TAM470
Based on 1 Customer Validation
TAM470 is a novel cytolysin, inhibiting tubulin polymerization and microtubule depolymerization. TAM470 can be used in the synthesis of OMTX705 as payload molecule, OMTX705 is a novel FAP-targeting antibody-drug conjugates (ADCs) with antitumor activity.
For research use only. We do not sell to patients.
- Purity: 95.64%
- CAS No.: 1802498-63-0
- Formula: C41H67N7O6S
- Molecular Weight:786.08
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
TAM470 binds to β-tubulin and destroys α-β tubulin heterodimers, inhibits microtubule polymerization and depolymerization[1].
TAM470 (10 nM-100 μM, 5 days) exhibits cytotoxicity in wildtype HT1080 , FAP-expressing HT1080 and CAF07[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT1080 WT, HT1080-FAP, CAF
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Concentration:10 nM-100 μM
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Incubation Time:5 days
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Result:Reduced the cell density.
Chemical Information
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CAS No. 1802498-63-0
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Appearance Solid
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Molecular Weight 786.08
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Formula C41H67N7O6S
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Color White to off-white
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SMILES
O=C([C@@H]1N(CCCC1)C)N[C@@H]([C@@H](C)CC)C(N(CCC)[C@@H](C(C)C)C[C@H](C2=NC(C(N[C@H](C[C@H](C)C(NN)=O)CC3=CC=C(C=C3)O)=O)=CS2)OCCC)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (127.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (2.54 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2721 mL | 6.3607 mL | 12.7214 mL | 31.8034 mL |
| 5 mM | 0.2544 mL | 1.2721 mL | 2.5443 mL | 6.3607 mL | |
| 10 mM | 0.1272 mL | 0.6361 mL | 1.2721 mL | 3.1803 mL | |
| 15 mM | 0.0848 mL | 0.4240 mL | 0.8481 mL | 2.1202 mL | |
| 20 mM | 0.0636 mL | 0.3180 mL | 0.6361 mL | 1.5902 mL | |
| 25 mM | 0.0509 mL | 0.2544 mL | 0.5089 mL | 1.2721 mL | |
| 30 mM | 0.0424 mL | 0.2120 mL | 0.4240 mL | 1.0601 mL | |
| 40 mM | 0.0318 mL | 0.1590 mL | 0.3180 mL | 0.7951 mL | |
| 50 mM | 0.0254 mL | 0.1272 mL | 0.2544 mL | 0.6361 mL | |
| 60 mM | 0.0212 mL | 0.1060 mL | 0.2120 mL | 0.5301 mL | |
| 80 mM | 0.0159 mL | 0.0795 mL | 0.1590 mL | 0.3975 mL | |
| 100 mM | 0.0127 mL | 0.0636 mL | 0.1272 mL | 0.3180 mL |
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