Zuclomiphene
Based on 1 publication(s) in Google Scholar
Zuclomiphene is a cis isomer of Clomiphene citrate (HY-B0463). Zuclomiphene is orally active and has an antiestrogenic effect. Zuclomiphene can reduce cholesterol levels. Zuclomiphene can be for the researches of endocrinology and metabolic disease.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.62%
- CAS. Nr.: 15690-55-8
- Formel: C26H28ClNO
- Molecular Weight:405.96
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Speicherung:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Zuclomiphene
More
Biologische Aktivität
Beschreibung
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HT-1080 | IC50 |
37.86 μM
Compound: 76
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Inhibition of IDH1 R132H mutant in human HT-1080 cells assessed as suppression of 2-HG production incubated for 48 hrs by LC-MS analysis
Inhibition of IDH1 R132H mutant in human HT-1080 cells assessed as suppression of 2-HG production incubated for 48 hrs by LC-MS analysis
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[PMID: 29847930] |
In Vivo
Zuclomiphene (30 mg/kg, i.p., twice a week) reduces cholesterol content in rats[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male mice[1]
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Dosage:4 and 40 mg/kg
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Administration:Orally gavage
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Result:Reduced leydig cells surrounding the tubules in size and numbers.
Showed testicular degeneration and an absence of sperm in the seminiferous tubules.
Reduced kidney weights and body weight.
Reduced levels of LH, FSH and testosterone.
Chemical Information
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CAS. Nr. 15690-55-8
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Appearance Solid
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Molecular Weight 405.96
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Formel C26H28ClNO
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SMILES
Cl/C(C1=CC=CC=C1)=C(C2=CC=C(OCCN(CC)CC)C=C2)/C3=CC=CC=C3
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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J Biol Chem
TFEB agonist clomiphene citrate activates the autophagy-lysosomal pathway and ameliorates Alzheimer's disease symptoms in mice. [Abstract]2024 Dec;300(12):107929. PMID: 39454957
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 33.33 mg/mL (82.10 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.33 mg/mL (8.20 mM); Clear solution
This protocol yields a clear solution of ≥ 3.33 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (33.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 3.33 mg/mL (8.20 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 3.33 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (33.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
Verweise
[1]. Fontenot GK, et al. Differential effects of isomers of clomiphene citrate on reproductive tissues in male mice. BJU Int. 2016 Feb;117(2):344-50. [Content Brief]
[2]. Ramsey RB, et al. Effects of zuclomiphene in combination with triparanol and ay-9944 on developing rat CNS morphology and biochemistry. Acta Neuropathol. 1977 Jul 15;39(1):97-100. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4633 mL | 12.3165 mL | 24.6330 mL | 61.5824 mL |
| 5 mM | 0.4927 mL | 2.4633 mL | 4.9266 mL | 12.3165 mL | |
| 10 mM | 0.2463 mL | 1.2316 mL | 2.4633 mL | 6.1582 mL | |
| 15 mM | 0.1642 mL | 0.8211 mL | 1.6422 mL | 4.1055 mL | |
| 20 mM | 0.1232 mL | 0.6158 mL | 1.2316 mL | 3.0791 mL | |
| 25 mM | 0.0985 mL | 0.4927 mL | 0.9853 mL | 2.4633 mL | |
| 30 mM | 0.0821 mL | 0.4105 mL | 0.8211 mL | 2.0527 mL | |
| 40 mM | 0.0616 mL | 0.3079 mL | 0.6158 mL | 1.5396 mL | |
| 50 mM | 0.0493 mL | 0.2463 mL | 0.4927 mL | 1.2316 mL | |
| 60 mM | 0.0411 mL | 0.2053 mL | 0.4105 mL | 1.0264 mL | |
| 80 mM | 0.0308 mL | 0.1540 mL | 0.3079 mL | 0.7698 mL |
Zuclomiphene Related Classifications
HY-B1617 Related Classifications
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