2. Stem Cell/Wnt
  3. Large Tumor Suppressor (LATS) YAP
  4. NIBR-LTSi

NIBR-LTSi is an orally active and selective LATS kinase inhibitor. NIBR-LTSi can activate YAP signaling. NIBR-LTSi promotes stem cell proliferation, maintains stemness and blocks differentiation. NIBR-LTSi accelerates liver regeneration following extended hepatectomy in mice.

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Customer Review

Based on 1 publication(s) in Google Scholar

NIBR-LTSi Related Antibodies

Top Publications Citing Use of Products
WB
Cell Imaging/Staining

    NIBR-LTSi purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Cell Res. 2025 Feb 17;1872(3):119921.  [Abstract]

    NIBR-LTSi (2.5 μM). Western blot results of YAP and the senescence-related genes P21 and apoptosis-related genes BCL2 and BAX in PDLSCs.

    NIBR-LTSi purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Cell Res. 2025 Feb 17;1872(3):119921.  [Abstract]

    NIBR-LTSi (2.5 μM). Microscopic observation and quantitative analysis of β-galactosidase-positive stained PDLSCs were performed. NC: P20 PDLSCs. +S: P20 PDLSCs were treated with senolytics. +S+N: P20 PDLSCs treated with senolytics plus NIBR-LTSi.

    View All YAP Isoform Specific Products:

    View All Isoforms
    YAP/TAZ YAP/TAZ-TEAD TEAD YAP1

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    NIBR-LTSi is an orally active and selective LATS kinase inhibitor. NIBR-LTSi can activate YAP signaling. NIBR-LTSi promotes stem cell proliferation, maintains stemness and blocks differentiation. NIBR-LTSi accelerates liver regeneration following extended hepatectomy in mice[1].

    IC50 & Target

    LATS[1]
    In Vitro

    NIBR-LTSi (0.1-10 μM; 2 h) inhibits YAP phosphorylation in JHH5 cells with an IC50 of 2.16 μM[1].
    NIBR-LTSi (1-10 μM; 3-10 days) promotes keratinocyte proliferation, increases epidermal thickness and blocks keratinocyte differentiation in human 3D skin model[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    NIBR-LTSi Related Antibodies
    In Vivo

    NIBR-LTSi (30-100 mg/kg; oral administration; single dose) promotes hepatocyte proliferation and liver regeneration in a partial hepatectomy mouse model[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Partial hepatectomy treated C57BL/6 WT mice[1]
    Dosage: 30 and 100 mg/kg
    Administration: Oral administration (p.o.); single dose
    Result: Significantly induced hepatocyte proliferation in a dose-dependent manner.
    Molecular Weight

    308.38

    Formula

    C18H20N4O
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C1(NC2=CC=C(C=C2)O[C@H]3CNCCC3)=CC=NC4=C1C=CN4
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (324.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.2428 mL 16.2138 mL 32.4275 mL
    5 mM 0.6486 mL 3.2428 mL 6.4855 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (16.21 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 5 mg/mL (16.21 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.97%

    SDS (251 KB)

    COA (248 KB) HNMR (151 KB) RP-HPLC (169 KB) LCMS (236 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.2428 mL 16.2138 mL 32.4275 mL 81.0688 mL
    5 mM 0.6486 mL 3.2428 mL 6.4855 mL 16.2138 mL
    10 mM 0.3243 mL 1.6214 mL 3.2428 mL 8.1069 mL
    15 mM 0.2162 mL 1.0809 mL 2.1618 mL 5.4046 mL
    20 mM 0.1621 mL 0.8107 mL 1.6214 mL 4.0534 mL
    25 mM 0.1297 mL 0.6486 mL 1.2971 mL 3.2428 mL
    30 mM 0.1081 mL 0.5405 mL 1.0809 mL 2.7023 mL
    40 mM 0.0811 mL 0.4053 mL 0.8107 mL 2.0267 mL
    50 mM 0.0649 mL 0.3243 mL 0.6486 mL 1.6214 mL
    60 mM 0.0540 mL 0.2702 mL 0.5405 mL 1.3511 mL
    80 mM 0.0405 mL 0.2027 mL 0.4053 mL 1.0134 mL
    100 mM 0.0324 mL 0.1621 mL 0.3243 mL 0.8107 mL
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    NIBR-LTSi Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    NIBR-LTSiLarge Tumor Suppressor (LATS)YAPLarge Tumor SuppressorYes-associated proteinstem cellYAP/Hippo pathwayJHH5 cellsInhibitorinhibitorinhibit

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