TCB-32 hydrochloride

Cat. No.: HY-176862A: Data Sheet
SDS
Handling Instructions
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TCB-32 (Compound I-1) hydrochloride is a FGFR1 agonist with an EC₅₀ of 0.88  μM. TCB-32 hydrochloride significantly increases cell proliferation through activating FGFR1 signaling pathway as bFGF and its downstream ERK1/2 with excellent thermal stability. TCB-32 hydrochloride can replace bFGF in serum-free cell culture media. TCB-32 hydrochloride can be used for tissue repair and wound healing related diseases like psoriasis and eczema research.

For research use only. We do not sell to patients.

TCB-32 hydrochloride Chemical Structure

CAS No. : 169764-95-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Other Forms of TCB-32 hydrochloride:

TCB-32 Get quote

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•Related Screening Libraries:

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View All ERK Isoform Specific Products:

View All Isoforms

ERK ERK1 ERK2 ERK5 ERK8

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]^[2]

FGFR1

0.88 μM (EC₅₀)

ERK1

ERK2

Molecular Weight

854.62

Formula

C₃₂H₄₄Cl₄N₁₈O₂

CAS No.

169764-95-8

Appearance

Solid

SMILES

O=C(C1=CC=CC(C(NC2=CC(/C(C)=N/NC(N)=N)=CC(/C(C)=N/NC(N)=N)=C2)=O)=C1)NC3=CC(/C(C)=N/NC(N)=N)=CC(/C(C)=N/NC(N)=N)=C3.Cl.Cl.Cl.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (117.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1701 mL	5.8506 mL	11.7011 mL
5 mM	0.2340 mL	1.1701 mL	2.3402 mL
10 mM	0.1170 mL	0.5851 mL	1.1701 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Data Sheet (273 KB) SDS (252 KB) Handling Instructions (2659 KB)

References

[1]. FEOFANOV M, et al. Compounds for activation of fgfr1 signaling. WO2025045826A1. 2025-03-06.

[2]. Feofanov M, et al. Discovery and optimization of a guanylhydrazone-based small molecule to replace bFGF for cell culture applications. Biochem Biophys Rep. 2025 Jul 21;43:102167. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1701 mL	5.8506 mL	11.7011 mL	29.2528 mL
	5 mM	0.2340 mL	1.1701 mL	2.3402 mL	5.8506 mL
	10 mM	0.1170 mL	0.5851 mL	1.1701 mL	2.9253 mL
	15 mM	0.0780 mL	0.3900 mL	0.7801 mL	1.9502 mL
	20 mM	0.0585 mL	0.2925 mL	0.5851 mL	1.4626 mL
	25 mM	0.0468 mL	0.2340 mL	0.4680 mL	1.1701 mL
	30 mM	0.0390 mL	0.1950 mL	0.3900 mL	0.9751 mL
	40 mM	0.0293 mL	0.1463 mL	0.2925 mL	0.7313 mL
	50 mM	0.0234 mL	0.1170 mL	0.2340 mL	0.5851 mL
	60 mM	0.0195 mL	0.0975 mL	0.1950 mL	0.4875 mL
	80 mM	0.0146 mL	0.0731 mL	0.1463 mL	0.3657 mL
	100 mM	0.0117 mL	0.0585 mL	0.1170 mL	0.2925 mL