α-Synuclein aggregate binder 1
Based on 1 Customer Validation
α-Synuclein aggregate binder 1 (Compound C05-05) is a specific binder for α-synuclein aggregates and can inhibit α-synuclein aggregation. α-Synuclein aggregate binder 1 can be used as a fluorescent probe (excitation wavelength 900 nm, detection wavelength 500-550 nm) for optical imaging, and can also inhibit α-synuclein fibril formation by blocking the aggregation process. α-Synuclein aggregate binder 1 can be used for positron emission tomography (PET) imaging after being labeled with 18F. α-Synuclein aggregate binder 1 can be used for visual diagnosis of brain lesions and mechanism research of neurodegenerative diseases such as Parkinson's disease and Lewy body dementia.
For research use only. We do not sell to patients.
- Purity: 98.11%
- CAS No.: 1565797-17-2
- Formula: C20H18FN3O2S
- Molecular Weight:383.44
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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α-synuclein Aggregation |
Optical imaging of mouse brain:
α-Synuclein aggregate binder 1 (C05-05) (30μM; 30 min) specifically binds to the phosphorylated α-synuclein lesion area in the brain slices of mice inoculated with α-synuclein fibrils, and the fluorescence signal overlaps with the anti-phosphorylated α-synuclein antibody staining area[1].
PET imaging experiment:
18F-labeled C05-05 showed appropriate brain penetration and clearance rate in normal mouse brains, and specifically aggregated in the striatum of α-synuclein fibril-inoculated mouse model[1].
α-synuclein aggregation inhibition experiment: C05-05 (20 μM/200 μM; 24 h shaking culture) inhibited the formation of α-synuclein aggregates in a dose-dependent manner, with an inhibition rate of 60% at 200 μM[1].
Operational steps and methods for different experimental purposes
(1) Fluorescence analysis of human/mouse brain slices[1]:
20-6 μm thick brain slices were incubated with 30 μM C05-05 in 50% (human brain) or 20% (mouse brain) ethanol solution at room temperature for 30 minutes.
Washed with ethanol and ultrapure water in turn, and the slices were sealed.
After sealing, the fluorescence signal of the lesion area was observed by Two-photon laser scanning microscope (excitation wavelength 900 nm, detection wavelength 500-550 nm).
(2) PET imaging of mice[1]:
Anesthetized mice were intravenously injected with 18F-C05-05 (24.3±0.7MBq) and immediately scanned for 90-120 min.
Images were reconstructed using Fourier reconstruction and maximum a posteriori probability method, and the distribution of radioactivity in the striatum and midbrain was analyzed.
(3) α-synuclein aggregation inhibition experiment[1]:
C05-05 (final concentration 20 μM or 200 μM) and protein aggregates were added to the A53T mutant α-synuclein monomer solution (1 mg/mL).
Incubate at 37°C with shaking for 24 h, and then add sarcosine for treatment.
After ultracentrifugation, insoluble aggregates were quantified by electrophoresis and CBB staining.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
α-Synuclein aggregate binder 1 (C05-05) (intravenous injection; detected after 90 minutes) is specifically enriched in the α-synuclein pathological area in the model mouse brain, with a detection wavelength of 500-550 nm, and the imaging contrast is better than that of the tau protein PET probe PBB3 (HY-114616)[1].
Experimental details[1]:
Model mice inoculated with α-synuclein fibrils for 6 weeks were injected with 50 μL of DMSO saline solution containing 0.05% C05-05 through the tail vein.
90 minutes later, sulforhodamine 101 was injected intraperitoneally.
Two-photon imaging was performed with an excitation light of 900 nm and detection wavelengths of 500-550 nm (C05-05) and 573-648 nm (control).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1565797-17-2
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Appearance Solid
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Molecular Weight 383.44
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Formula C20H18FN3O2S
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Color Light yellow to yellow
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SMILES
CNC1=NC=C(C=C1)C#C/C=C/C(SC2=C3)=NC2=CC=C3OCC(O)CF
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (260.80 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6080 mL | 13.0398 mL | 26.0797 mL | 65.1992 mL |
| 5 mM | 0.5216 mL | 2.6080 mL | 5.2159 mL | 13.0398 mL | |
| 10 mM | 0.2608 mL | 1.3040 mL | 2.6080 mL | 6.5199 mL | |
| 15 mM | 0.1739 mL | 0.8693 mL | 1.7386 mL | 4.3466 mL | |
| 20 mM | 0.1304 mL | 0.6520 mL | 1.3040 mL | 3.2600 mL | |
| 25 mM | 0.1043 mL | 0.5216 mL | 1.0432 mL | 2.6080 mL | |
| 30 mM | 0.0869 mL | 0.4347 mL | 0.8693 mL | 2.1733 mL | |
| 40 mM | 0.0652 mL | 0.3260 mL | 0.6520 mL | 1.6300 mL | |
| 50 mM | 0.0522 mL | 0.2608 mL | 0.5216 mL | 1.3040 mL | |
| 60 mM | 0.0435 mL | 0.2173 mL | 0.4347 mL | 1.0867 mL | |
| 80 mM | 0.0326 mL | 0.1630 mL | 0.3260 mL | 0.8150 mL | |
| 100 mM | 0.0261 mL | 0.1304 mL | 0.2608 mL | 0.6520 mL |
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