PVP-037.2
Based on 1 Customer Validation
PVP-037.2 is a TLR7/8 agonist. PVP-037.2 can serve as an adjuvant to enhance vaccine-induced TH1 type immune responses, increasing the production of antigen-specific antibodies IgG1 and IgG2c.
For research use only. We do not sell to patients.
- Purity: 98.40%
- CAS No.: 3049666-32-9
- Formula: C23H20F3N5O
- Molecular Weight:439.43
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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TLR7 |
TLR8 |
PVP-037.2 (0.1-100 μM, 24 h) induces concentration-dependent production of TNF and IL-1β in human elder peripheral blood mononuclear cells (PBMCs)[1]. PVP-037.2 ((100 μM, 20-24 h) demonstrates a TLR7/8-dependent immunomodulatory profile[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:WT C57BL/6J mice
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Dosage:10 nmol per mouse
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Administration:Intravenous injection (i.v.), single injection
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Result:Significantly enhanced total IgG and IgG2c class-switched antibody responses (IgG titers measured on day 42).
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Animal Model:C57BL/6J mice (6 to 8 weeks old, stimulated with the trivalent recombinant rHA influenza vaccine Flublok and the SARS-CoV-2 spike protein)
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Dosage:100 nmol per mouse
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Administration:Intravenous injection (i.v.), single injection
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Result:Resulted that the production of antigen-specific IgG1 and IgG2c was significantly enhanced in C57BL/6J mice stimulated with the trivalent recombinant rHA influenza vaccine Flublok (IgG titers measured on day 28) and the SARS-CoV-2 spike protein (IgG titers measured on day 42).
Chemical Information
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CAS No. 3049666-32-9
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Appearance Solid
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Molecular Weight 439.43
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Formula C23H20F3N5O
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Color White to yellow
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SMILES
CC1=CN2C=C(N=C2N=C1)C3=CC(NC(C4=C(C=C(C=C4)CN)C(F)(F)F)=O)=C(C=C3)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (227.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2757 mL | 11.3784 mL | 22.7568 mL | 56.8919 mL |
| 5 mM | 0.4551 mL | 2.2757 mL | 4.5514 mL | 11.3784 mL | |
| 10 mM | 0.2276 mL | 1.1378 mL | 2.2757 mL | 5.6892 mL | |
| 15 mM | 0.1517 mL | 0.7586 mL | 1.5171 mL | 3.7928 mL | |
| 20 mM | 0.1138 mL | 0.5689 mL | 1.1378 mL | 2.8446 mL | |
| 25 mM | 0.0910 mL | 0.4551 mL | 0.9103 mL | 2.2757 mL | |
| 30 mM | 0.0759 mL | 0.3793 mL | 0.7586 mL | 1.8964 mL | |
| 40 mM | 0.0569 mL | 0.2845 mL | 0.5689 mL | 1.4223 mL | |
| 50 mM | 0.0455 mL | 0.2276 mL | 0.4551 mL | 1.1378 mL | |
| 60 mM | 0.0379 mL | 0.1896 mL | 0.3793 mL | 0.9482 mL | |
| 80 mM | 0.0284 mL | 0.1422 mL | 0.2845 mL | 0.7111 mL | |
| 100 mM | 0.0228 mL | 0.1138 mL | 0.2276 mL | 0.5689 mL |
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