NF-κΒ activator 1
Based on 17 publication(s) in Google Scholar
NF-κΒ activator 1 (compound 32) is a potent NF-κΒ activator with an EC50 of 0.9 μM. NF-κΒ activator 1 induces superoxide dismutase (SOD)2 mRNA expression.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.59%
- No. CAS: 2387524-59-4
- Fòrmula: C16H11FN2O2S
- Peso molecular:314.33
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) NF-κΒ activator 1
More- Imeta. 2026 Jun 14.
- J Clin Invest. 2024 Oct 1;134(22):e178628. [Abstract]
- Carbohydr Polym. 2024 Oct 15:342:122422. [Abstract]
- Chem Eng J. 2024 May 1, 487, 150501.
- Cell Death Dis. 2023 Nov 15;14(11):743. [Abstract]
- Oncogene. 2024 Sep;43(38):2835-2849. [Abstract]
- J Nutr Biochem. 2026 May 25:110423. [Abstract]
- J Mol Cell Cardiol. 2025 May:202:133-143. [Abstract]
- J Immunol. 2025 Jun 28:vkaf125. [Abstract]
- Clin Exp Immunol. 2025 Jan 21;219(1):uxaf055. [Abstract]
- Int J Med Sci. 2026 Feb 18;23(3):1092-1104. [Abstract]
- Toxicol Appl Pharmacol. 2026 Aug:513:117836. [Abstract]
- Mol Oral Microbiol. 2026 Aug;41(4):216-226. [Abstract]
- J Cancer. 2025 Jan 1;16(3):1008-1019. [Abstract]
- Behav Brain Res. 2024 Mar 12:461:114837. [Abstract]
- Thromb J. 2025 May 20;23(1):51. [Abstract]
- Anim Reprod Sci. 2026 Jun:289:108144. [Abstract]
Customer Validation & Images
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WB
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Cell Imaging/Staining
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Cell Proliferation/Viability Assay
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WB
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RT-PCR
Actividad biológica
Descripciòn
IC50 & Target
[1]|
NF-κB 0.9 μM (EC50) |
In Vitro
NF-κΒ activator 1 (compound 32) (1 μM; 6 hours) induces SOD2 mRNA expression in SH-SY5Y neuroblastoma cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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No. CAS 2387524-59-4
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Appearance Solid
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Peso molecular 314.33
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Fòrmula C16H11FN2O2S
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Color Light yellow to yellow
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SMILES
O=C(O)C1=CC=CC(C2=CSC(NC3=CC=CC=C3F)=N2)=C1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (17)
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Journal Impact Factor
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Most Recent
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J Clin Invest
Dual targeting macrophages and microglia is a therapeutic vulnerability in models of PTEN-deficient glioblastoma. [Abstract]2024 Oct 1;134(22):e178628. PMID: 39352749 -
Carbohydr Polym
A novel strategy with in vivo characterization, extraction, isolation and activity evaluation for discovery of absorbed anti-inflammatory oligosaccharides from Zhu-Ling decoction. [Abstract]2024 Oct 15:342:122422. PMID: 39048245 -
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Cell Death Dis
BRISC is required for optimal activation of NF-κB in Kupffer cells induced by LPS and contributes to acute liver injury. [Abstract]2023 Nov 15;14(11):743. PMID: 37968261
NF-κΒ activator 1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Nov 15;14(11):743. [Abstract]
WT and Abro1 KCs cells were pretreated with NF-κB activator 1 for 6 hours, followed by treatment with 100 ng/ml LPS for 3 hours. CBA analysis was used to analyze the expression of TNF-α and IL-6.
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Oncogene
Monoacylglycerol lipase blockades the senescence-associated secretory phenotype by interfering with NF-κB activation and promotes docetaxel efficacy in prostate cancer. [Abstract]2024 Sep;43(38):2835-2849. PMID: 39155296
NF-κΒ activator 1 purchased from MedChemExpress. Usage Cited in: Oncogene. 2024 Sep;43(38):2835-2849. [Abstract]
NF-κΒ activator 1 (1 μM for 48 h). Immunoblot detection of NF-κB p105/50, NF-κB p100, IκBα, Phospho-IκBα (Ser32/36), NF-κB p65 and phospho-NF-κB p65 (Ser536) in Over-MGLL and Vector PC3 cells.
NF-κΒ activator 1 purchased from MedChemExpress. Usage Cited in: Oncogene. 2024 Sep;43(38):2835-2849. [Abstract]
NF-κB activator 1 (1 μM for 48 h). Staining for aging-related β-galactosidase.
NF-κΒ activator 1 purchased from MedChemExpress. Usage Cited in: Oncogene. 2024 Sep;43(38):2835-2849. [Abstract]
Cell proliferation assays of MGLL-overexpressing stable cells, docetaxel (10 nM), and NF-κB activator 1 (1 μM, 48 h) treated with these agents.
NF-κΒ activator 1 purchased from MedChemExpress. Usage Cited in: Oncogene. 2024 Sep;43(38):2835-2849. [Abstract]
Immunoblotting results of MGLL overexpression stable cells treated with docetaxel (10 nM) and NF-κB activator 1 (1 μM, 48 h).
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J Nutr Biochem
Quercetin reverses DEHP-induced ZBP1-mediated PANoptosis in thymus by targeting NF-κB to regulate the TNF-α/ROS signaling pathway. [Abstract]2026 May 25:110423. PMID: 42191001 -
J Mol Cell Cardiol
Elevated KCa3.1 expression by angiotensin II via the ERK/NF-κB pathway contributes to atrial fibrosis. [Abstract]2025 May:202:133-143. PMID: 40122157 -
J Immunol
2025 Jun 28:vkaf125. PMID: 40580518 -
Clin Exp Immunol
Docosahexaenoic acid mitigates experimental autoimmune prostatitis by inhibiting Th17 cell differentiation via the PPARγ/NF-κB/IL-17A pathway. [Abstract]2025 Jan 21;219(1):uxaf055. PMID: 40887935 -
Int J Med Sci
Artemisia argyi-enhanced Mesenchymal Stem Cell Exosomes Alleviates Inflammation in C28/I2 Chondrocytes by inhibiting NF-κB. [Abstract]2026 Feb 18;23(3):1092-1104. PMID: 41799761 -
Toxicol Appl Pharmacol
ADAMTS7 selective inhibitor BAY-9835 alleviates acute myocardial infarction by suppressing the NF-κB-mediated pyroptosis. [Abstract]2026 Aug:513:117836. PMID: 42061659 -
Mol Oral Microbiol
Ginsenoside Compound K Plays an Anti-Inflammatory Role in Gingival Epithelial Cells Stimulated With Porphyromonas gingivalis by Regulating Pyruvate Kinase M2. [Abstract]2026 Aug;41(4):216-226. PMID: 41923291 -
J Cancer
ADAMTS7 Enhances Gastric Cancer Growth and Metastasis by Triggering the NF-κB Signaling Pathway. [Abstract]2025 Jan 1;16(3):1008-1019. PMID: 39781347 -
Behav Brain Res
2024 Mar 12:461:114837. PMID: 38145872 -
Thromb J
Liquiritin mitigates lower extremity deep vein thrombosis by inhibiting inflammation and oxidative stress via the NF-κB signaling pathway. [Abstract]2025 May 20;23(1):51. PMID: 40394684 -
Anim Reprod Sci
HSP72 alleviates heat stress-induced oxidative damage in boar Sertoli cells through modulating the AGE-RAGE/NOX4/NF-κB axis. [Abstract]2026 Jun:289:108144. PMID: 41740418
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (318.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (6.62 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (6.62 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
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RT-PCR
Reverse transcription technology uses RNA as a template to synthesize DNA. RT-PCR is simple, specific and sensitive, and can be used to detect gene expression levels and expression differences in cells; detect RNA virus content; clone cDNA sequences of specific genes.
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Real Time qPCR (Q-PCR)
Real-time quantitative PCR (qPCR) quantifies an amplifiable nucleic-acid target by monitoring fluorescence during PCR cycling rather than measuring product only after amplification. The increase in fluorescence tracks accumulation of PCR product, and the quantification cycle (Cq; historically also Ct/CP) is related to the initial amount of target: samples containing more starting target generally reach the defined fluorescence threshold in fewer cycles.
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.1814 mL | 15.9069 mL | 31.8137 mL | 79.5343 mL |
| 5 mM | 0.6363 mL | 3.1814 mL | 6.3627 mL | 15.9069 mL | |
| 10 mM | 0.3181 mL | 1.5907 mL | 3.1814 mL | 7.9534 mL | |
| 15 mM | 0.2121 mL | 1.0605 mL | 2.1209 mL | 5.3023 mL | |
| 20 mM | 0.1591 mL | 0.7953 mL | 1.5907 mL | 3.9767 mL | |
| 25 mM | 0.1273 mL | 0.6363 mL | 1.2725 mL | 3.1814 mL | |
| 30 mM | 0.1060 mL | 0.5302 mL | 1.0605 mL | 2.6511 mL | |
| 40 mM | 0.0795 mL | 0.3977 mL | 0.7953 mL | 1.9884 mL | |
| 50 mM | 0.0636 mL | 0.3181 mL | 0.6363 mL | 1.5907 mL | |
| 60 mM | 0.0530 mL | 0.2651 mL | 0.5302 mL | 1.3256 mL | |
| 80 mM | 0.0398 mL | 0.1988 mL | 0.3977 mL | 0.9942 mL | |
| 100 mM | 0.0318 mL | 0.1591 mL | 0.3181 mL | 0.7953 mL |
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