ZEB85 

ZEB85 is a human monoclonal antibody (mAb) targeting TrkB. ZEB85 activates TrkB and its downstream cascades, including the ERK, PLCγ, AKT, MAPK signaling pathways and cFOS expression, and enhances neuronal activity. ZEB85 prevents β-amyloid toxicity in cultured hippocampal neurons. ZEB85 is applicable to the research of Alzheimer's disease (AD)^[1][2].

scFv-hFc

Human

ZEB85 (10-100 nM; 4 days) increases neurite complexity in developing primary mouse hippocampal neurons, including neurite intersections, branch points and total length^[1].
ZEB85 (100 nM; 21 days) fully rescues the reduced dendritic complexity of BDNF-deficient primary mouse hippocampal PV+ interneurons and increases the dendritic complexity of BDNF-sufficient PV+ interneurons^[1].
ZEB85 (10 nM; 24 h) shifts the dendritic spine type distribution of mature primary mouse hippocampal neurons toward more mushroom-type and fewer thin-type spines^[1].
ZEB85 (100 nM; 21 h) completely reverses Aβ_1-42-induced dendritic spine loss and altered spine type distribution in mature primary mouse hippocampal neurons^[1].
ZEB85 (100 nM; 15 min-2 h) activates the downstream TrkB signaling pathway in mature primary mouse hippocampal neurons and upregulates the expression of cFOS and pERK, whereas 10 nM ZEB85 fails to significantly activate this signaling pathway^[1].
ZEB85 (100 nM; 24 h) increases the amplitude of spontaneous calcium transients, a marker of neuronal activity, in mature primary mouse hippocampal neurons^[1].
ZEB85 (100 nM; 5-6 days) rescues the LTP induction deficit in hippocampal organotypic cultures of BDNF^hetKO mouse brain slices, increases the success rate of LTP induction to 60%, and promotes stable and long-lasting synaptic potentiation^[1].
ZEB85 (100 nM; 5-30 min) activates the canonical TrkB signaling pathway (phosphorylation of PLCγ, AKT, and ERK) in CHO-NFAT human TrkB reporter cells, with a magnitude and time course of effect similar to that of BDNF^[2].
ZEB85 potently induces TrkB phosphorylation in γ-aminobutyric acidergic neurons derived from H9 human embryonic stem cells, with an EC₅₀ of 470 pM^[2].
ZEB85 (50 μg/mL; 15-240 min) activates the phosphorylation of TrkB in cortical neurons of embryonic day 21 mice^[2].
ZEB85 (50 μg/mL; 3 days) significantly enhances dendritic growth of retinal ganglion cells in cultured adult mouse retinal explants^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4915  [NCBI]

Q16620

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

104.52 kDa

Liquid

Colorless to off-white

[ZEB85]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Flow cytometric analysis of 1X10⁶ Jurkat cells labeling TrkB with ZEB85 (HY-P991413, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 for an hour at 4℃. AF488-conjugated Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa, Isotype Control (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Tacke C, et al. Actions of the TrkB Agonist Antibody ZEB85 in Regulating the Architecture and Synaptic Plasticity in Hippocampal Neurons. Front Mol Neurosci. 2022;15:945348. Published 2022 Jun 30.  [Content Brief]

  [2]. Merkouris S, et al. Fully human agonist antibodies to TrkB using autocrine cell-based selection from a combinatorial antibody library. Proc Natl Acad Sci U S A. 2018;115(30):E7023-E7032.  [Content Brief]