS No.: 52934-83-5
- Formula: C16H14O6
- Molecular Weight:302.28
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Nanaomycin A
MoreAll DNA Methyltransferase Isoforms
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Biological Activity
Description
IC50 & Target
[1]|
DNMT3B 500 nM (IC50) |
Plasmodium |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Sf9 | IC50 |
0.5 μM
Compound: 23
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Inhibition of His6-tagged human recombinant DNMT3b expressed in insect Sf9 cells assessed as reduction in DNA methyltransferase activity using 5'-biotinylated 45-bp unmethylated or hemimethylated oligonucleotide substrates and [3H]-AdoMet by liquid scinti
Inhibition of His6-tagged human recombinant DNMT3b expressed in insect Sf9 cells assessed as reduction in DNA methyltransferase activity using 5'-biotinylated 45-bp unmethylated or hemimethylated oligonucleotide substrates and [3H]-AdoMet by liquid scinti
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[PMID: 25406944] |
| Vero | CC50 |
0.7 μM
Compound: Nanaomycin A
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Cytotoxicity in African green monkey Vero cells assessed as reduction in cell viability
Cytotoxicity in African green monkey Vero cells assessed as reduction in cell viability
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[PMID: 32435413] |
In Vitro
Nanaomycin A (10-10000 nM; 72 hours) exhibited significant cytotoxicity in all three cell lines[1].
Nanaomycin A (0.5-5 μM; 72 hours) induced RASSF1A protein expression in A549 cells[1].
Nanaomycin A is a selective inhibitor of DNMT3B that induces genome demethylation and does not affect the enzyme activity of DNMT1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HCT116 (colon), A549 (lung), and HL60 (bone marrow) human tumor cell lines
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Concentration:10, 100, 1000, 10000 nM
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Incubation Time:72 hours
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Result:Had a distinct cytotoxic effect in all three cell lines.
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Cell Line:A549 cells
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Concentration:0.5, 5 μM
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Incubation Time:72 hours
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Result:Revealed induction of RASSF1A protein expression.
Chemical Information
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CAS No. 52934-83-5
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Appearance Solid
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Molecular Weight 302.28
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Formula C16H14O6
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Color Yellow to orange
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SMILES
O=C(O)C[C@H]1CC(C(C2=C3C(O)=CC=C2)=O)=C(C3=O)[C@H](C)O1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (6)
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Journal Impact Factor
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Most Recent
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Antioxid Redox Signal
Oxidative Stress-Induced Hypermethylation of KLF5 Promoter Mediated by DNMT3B Impairs Osteogenesis by Diminishing the Interaction with β-Catenin. [Abstract]2021 Jul;35(1):1-20. PMID: 33588625 -
Biochim Biophys Acta Mol Basis Dis
DNMT3B attenuates the development of thoracic aortic aneurysm and dissection by suppressing TFEB-mediated autophagy in vascular smooth muscle cells. [Abstract]2026 Jun 24;1872(7):168331. PMID: 42342163 -
Cancers (Basel)
DNMT3B Controls Enhancer-Linked Chromatin and Cell Cycle Networks in Acute Myeloid Leukemia. [Abstract]2026 Feb 24;18(5):723. PMID: 41827658 -
J Biol Chem
DNMT3b-mediated CpA methylation facilitates REST binding and gene silencing and exacerbates hippocampal demyelination in diabetic mice. [Abstract]2025 Feb;301(2):108137. PMID: 39730060 -
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Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Kuck D, et al.Nanaomycin A selectively inhibits DNMT3B and reactivates silenced tumor suppressor genes in human cancer cells.Mol Cancer Ther. 2010 Nov;9(11):3015-23. [Content Brief]
[2]. Tanaka Y, et al.Heme-dependent radical generation: possible involvement in antimalarial action of non-peroxide microbial metabolites, nanaomycin A and radicicol.J Antibiot (Tokyo). 1999 Oct;52(10):880-8. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Nanaomycin A Related Classifications
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