2. Induced Disease Models Products NF-κB MAPK/ERK Pathway Immunology/Inflammation
  3. Immunology and Inflammatory Disease Models NF-κB p38 MAPK COX
  4. Gastrointestinal Inflammation and Ulcer Models Hapten
  5. Inflammatory Bowel Disease (IBD) Models
  6. 2,4-Dinitrobenzenesulfonic acid sodium salt

2,4-Dinitrobenzenesulfonic acid sodium salt  (Synonyms: Sodium 2,4-dinitrobenzenesulfonate; DNBS sodium salt; DNBSO sodium salt)

Cat. No.: HY-W324435 Purity: 99.98%
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2,4-Dinitrobenzenesulfonic acid (DNBS; DNBSO) sodium salt (Sodium 2,4-dinitrobenzenesulfonate) is a classic colitis inducer that relies on activation of the NF-κB p65/COX-2/p38 pathway. As a hapten, 2,4-Dinitrobenzenesulfonic acid sodium salt stimulates the production of immune responses in colonic tissues, triggers oxidative stress and inflammatory reactions, and thereby leads to colonic injury. 2,4-Dinitrobenzenesulfonic acid sodium salt successfully induces models of colitis and ulcerative colitis in rats, causing pathological changes such as ulcers, edema, stenosis, shortening and organ adhesion in the distal colon, along with significant increases in the levels of inflammatory indicators and endoplasmic reticulum stress marker proteins. 2,4-Dinitrobenzenesulfonic acid sodium salt is widely used in studies on the mechanisms related to colitis and ulcerative colitis.

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2,4-Dinitrobenzenesulfonic acid sodium salt

2,4-Dinitrobenzenesulfonic acid sodium salt Chemical Structure

CAS No. : 885-62-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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2,4-Dinitrobenzenesulfonic acid sodium salt Related Antibodies

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Description

2,4-Dinitrobenzenesulfonic acid (DNBS; DNBSO) sodium salt (Sodium 2,4-dinitrobenzenesulfonate) is a classic colitis inducer that relies on activation of the NF-κB p65/COX-2/p38 pathway. As a hapten, 2,4-Dinitrobenzenesulfonic acid sodium salt stimulates the production of immune responses in colonic tissues, triggers oxidative stress and inflammatory reactions, and thereby leads to colonic injury. 2,4-Dinitrobenzenesulfonic acid sodium salt successfully induces models of colitis and ulcerative colitis in rats, causing pathological changes such as ulcers, edema, stenosis, shortening and organ adhesion in the distal colon, along with significant increases in the levels of inflammatory indicators and endoplasmic reticulum stress marker proteins. 2,4-Dinitrobenzenesulfonic acid sodium salt is widely used in studies on the mechanisms related to colitis and ulcerative colitis[1][2][3][4].

IC50 & Target

COX-2

 

In Vivo

Rectal administration of a single dose of 2,4-Dinitrobenzenesulfonic acid sodium salt (48.0 mg) induces severe colitis in male Sprague-Dawley rats, which is characterized by increased colonic injury scores, enhanced MPO activity, elevated levels of inflammatory mediators, and upregulated expression of endoplasmic reticulum (ER) stress marker proteins[2].
Sodium salt of 2,4-Dinitrobenzenesulfonic acid (1 mL/kg; intracolonic administration; single dose) induces severe ulcerative colitis in male Sprague-Dawley rats, which is characterized by increased levels of pro-inflammatory and oxidative stress markers, impaired antioxidant function, and extensive colonic tissue damage[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sprague-Dawley rat (male, 7-week-old, 250-260 g, colitis induced via rectal instillation of DNBS in 50% aqueous ethanol)[2]
Dosage: 48.0 mg
Administration: rectal instillation; single dose
Result: Induced severe colonic inflammation and mucosal damage, including tissue edema, stenosis, adhesion to neighboring organs, a colonic damage score of 4.7, myeloperoxidase activity of 4.3 units/g distal colon, elevated levels of inflammatory mediators (iNOS, COX-2, CINC-3 at 360 ng/g distal colon), and markedly increased ER stress marker proteins (ATF6, CHOP) in the distal colon.
Molecular Weight

270.15

Formula

C6H3N2NaO7S
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=[N+]([O-])C1=CC([N+]([O-])=O)=C(S(=O)(O[Na])=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (370.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7016 mL 18.5082 mL 37.0165 mL
5 mM 0.7403 mL 3.7016 mL 7.4033 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.98%

SDS (545 KB)

COA (254 KB) HNMR (248 KB) RP-HPLC (216 KB) MS (68 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.7016 mL 18.5082 mL 37.0165 mL 92.5412 mL
5 mM 0.7403 mL 3.7016 mL 7.4033 mL 18.5082 mL
10 mM 0.3702 mL 1.8508 mL 3.7016 mL 9.2541 mL
15 mM 0.2468 mL 1.2339 mL 2.4678 mL 6.1694 mL
20 mM 0.1851 mL 0.9254 mL 1.8508 mL 4.6271 mL
25 mM 0.1481 mL 0.7403 mL 1.4807 mL 3.7016 mL
30 mM 0.1234 mL 0.6169 mL 1.2339 mL 3.0847 mL
40 mM 0.0925 mL 0.4627 mL 0.9254 mL 2.3135 mL
50 mM 0.0740 mL 0.3702 mL 0.7403 mL 1.8508 mL
60 mM 0.0617 mL 0.3085 mL 0.6169 mL 1.5424 mL
80 mM 0.0463 mL 0.2314 mL 0.4627 mL 1.1568 mL
100 mM 0.0370 mL 0.1851 mL 0.3702 mL 0.9254 mL
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2,4-Dinitrobenzenesulfonic acid sodium salt Related Classifications

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Keywords:

2,4-Dinitrobenzenesulfonic acid885-62-1Sodium 2,4-dinitrobenzenesulfonateDNBSDNBSONF-κBp38 MAPKCOXNuclear factor-κBNuclear factor-kappaBCyclooxygenaseinflammatory mediatorsendoplasmic reticulum stress marker proteinsmale Sprague-Dawley ratsratscolon tissueulcerative colitiscolitisoxidative stressdistal coloninflammatory reactionsInhibitorinhibitorinhibit

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