Oleuropein

Name: Oleuropein
Brand: MedChemExpress
SKU: HY-N0292
Rating: 5.0 (9 reviews)

Cat. No.: HY-N0292 Purity: 99.97%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Oleuropein, found in olive leaves and oil, exerts antioxidant, anti-inflammatory and anti-atherogenic effects through direct inhibition of PPARγ transcriptional activity. Oleuropein induces apoptosis in breast cancer cells via the p53-dependent pathway and through the regulation of Bax and Bcl2 genes. Oleuropein also inhibits aromatase.

For research use only. We do not sell to patients.

CAS No. : 32619-42-4

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Oleuropein:

Oleuropein (Standard) In-stock

9 Publications Citing Use of MCE Oleuropein

Cell Imaging/Staining

In Vivo Imaging

Bio/Physico-chemical Assay

2D/3D Cell Culture and Differentiation

In Vivo Efficacy Study

: Oleuropein purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Feb 5;15(1):1087. [Abstract]; IHC experiment detecting OCT4 expression in vehicle- or Oleuropein (0.5 μg/mL)-treated WT and aneuploid primary teratomas.

: Oleuropein purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Feb 5;15(1):1087. [Abstract]; BLI imaging of distant organs from teratoma-bearing mice of different aneuploidy exposure to proteasome activator Oleuropein (20 mg/kg/day, i.p.).

: Oleuropein purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Feb 5;15(1):1087. [Abstract]; Oleuropein (0.5 μg/mL) treatment could increase the proteasome activity in aneuploid cells to some extent.

: Oleuropein purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Feb 5;15(1):1087. [Abstract]; In vitro EB differentiation of WT and aneuploid ESCs with or without Oleuropein (0.5 μg/mL) treatment.

: Oleuropein purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Feb 5;15(1):1087. [Abstract]; Treatment with Oleuropein (20 mg/kg/day, i.p.) effectively reduced aneuploid teratoma volume.

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View All Cytochrome P450 Isoform Specific Products:

View All Isoforms

CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A CYP3A4 CYP17A1

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PPARα PPARβ/δ PPARγ PPAR PPARδ

Biological Activity
Purity & Documentation
References
Customer Review

Description

Oleuropein, found in olive leaves and oil, exerts antioxidant, anti-inflammatory and anti-atherogenic effects through direct inhibition of PPARγ transcriptional activity^[1]. Oleuropein induces apoptosis in breast cancer cells via the p53-dependent pathway and through the regulation of Bax and Bcl2 genes. Oleuropein also inhibits aromatase^[2].

IC₅₀ & Target

PPARγ

Aromatase

Apoptosis

Cellular Effect

Cell Line	Type	Value	Description	References
B16-F1	IC₅₀	283.9 μM Compound: 1	Antiproliferative activity against mouse B16-F1 cells after 72 hrs by MTT reduction assay Antiproliferative activity against mouse B16-F1 cells after 72 hrs by MTT reduction assay	[PMID: 28551177]
HCT-116	IC₅₀	181.86 μM Compound: 1	Antiproliferative activity against human HCT116 cells after 72 hrs by MTT reduction assay Antiproliferative activity against human HCT116 cells after 72 hrs by MTT reduction assay	[PMID: 28551177]
HL-60	IC₅₀	54.26 μM Compound: 1	Antiproliferative activity against human HL60 cells after 72 hrs by MTT reduction assay Antiproliferative activity against human HL60 cells after 72 hrs by MTT reduction assay	[PMID: 28551177]
HeLa	IC₅₀	265.28 μM Compound: 1	Antiproliferative activity against human HeLa cells after 72 hrs by MTT reduction assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT reduction assay	[PMID: 28551177]
K562	IC₅₀	64.73 μM Compound: 1	Antiproliferative activity against human K562 cells after 72 hrs by MTT reduction assay Antiproliferative activity against human K562 cells after 72 hrs by MTT reduction assay	[PMID: 28551177]
MCF7	IC₅₀	161.57 μM Compound: 1	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT reduction assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTT reduction assay	[PMID: 28551177]
RAW264.7	IC₅₀	> 80 μM Compound: 11	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess reagent-based assay Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess reagent-based assay	[PMID: 32936639]
SK-BR-3	IC₅₀	160.44 μM Compound: 1	Antiproliferative activity against human SKBR3 cells after 72 hrs by MTT reduction assay Antiproliferative activity against human SKBR3 cells after 72 hrs by MTT reduction assay	[PMID: 28551177]

In Vitro

Aromatase, a cytochrome P450 enzyme, is an important pharmacological target in breast cancer therapy^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

540.51

Formula

C₂₅H₃₂O₁₃

CAS No.

32619-42-4

Appearance

Solid

Color

White to light yellow

SMILES

OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@](O[C@@H]2OC=C(C(OC)=O)[C@@H](CC(OCCC3=CC=C(O)C(O)=C3)=O)/C2=C\C)([H])O1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (231.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 20 mg/mL (37.00 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8501 mL	9.2505 mL	18.5010 mL
5 mM	0.3700 mL	1.8501 mL	3.7002 mL
10 mM	0.1850 mL	0.9251 mL	1.8501 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (277 KB) SDS (396 KB)

COA (253 KB) HNMR (315 KB) RP-HPLC (246 KB) MS (205 KB)

Handling Instructions (2659 KB)

References

[1]. Svobodova M, et al. Oleuropein as an inhibitor of peroxisome proliferator-activated receptor gamma. Genes Nutr. 2014 Jan;9(1):376. [Content Brief]

[2]. Gorzynik-Debicka M, et al. Potential Health Benefits of Olive Oil and Plant Polyphenols. Int J Mol Sci. 2018 Feb 28;19(3). pii: E686. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.8501 mL	9.2505 mL	18.5010 mL	46.2526 mL
	5 mM	0.3700 mL	1.8501 mL	3.7002 mL	9.2505 mL
	10 mM	0.1850 mL	0.9251 mL	1.8501 mL	4.6253 mL
	15 mM	0.1233 mL	0.6167 mL	1.2334 mL	3.0835 mL
	20 mM	0.0925 mL	0.4625 mL	0.9251 mL	2.3126 mL
	25 mM	0.0740 mL	0.3700 mL	0.7400 mL	1.8501 mL
	30 mM	0.0617 mL	0.3084 mL	0.6167 mL	1.5418 mL
DMSO	40 mM	0.0463 mL	0.2313 mL	0.4625 mL	1.1563 mL
	50 mM	0.0370 mL	0.1850 mL	0.3700 mL	0.9251 mL
	60 mM	0.0308 mL	0.1542 mL	0.3084 mL	0.7709 mL
	80 mM	0.0231 mL	0.1156 mL	0.2313 mL	0.5782 mL
	100 mM	0.0185 mL	0.0925 mL	0.1850 mL	0.4625 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Oleuropein Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Oleuropein

Customer Review

Other Forms of Oleuropein:

Oleuropein Related Antibodies

9 Publications Citing Use of MCE Oleuropein

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Cytochrome P450 Isoform Specific Products:

View All PPAR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Oleuropein Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Oleuropein Related Classifications

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Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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