Thailanstatin C
Thailanstatin C is a pre-mRNA splicing inhibitor (IC50= 6.84 μM) and antiproliferative agent from Burkholderia thailandensis MSMB43.
For research use only. We do not sell to patients.
- CAS No.: 1426953-24-3
- Formula: C30H46ClNO9
- Molecular Weight:600.14
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| DU-145 | GI50 |
2.98 nM
Compound: 3
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Antiproliferative activity against human DU145 cells assessed as growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human DU145 cells assessed as growth inhibition measured after 48 hrs by MTT assay
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[PMID: 23517093] |
| HEK293 | IC50 |
6.84 μM
Compound: 3
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Inhibition of Pre-mRNA splicing in human HEK293 cells using p32-labeled CDC14-15 pre-mRNA by exonjunction complex (EJC) immunoprecipitation assay
Inhibition of Pre-mRNA splicing in human HEK293 cells using p32-labeled CDC14-15 pre-mRNA by exonjunction complex (EJC) immunoprecipitation assay
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[PMID: 23517093] |
| HEK-293T | IC50 |
64.7 nM
Compound: 7
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Cytotoxicity against HEK293T cells assessed as inhibition in cell growth
Cytotoxicity against HEK293T cells assessed as inhibition in cell growth
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[PMID: 33974423] |
| MDA-MB-231 | GI50 |
8.82 nM
Compound: 3
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Antiproliferative activity against human MDA-MB-231 cells assessed as growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human MDA-MB-231 cells assessed as growth inhibition measured after 48 hrs by MTT assay
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[PMID: 23517093] |
| MES-SA | IC50 |
136.7 nM
Compound: 7
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Cytotoxicity against human MES-SA cells assessed as inhibition in cell growth
Cytotoxicity against human MES-SA cells assessed as inhibition in cell growth
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[PMID: 33974423] |
| MES-SA/Dx5 | IC50 |
298.5 nM
Compound: 7
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Cytotoxicity against human MES-SA/Dx5 cells assessed as inhibition in cell growth
Cytotoxicity against human MES-SA/Dx5 cells assessed as inhibition in cell growth
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[PMID: 33974423] |
| SK-OV-3 | GI50 |
5.57 nM
Compound: 3
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Antiproliferative activity against human SKOV3 cells assessed as growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human SKOV3 cells assessed as growth inhibition measured after 48 hrs by MTT assay
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[PMID: 23517093] |
In Vitro
Chemical Information
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CAS No. 1426953-24-3
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Molecular Weight 600.14
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Formula C30H46ClNO9
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SMILES
C[C@@H](C[C@H]([C@H](O1)C)NC(/C=C\[C@H](C)OC(C(C)C)=O)=O)[C@@H]1C/C=C(C)/C=C/[C@@H]2[C@H]([C@@](O)(C[C@H](O2)CC(O)=O)CCl)O
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Structure Classification
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Initial Source
Burkholderia thailandensis
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Thailanstatin C Related Classifications
HY-139103 Related Classifications
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