Cat. No.: HY-111793A

NUCC-390 dihydrochloride

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

NUCC-390 dihydrochloride is a novel and selective small-molecule CXCR4 receptor agonist. NUCC-390 dihydrochloride induces internalization of CXCR4 receptors and acts in an opposite way of AMD3100 (HY-10046). NUCC-390 dihydrochloride promotes nerve recovery of function after neurodegeneration in vivo.

For research use only. We do not sell to patients.

Purity: 98.19%
CAS No.: 2749281-71-6
Formula: C₂₃H₃₅Cl₂N₅O
Molecular Weight:468.46

Storage:

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

NUCC-390 dihydrochloride Chemical Structure

CAS No.: 2749281-71-6

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

NUCC-390 dihydrochloride is a novel and selective small-molecule CXCR4 receptor agonist. NUCC-390 dihydrochloride induces internalization of CXCR4 receptors and acts in an opposite way of AMD3100 (HY-10046)^[1]. NUCC-390 dihydrochloride promotes nerve recovery of function after neurodegeneration in vivo^[2].

IC₅₀ & Target^[1]

CXCR4

All CXCR Isoforms :

In Vitro

NUCC-390 dihydrochloride (10 μM) produces strong (Ca)i response, but this effect can be blocked by the known potent and selective CXCR4 antagonist AMD3100^[1].
NUCC-390 dihydrochloride (10 μM; pre-treatment 30 mins) leads to increased levels of pERK, it has the capability of stimulating signaling activity downstream of CXCR4 receptors^[1].
NUCC-390 dihydrochloride (10 μM; 2 hours) can induce CXCR4 receptor internalization, and non-treated cells exhibit some diffuse expression of CXCR4-YFP throughout the cytosol and clear expression in the cell membrane in HEK cells^[1].
NUCC-390 dihydrochloride (0-1.25 μM; 24 hours) boosts axonal growth in cultured cerebellar granule neurons (CGNs) via CXCR4^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

C8161 cells
Concentration:

10 μM
Incubation Time:

Pre-treated 30 mins
Result:

Increased the level of pERK.

Cell Proliferation Assay^[2]

Cell Line:

Cerebellar granule neurons (CGNs)
Concentration:

0 μM; 0.0625 μM; 0.25 μM; 1.25 μM
Incubation Time:

24 hours
Result:

Stimulated axonal growth via CXCR4.

In Vivo

NUCC-390 dihydrochloride (hind limb injection; 3.2 mg/kg; twice daily; 3 days) contributes to the functional and anatomical recovery of the neuromuscular junction (NMJ) following an acute nerve terminal damage by α-LTx in CD-1 mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Six to eight-week-old CD1 mice^[2]
Dosage:

3.2 mg/kg
Administration:

Hind limb injection; twice daily; 3 days
Result:

Promoted functional and anatomical recovery of the NMJ.

Chemical Information

CAS No. 2749281-71-6
Appearance Solid
Molecular Weight 468.46
Formula C₂₃H₃₅Cl₂N₅O
Color Light yellow to yellow
SMILES

O=C(N1CCCCC1)C2=NN(CCC)C3=C2CC(NCCC4=CC=NC=C4)CC3.Cl.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Publications (6)

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Journal Impact Factor

Proc Natl Acad Sci U S A

CXCL12 restricts tumor growth by suppressing the Ras, ERK1/2, c-Myc, and the immune checkpoint PD-L1 pathways. [Abstract]

2024 Dec 24;121(52):e2416909121. PMID: 39689179
Int Immunopharmacol

Astragalin alleviates neuroinflammation in LPS-injured HT22 cells and CFA mice by regulating neuronal excitability and autophagy via CXCR4-Beclin1/VPS34 axis. [Abstract]

2026 Mar 1:172:116227. PMID: 41548438
Int Immunopharmacol

Isoferulic acid regulates CXCL12/CXCR4-mediated apoptosis and autophagy in podocyte and mice with STZ-induced diabetic nephropathy. [Abstract]

2025 Jan 10:144:113707. PMID: 39616856
Microsc Microanal

The Role of the CXCL12/CXCR4 Signaling Pathway in Regulating Cellular Migration. [Abstract]

2025 Mar 17;31(2):ozaf011. PMID: 40095909
Neurosci Res

CXCR4 signaling regulates repair Schwann cell infiltration into the spinal cord after spinal cord injury in mice. [Abstract]

2023 Jun:191:38-47. PMID: 36592826
Arab J Gastroenterol

C-X-C motif chemokine receptor 4 inhibition promotes the effect of plantamajoside in hepatocellular carcinoma. [Abstract]

2024 Feb;25(1):28-36. PMID: 38220479

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (213.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (53.37 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1347 mL

10.6733 mL

21.3465 mL

5 mM

0.4269 mL

2.1347 mL

4.2693 mL

10 mM

0.2135 mL

1.0673 mL

2.1347 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 20 mg/mL (42.69 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Mishra RK, et al. Discovery and characterization of novel small-molecule CXCR4 receptor agonists and antagonists.Sci Rep. 2016 Jul 26;6:30155. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.1347 mL	10.6733 mL	21.3465 mL	53.3664 mL
	5 mM	0.4269 mL	2.1347 mL	4.2693 mL	10.6733 mL
	10 mM	0.2135 mL	1.0673 mL	2.1347 mL	5.3366 mL
	15 mM	0.1423 mL	0.7116 mL	1.4231 mL	3.5578 mL
	20 mM	0.1067 mL	0.5337 mL	1.0673 mL	2.6683 mL
	25 mM	0.0854 mL	0.4269 mL	0.8539 mL	2.1347 mL
	30 mM	0.0712 mL	0.3558 mL	0.7116 mL	1.7789 mL
	40 mM	0.0534 mL	0.2668 mL	0.5337 mL	1.3342 mL
	50 mM	0.0427 mL	0.2135 mL	0.4269 mL	1.0673 mL
DMSO	60 mM	0.0356 mL	0.1779 mL	0.3558 mL	0.8894 mL
	80 mM	0.0267 mL	0.1334 mL	0.2668 mL	0.6671 mL
	100 mM	0.0213 mL	0.1067 mL	0.2135 mL	0.5337 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

NUCC-390 dihydrochloride

6 Cited Publications

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NUCC-390 dihydrochloride

Bioactive Compound Library Max

Bioactive Compound Library

GPCR/G Protein Compound Library

Immunology/Inflammation Compound Library

Small Molecule Immuno-Oncology Compound Library

Targeted Diversity Library

Chemokine Compound Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Activators Library

Extracellular Vesicles (EVs) Compound Library

MCE Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) NUCC-390 dihydrochloride

All CXCR Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

NUCC-390 dihydrochloride

Bioactive Compound Library Max

Bioactive Compound Library

GPCR/G Protein Compound Library

Immunology/Inflammation Compound Library

Small Molecule Immuno-Oncology Compound Library

Targeted Diversity Library

Chemokine Compound Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Activators Library

Extracellular Vesicles (EVs) Compound Library

MCE Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) NUCC-390 dihydrochloride

All CXCR Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

NUCC-390 dihydrochloride Related Classifications

HY-111793A Related Classifications