2. Immunology/Inflammation
  3. NO Synthase
  4. Physalin O

Physalin O is a physalin that can be isolated from Physalis angulata. Physalin O shows cytotoxicity to Hep G2 and MCF-7 cancer cells with IC50 values of 31.1 and 11.4 µM, respectively. Physalin O inhibits the production of nitric oxide (NO) and shows anti-inflammatory activities.

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Physalin O

Physalin O Chemical Structure

CAS No. : 120849-18-5

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Description

Physalin O is a physalin that can be isolated from Physalis angulata. Physalin O shows cytotoxicity to Hep G2 and MCF-7 cancer cells with IC50 values of 31.1 and 11.4 µM, respectively. Physalin O inhibits the production of nitric oxide (NO) and shows anti-inflammatory activities[1][2].

Cellular Effect
Cell Line Type Value Description References
HeLa IC50
>50 μM
Compound: PC-2
Inhibition of TNFalpha-induced IkappaBalpha (unknown origin) phosphorylation expressed in human HeLa cells pretreated 30 mins before TNFalpha addition measured up to 120 mins by ELISA in presence of proteasome inhibitor MG-132
Inhibition of TNFalpha-induced IkappaBalpha (unknown origin) phosphorylation expressed in human HeLa cells pretreated 30 mins before TNFalpha addition measured up to 120 mins by ELISA in presence of proteasome inhibitor MG-132
[PMID: 24900739]
HeLa IC50
>50 μM
Compound: PC-2
Inhibition of TNFalpha-induced NF-kappaB activation (unknown origin) expressed in human HeLa cells pretreated 30 mins before TNFalpha addition after 7 hrs by luciferase reporter gene assay
Inhibition of TNFalpha-induced NF-kappaB activation (unknown origin) expressed in human HeLa cells pretreated 30 mins before TNFalpha addition after 7 hrs by luciferase reporter gene assay
[PMID: 24900739]
RAW264.7 IC50
17.11 μM
Compound: 12
Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite formation
Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite formation
[PMID: 18348534]
Molecular Weight

528.55

Formula

C28H32O10
CAS No.
Appearance

Powder

Color

white

SMILES

C[C@@]12[C@]34[C@](C([C@](O3)([C@@]5([H])[C@]([C@@]6(C(CC=CC6=O)=C[C@H]5O)C)([H])CC[C@@]4(C(O2)=O)O)O)=O)([H])[C@@]7(C[C@H]1OC([C@H]7C)=O)C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (47.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8920 mL 9.4598 mL 18.9197 mL
5 mM 0.3784 mL 1.8920 mL 3.7839 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8920 mL 9.4598 mL 18.9197 mL 47.2992 mL
5 mM 0.3784 mL 1.8920 mL 3.7839 mL 9.4598 mL
10 mM 0.1892 mL 0.9460 mL 1.8920 mL 4.7299 mL
15 mM 0.1261 mL 0.6307 mL 1.2613 mL 3.1533 mL
20 mM 0.0946 mL 0.4730 mL 0.9460 mL 2.3650 mL
25 mM 0.0757 mL 0.3784 mL 0.7568 mL 1.8920 mL
30 mM 0.0631 mL 0.3153 mL 0.6307 mL 1.5766 mL
40 mM 0.0473 mL 0.2365 mL 0.4730 mL 1.1825 mL
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Physalin O Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Physalin O120849-18-5NO SynthaseNitric oxide synthasesNOSphysalinNOanti-inflammatoryHep G2MCF-7Inhibitorinhibitorinhibit

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