2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. ODN 1018

ODN 1018 (1018 ISS) is a TLR9 agonist and immune modulator. ODN 1018 exhibits adjuvant activity and augments CD8+ T cell responses with LNP-encapsulated OVA peptides. ODN 1018 triggers sustained suppression of allergic airway inflammation. ODN 1018 can be used for the research of allergic asthma and systemic lupus erythematosus.

For research use only. We do not sell to patients.

CAS No. : 937402-51-2

Size Price Stock Quantity
1 mg In-stock
5 mg In-stock
10 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of ODN 1018:

ODN 1018 Related Antibodies

Top Publications Citing Use of Products
In Vivo Efficacy Study

    ODN 1018 purchased from MedChemExpress. Usage Cited in: Nature. 2025 May;641(8061):202-210.  [Abstract]

    Mice were immunized with ABM5-OVA, or OVA peptide with or without 10 μg of ODN1018, ISCOMs or poly-I:C on days 0 and 14.

    ODN 1018 purchased from MedChemExpress. Usage Cited in: Nature. 2025 May;641(8061):202-210.  [Abstract]

    Mice were immunized with ABM5-OVA, or OVA peptide with or without 10 μg of ODN1018, ISCOMs or poly-I:C on days 0 and 28.

    View All Toll-like Receptor (TLR) Isoform Specific Products:

    View All Isoforms
    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    ODN 1018 (1018 ISS) is a TLR9 agonist and immune modulator. ODN 1018 exhibits adjuvant activity and augments CD8+ T cell responses with LNP-encapsulated OVA peptides. ODN 1018 triggers sustained suppression of allergic airway inflammation. ODN 1018 can be used for the research of allergic asthma and systemic lupus erythematosus[1][2][3].

    IC50 & Target

    TLR9

     

    In Vitro

    ODN 1018 (0.7 μM) stimulates proliferation and IL-6 production in purified human B cells, and this stimulation is inhibited by IRS at 0.35-0.7 μM[3].
    ODN 1018 (0.7 μM; 48 h) activates mouse splenocytes to produce IL-6 via TLR9, and this activation is dose-dependently inhibited by IRS 869 at 0.0875-0.7 μM[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ODN 1018 Related Antibodies
    In Vivo

    ODN1018 (10 μg per mouse; s.c.; days 0 and 14) acts as an adjuvant to enhance OVA-specific CD8+ T cell responses by 7-fold in C57BL/6 mice[1].
    ODN 1018 (20 μg; intranasally; weekly; 1-17 weeks) induces sustained suppression of allergic airway inflammation in ragweed-sensitized mice, with 5 weekly doses sufficient for durable effects associated with TReg induction[2].
    ODN 1018 (50 μg; s.c.) induces a lethal TLR9-mediated inflammatory response in D-galactosamine-pretreated BALB/c mice[3].
    ODN 1018 (25 μg; s.c.) stimulates TLR9-mediated cytokine (IL-6, IL-12) production in BALB/c mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/c (6-8 week old female, ragweed-sensitized allergic asthma model)[2]
    Dosage: 20 μg
    Administration: intranasally; weekly; 1, 3, 5, 8, 12, 13, 16, or 17 weeks
    Result: Suppressed bronchoalveolar lavage (BAL) eosinophilia, BAL IL-4, IL-5, IL-13 levels, and lung Th2-associated genes (Il13, Ccl11, Gob5, Fizz1) through 13 subsequent weekly ragweed exposures (12 weekly treatments).
    Induced significant, lasting reduction of Th2-mediated responses (5 weekly treatments); Upregulated lung regulatory T cell (TReg)-associated genes (Foxp3, Il10, Tgfb1, Lag3, Tnfrsf4, Il2ra) and increased TReg activity (CD4+CD25+ T cells from treated mice suppressed CD4+CD25- T cell proliferation more effectively than those from ragweed-only mice) (16 weekly treatments).
    Induced a moderate Th1 shift (progressive increase in lung Ifng, Cxcl9, Cxcl10 expression) but Th2 suppression did not require IFN-γ; Prevented ragweed-pulsed bone marrow dendritic cells from restoring Th2 responses.
    Molecular Weight

    7150.00

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    DNA, d(P-thio)(T-G-A-C-T-G-T-G-A-A-C-G-T-T-C-G-A-G-A-T-G-A)
    SMILES

    [DNA, d(P-thio)(T-G-A-C-T-G-T-G-A-A-C-G-T-T-C-G-A-G-A-T-G-A)]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 100 mg/mL (13.99 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.1399 mL 0.6993 mL 1.3986 mL
    5 mM 0.0280 mL 0.1399 mL 0.2797 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.28%

    SDS (252 KB)

    COA (239 KB) LCMS (318 KB)

    Handling Instructions (2242 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 0.1399 mL 0.6993 mL 1.3986 mL 3.4965 mL
    5 mM 0.0280 mL 0.1399 mL 0.2797 mL 0.6993 mL
    10 mM 0.0140 mL 0.0699 mL 0.1399 mL 0.3497 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    ODN 1018 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ODN 1018937402-51-21018 ISSODN1018ODN-1018Toll-like Receptor (TLR)TLR9Th2-mediated immune responseshuman B cellsOVA peptidesCD8+ T cellTh1 immune responsesragweed allergenmouse splenocytesregulatory T cellmiceInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    ODN 1018
    Cat. No.:
    HY-150724
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Korean - KR (252 KB) Portuguese - PT (252 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.