PROTAC BTK Degrader-2
Based on 1 Customer Validation
PROTAC BTK Degrader-2 is a potent BTK PROTAC degrader. PROTAC BTK Degrader-2 effectively reduces BTK protein levels.
(Pink: Btk ligand (HY-168314); Blue: Cereblon ligand (HY-14658); Black: linker (HY-130537)).
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 2250382-66-0
- Formula: C47H54F2N8O13
- Molecular Weight:976.97
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All PROTACs Isoforms
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Ramos | DC50 |
1.1 nM
Compound: 31
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Protac activity at CRBN/BTK in human Ramos cells assessed as induction of BTK protein degradation incubated for 24 hrs by Western blot analysis
Protac activity at CRBN/BTK in human Ramos cells assessed as induction of BTK protein degradation incubated for 24 hrs by Western blot analysis
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[PMID: 31047748] |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:male Wistar Han rats[1]
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Dosage:0, 0.35, 35, and 175 mg/kg
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Administration:subcutaneous injection
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Result:Degraded BTK in a dose-dependent and tissue-biased manner.
Chemical Information
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CAS No. 2250382-66-0
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Appearance Solid
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Molecular Weight 976.97
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Formula C47H54F2N8O13
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Color Light yellow to yellow
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SMILES
O=C(N1C[C@H](N2N=C(C3=CC=C(OC4=CC=C(F)C=C4F)C=C3)C(C(N)=O)=C2N)CCC1)OCCOCCOCCOCCOCCOCCNC5=CC=CC(C(N6C(CC7)C(NC7=O)=O)=O)=C5C6=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (102.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (5.12 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.0236 mL | 5.1179 mL | 10.2357 mL | 25.5893 mL |
| 5 mM | 0.2047 mL | 1.0236 mL | 2.0471 mL | 5.1179 mL | |
| 10 mM | 0.1024 mL | 0.5118 mL | 1.0236 mL | 2.5589 mL | |
| 15 mM | 0.0682 mL | 0.3412 mL | 0.6824 mL | 1.7060 mL | |
| 20 mM | 0.0512 mL | 0.2559 mL | 0.5118 mL | 1.2795 mL | |
| 25 mM | 0.0409 mL | 0.2047 mL | 0.4094 mL | 1.0236 mL | |
| 30 mM | 0.0341 mL | 0.1706 mL | 0.3412 mL | 0.8530 mL | |
| 40 mM | 0.0256 mL | 0.1279 mL | 0.2559 mL | 0.6397 mL | |
| 50 mM | 0.0205 mL | 0.1024 mL | 0.2047 mL | 0.5118 mL | |
| 60 mM | 0.0171 mL | 0.0853 mL | 0.1706 mL | 0.4265 mL | |
| 80 mM | 0.0128 mL | 0.0640 mL | 0.1279 mL | 0.3199 mL | |
| 100 mM | 0.0102 mL | 0.0512 mL | 0.1024 mL | 0.2559 mL |
PROTAC BTK Degrader-2 Related Classifications
HY-122828 Related Classifications
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