Cat. No.: HY-N0515

Ophiopogonin D

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Ophiopogonin D can be isolated from the tubers of Ophiopogon japonicus, is a rare naturally occurring C₂₉ steroidal glycoside. Ophiopogonin D is a CYP2J3 inducer that significantly inhibits Ang II induced NF-κB nuclear translocation, IκBα down-regulation, intracellular Ca²⁺ overload and activation of pro-inflammatory cytokines by increasing the expression of CYP2J2/EETs and PPARα in human umbilical vein endothelial cells (HUVECs). Ophiopogonin D can inhibit isteoclastic differentiation in RAW264.7 cells. Ophiopogonin D has protective effect as an antioxidant in H₂O₂-induced endothelial injury. Ophiopogonin D blocks ERK signaling cascades. Ophiopogonin D alleviates high-fat diet-induced metabolic syndrome and changes the structure of gut microbiota in mice. Ophiopogonin D has been used against inflammatory, metabolic and cardiovascular diseases.

For research use only. We do not sell to patients.

Purity: 99.80%
CAS No.: 945619-74-9
Formula: C₄₄H₇₀O₁₆
Molecular Weight:855.02

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Ophiopogonin D Chemical Structure

CAS No.: 945619-74-9

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

PPARα

NF-κB

All PPAR Isoforms :

All NF-κB Isoforms :

All Calcium Channel Isoforms :

All ERK Isoforms :

In Vitro

Ophiopogonin D (Compound OP-D) (0.1-200 μM, 24-48 h) only provokes cell viability at high concentrations above 40 μM in HUVECs^[2].
Ophiopogonin D (5-20 μM, 24 h) significantly increases the expression of CYP2J2 and PPARα protein and mRNA expression dose-dependently^[2].
Ophiopogonin D (1-100 μM, 24 h prior to H₂O₂) inhibits H₂O₂-induced cytotoxicity in MC3T3-E1 and RAW264.7 cells^[3].
Ophiopogonin D (1-100 μM) inhibits osteoclastic differentiation in RAW264.7 cells^[3].
Ophiopogonin D (0.6-60 μM, 2 h) dose-dependently prevents H₂O₂-induced oxidative stress in HUVECs^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[2]

Cell Line:

HUVECs
Concentration:

5, 10, 20 μM
Incubation Time:

24 h
Result:

Significantly increased 11,12-DHET levels.

Western Blot Analysis^[2]

Cell Line:

HUVECs
Concentration:

5, 10, 20 μM
Incubation Time:

24 h
Result:

Suppressed Ang II-induced inflammatory responses via CYP2J2-PPARα pathway.

In Vivo

Ophiopogonin D (Compound OP-D) (5-25 mg/kg, i.p., daily for 12 w) partially ameliorates the increased SMI and Tb.Sp that attributes to the ovariectomy in OVX mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c female OVX mice (19-21 g)^[3]
Dosage:

5, 25 mg/kg
Administration:

Intraperitoneal injection (i.p.), daily for 12 w
Result:

Inhibited serum osteoclastic markers in serum, such as CTX-1 and TRAP.
Partially inhibited osteoclastogenesis.

Chemical Information

CAS No. 945619-74-9
Appearance Solid
Molecular Weight 855.02
Formula C₄₄H₇₀O₁₆
Color White to off-white
SMILES

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])C[C@@]1([H])[C@@](CC=C5[C@@]6([C@@H](C[C@H](O)C5)O[C@@](O[C@H](C)[C@H](O)[C@@H]7O[C@@](OC[C@@H](O)[C@@H]8O)([H])[C@@H]8O)([H])[C@@H]7O[C@@](O[C@@H](C)[C@H](O)[C@H]9O)([H])[C@@H]9O)C)([H])[C@]6([H])CC2
Structure Classification
- Steroids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (2)

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Journal Impact Factor

Part Fibre Toxicol

Exposure to polylactic acid microplastics during puberty increases the risk of skeletal dysplasia by disrupting arachidonic acid metabolism in osteoblasts. [Abstract]

2026 Apr 22;23(1):26. PMID: 42021360
Int Immunopharmacol

Ophiopogonin D reprograms the polarization of macrophages through modulating PPM1K-mediated branched-chain amino acids catabolism to delay atherogenesis. [Abstract]

2026 May 15:177:116551. PMID: 41871493

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (116.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.1696 mL

5.8478 mL

11.6956 mL

5 mM

0.2339 mL

1.1696 mL

2.3391 mL

10 mM

0.1170 mL

0.5848 mL

1.1696 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wang L , et al. Homo-aro-cholestane, furostane and spirostane saponins from the tubers of Ophiopogonjaponicus. Phytochemistry. 2017 Apr;136:125-132. [Content Brief]

[2]. Huang X, et al. Ophiopogonin D and EETs ameliorate Ang II-induced inflammatory responses via activating PPARα in HUVECs. Biochem Biophys Res Commun. 2017 Aug 19;490(2):123-133. [Content Brief]

[3]. Huang, Q., et al., (2015). Ophiopogonin D: A new herbal agent against osteoporosis. Bone, 74, 18–28. [Content Brief]

[4]. Qian, J., et al., (2010). Ophiopogonin D prevents H2O2-induced injury in primary human umbilical vein endothelial cells. Journal of ethnopharmacology, 128(2), 438–445. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.1696 mL	5.8478 mL	11.6956 mL	29.2391 mL
	5 mM	0.2339 mL	1.1696 mL	2.3391 mL	5.8478 mL
	10 mM	0.1170 mL	0.5848 mL	1.1696 mL	2.9239 mL
	15 mM	0.0780 mL	0.3899 mL	0.7797 mL	1.9493 mL
	20 mM	0.0585 mL	0.2924 mL	0.5848 mL	1.4620 mL
	25 mM	0.0468 mL	0.2339 mL	0.4678 mL	1.1696 mL
	30 mM	0.0390 mL	0.1949 mL	0.3899 mL	0.9746 mL
	40 mM	0.0292 mL	0.1462 mL	0.2924 mL	0.7310 mL
	50 mM	0.0234 mL	0.1170 mL	0.2339 mL	0.5848 mL
	60 mM	0.0195 mL	0.0975 mL	0.1949 mL	0.4873 mL
	80 mM	0.0146 mL	0.0731 mL	0.1462 mL	0.3655 mL
	100 mM	0.0117 mL	0.0585 mL	0.1170 mL	0.2924 mL

Keywords

Ophiopogonin D

2 Cited Publications

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Ophiopogonin D

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Publications Citing Use of MedChemExpress (MCE) Ophiopogonin D

All PPAR Isoforms

All NF-κB Isoforms

All Calcium Channel Isoforms

All ERK Isoforms

Biological Activity

Chemical Information

Publications (2)