Neoruscogenin

Cat. No.: HY-N2253 Purity: 99.24%: FAQs
Data Sheet SDS COA Handling Instructions

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Neoruscogenin, a member of the steroidal sapogenin family, is a high-affinity agonist of the nuclear receptor RORα (NR1F1) (EC₅₀ = 0.11 µM).

For research use only. We do not sell to patients.

Neoruscogenin Chemical Structure

CAS No. : 17676-33-4

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 149	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 149	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 75	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 158	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 269	In-stock	Estimated Time of Arrival: December 31
20 mg	USD 457	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Biological Activity
Purity & Documentation
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Description

Neoruscogenin, a member of the steroidal sapogenin family, is a high-affinity agonist of the nuclear receptor RORα (NR1F1) (EC₅₀ = 0.11 µM)^[1].

In Vitro

Neoruscogenin (0.1-10 μM) increases the cell viability, but has no effect on cell viability at 50 μM, and decreases the cell viability at 100 μM^[2].
Neoruscogenin (0.1 μM, 5 days) promotes C2C12 myoblast differentiation^[2].
Neoruscogenin (0.1 μM, 5 days) activates Akt/mTOR/rpS6 signaling pathway in C2C12 myoblasts, and inhibits the Atrogin-1 and MuRF-1 mRNA levels^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	C2C12 myoblasts
Concentration:	0.1 μM
Incubation Time:	5 days
Result:	Increased protein expression of MyHC and MyOG at 0.1 μM, but did not work as well as 0.1 μM at 5 and 10 μM

In Vivo

Neoruscogenin (3 mg/kg,p.o. for seven days) activates hepatic RORα target gene (such as Bmal1, Cyp7b1, and G6Pase) expression in mice^[1].
Neoruscogenin (1 mg/kg,i.p. every 2 days for 8 times) induces skeletal muscle hypertrophy in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	male ICR mice^[2]
Dosage:	1 mg/kg
Administration:	i.p.
Result:	Showd the larger size of TA muscles, and increasaed the weight of Gas muscles. Showed visible myofibers hypertrophy (H&E staining).

Molecular Weight

428.60

Formula

C₂₇H₄₀O₄

CAS No.

17676-33-4

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]3(OC4)CCC4=C)([H])C[C@@]1([H])[C@@](CC=C(C[C@@H](O)C5)[C@@]6([C@@H]5O)C)([H])[C@]6([H])CC2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (116.66 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 10 mg/mL (23.33 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3332 mL	11.6659 mL	23.3318 mL
5 mM	0.4666 mL	2.3332 mL	4.6664 mL
10 mM	0.2333 mL	1.1666 mL	2.3332 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.83 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.56 mg/mL (1.31 mM); Clear solution

This protocol yields a clear solution of ≥ 0.56 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.6 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.56 mg/mL (1.31 mM); Clear solution

This protocol yields a clear solution of ≥ 0.56 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.6 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.24%

Select Batch:

Data Sheet (288 KB) SDS (251 KB)

COA (261 KB) HNMR (277 KB) RP-HPLC (200 KB) MS (90 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	2.3332 mL	11.6659 mL	23.3318 mL	58.3294 mL
	5 mM	0.4666 mL	2.3332 mL	4.6664 mL	11.6659 mL
	10 mM	0.2333 mL	1.1666 mL	2.3332 mL	5.8329 mL
	15 mM	0.1555 mL	0.7777 mL	1.5555 mL	3.8886 mL
	20 mM	0.1167 mL	0.5833 mL	1.1666 mL	2.9165 mL
DMSO	25 mM	0.0933 mL	0.4666 mL	0.9333 mL	2.3332 mL
	30 mM	0.0778 mL	0.3889 mL	0.7777 mL	1.9443 mL
	40 mM	0.0583 mL	0.2916 mL	0.5833 mL	1.4582 mL
	50 mM	0.0467 mL	0.2333 mL	0.4666 mL	1.1666 mL
	60 mM	0.0389 mL	0.1944 mL	0.3889 mL	0.9722 mL
	80 mM	0.0292 mL	0.1458 mL	0.2916 mL	0.7291 mL
	100 mM	0.0233 mL	0.1167 mL	0.2333 mL	0.5833 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Neoruscogenin17676-33-4Inhibitorinhibitorinhibit

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