Yatein
Yatein is a lignan isolated from A. chilensis, with antiproliferative activity. Yatein suppresses herpes simplex virus type 1 (HSV-1 ) replication by interruption the immediate-early gene expression.
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- CAS No.: 40456-50-6
- Formule: C22H24O7
- Masse moléculaire:400.42
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
IC50 & Target
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HSV-1 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A-431 | ED50 |
>20 μg/mL
Compound: 5
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Cytotoxicity against human A431 cells
Cytotoxicity against human A431 cells
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[PMID: 8277312] |
| BC1 cell line | ED50 |
0.05 μg/mL
Compound: 5
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Cytotoxicity against human BC1 cells
Cytotoxicity against human BC1 cells
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[PMID: 8277312] |
| Col2 | ED50 |
0.08 μg/mL
Compound: 5
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Cytotoxicity against human Col2 cells
Cytotoxicity against human Col2 cells
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[PMID: 8277312] |
| HT | ED50 |
0.07 μg/mL
Compound: 5
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Cytotoxicity against human HT cells
Cytotoxicity against human HT cells
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[PMID: 8277312] |
| KB | ED50 |
0.08 μg/mL
Compound: 5
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 8277312] |
| KB-V1 | ED50 |
0.06 μg/mL
Compound: 5
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Cytotoxicity against human KBV1 cells
Cytotoxicity against human KBV1 cells
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[PMID: 8277312] |
| LNCaP | ED50 |
0.16 μg/mL
Compound: 5
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Cytotoxicity against human LNCAP cells
Cytotoxicity against human LNCAP cells
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[PMID: 8277312] |
| Lu1 | ED50 |
0.1 μg/mL
Compound: 5
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Cytotoxicity against human Lu1 cells
Cytotoxicity against human Lu1 cells
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[PMID: 8277312] |
| P388 | ED50 |
0.4 μg/mL
Compound: 5
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Cytotoxicity in mouse P388 cells
Cytotoxicity in mouse P388 cells
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[PMID: 8277312] |
| U-373MG ATCC | ED50 |
0.3 μg/mL
Compound: 5
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Cytotoxicity against human U373 cells
Cytotoxicity against human U373 cells
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[PMID: 8277312] |
| ZR-75-1 | ED50 |
0.5 μg/mL
Compound: 5
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Cytotoxicity against human ZR-75-1 cells
Cytotoxicity against human ZR-75-1 cells
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[PMID: 8277312] |
In Vitro
Yatein induces cell-cycle arrest at G2/M phase (5 μM; 24 hours) and enhances G2/M phase-related protein expression (5 μM; 6-12 hours) in human lung adenocarcinoma A549 and CL1-5 cells[3].
Yatein (5 μM; 6-12 hours) induces DNA damage through activation of the ATM/ATR pathway in human A549 and CL1-5 cells[3].
Yatein (5 μM; 6 hours) affects microtubule dynamics by inhibiting tubulin polymerization[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:A549 cells, CL1-5 cells
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Concentration:1.25 μM , 2.5 μM , 5 μM
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Incubation Time:24 hours
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Result:Induced cell-cycle arrest at G2/M phase in both cell lines.
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Cell Line:A549 cells, CL1-5 cells
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Concentration:5 μM
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Incubation Time:6 hours, 12 hours
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Result:Upregulated the expression of cyclin B1, but not Cdc2 and Cdc25c, induced Cdc2 phosphorylation.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male NOD/SCID mice (6-8 weeks), with A549 cells xenograft[3]
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Dosage:20 mg/kg
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Administration:Intraperitoneal injection, five times per week, for 42 days
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Result:Significantly slowed tumor growth and moderately increased both cyclin B1 expression and Cdc2 phosphorylation.
Chemical Information
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CAS No. 40456-50-6
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Appearance Oil
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Masse moléculaire 400.42
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Formule C22H24O7
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Color Colorless to light yellow
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SMILES
O=C1OC[C@H](CC2=CC=C(OCO3)C3=C2)[C@H]1CC4=CC(OC)=C(OC)C(OC)=C4
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Protocole
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RT-PCR
Reverse transcription technology uses RNA as a template to synthesize DNA. RT-PCR is simple, specific and sensitive, and can be used to detect gene expression levels and expression differences in cells; detect RNA virus content; clone cDNA sequences of specific genes.
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Real Time qPCR (Q-PCR)
Real-time quantitative PCR (qPCR) quantifies an amplifiable nucleic-acid target by monitoring fluorescence during PCR cycling rather than measuring product only after amplification. The increase in fluorescence tracks accumulation of PCR product, and the quantification cycle (Cq; historically also Ct/CP) is related to the initial amount of target: samples containing more starting target generally reach the defined fluorescence threshold in fewer cycles.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
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Fiche technique (282 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Donoso-Fierro C, et al. Antiproliferative activity of yatein isolated from Austrocedrus chilensis against murine myeloma cells: cytological studies and chemical investigations. Pharm Biol. 2015 Mar;53(3):378-85. [Content Brief]
[2]. Kuo YC, et al. Yatein from Chamaecyparis obtusa suppresses herpes simplex virus type 1 replication in HeLa cells by interruption the immediate-early gene expression. Antiviral Res. 2006 Jul;70(3):112-20. [Content Brief]
[3]. Shang-Tse Ho, et al. Molecular Mechanisms Underlying Yatein-Induced Cell-Cycle Arrest and Microtubule Destabilization in Human Lung Adenocarcinoma Cells. Cancers (Basel). 2019 Sep; 11(9): 1384. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Yatein Related Classifications
HY-N1060 Related Classifications
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