2,4-Dinitrobenzenesulfonic acid sodium salt
Based on 1 Customer Validation
2,4-Dinitrobenzenesulfonic acid (DNBS; DNBSO) sodium salt (Sodium 2,4-dinitrobenzenesulfonate) is a classic colitis inducer that relies on activation of the NF-κB p65/COX-2/p38 pathway. As a hapten, 2,4-Dinitrobenzenesulfonic acid sodium salt stimulates the production of immune responses in colonic tissues, triggers oxidative stress and inflammatory reactions, and thereby leads to colonic injury. 2,4-Dinitrobenzenesulfonic acid sodium salt successfully induces models of colitis and ulcerative colitis in rats, causing pathological changes such as ulcers, edema, stenosis, shortening and organ adhesion in the distal colon, along with significant increases in the levels of inflammatory indicators and endoplasmic reticulum stress marker proteins. 2,4-Dinitrobenzenesulfonic acid sodium salt is widely used in studies on the mechanisms related to colitis and ulcerative colitis.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 885-62-1
- Formula: C6H3N2NaO7S
- Molecular Weight:270.15
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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COX-2 |
Sodium salt of 2,4-Dinitrobenzenesulfonic acid (1 mL/kg; intracolonic administration; single dose) induces severe ulcerative colitis in male Sprague-Dawley rats, which is characterized by increased levels of pro-inflammatory and oxidative stress markers, impaired antioxidant function, and extensive colonic tissue damage[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley rat (male, 7-week-old, 250-260 g, colitis induced via rectal instillation of DNBS in 50% aqueous ethanol)[2]
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Dosage:48.0 mg
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Administration:rectal instillation; single dose
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Result:Induced severe colonic inflammation and mucosal damage, including tissue edema, stenosis, adhesion to neighboring organs, a colonic damage score of 4.7, myeloperoxidase activity of 4.3 units/g distal colon, elevated levels of inflammatory mediators (iNOS, COX-2, CINC-3 at 360 ng/g distal colon), and markedly increased ER stress marker proteins (ATF6, CHOP) in the distal colon.
Chemical Information
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CAS No. 885-62-1
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Appearance Solid
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Molecular Weight 270.15
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Formula C6H3N2NaO7S
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Color Off-white to light yellow
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SMILES
O=[N+]([O-])C1=CC([N+]([O-])=O)=C(S(=O)(O[Na])=O)C=C1
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Synonyms
Sodium 2,4-dinitrobenzenesulfonate; DNBS sodium salt; DNBSO sodium salt
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (370.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (545 KB)
- English - EN (545 KB)
- Français - FR (545 KB)
- Deutsch - DE (545 KB)
- Norwegian - NO (545 KB)
- Español - ES (545 KB)
- Swedish - SV (545 KB)
- Italian - IT (545 KB)
- Korean - KR (545 KB)
- Portuguese - PT (545 KB)
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Handling Instructions (2659 KB)
References
[1]. Kang C, et al. Colon-Targeted Poly(ADP-ribose) Polymerase Inhibitors Synergize Therapeutic Effects of Mesalazine Against Rat Colitis Induced by 2,4-Dinitrobenzenesulfonic Acid. Pharmaceutics. 2024;16(12):1546. Published 2024 Dec 2. [Content Brief]
[2]. Kim S, et al. A Colon-Targeted Prodrug, 4-Phenylbutyric Acid-Glutamic Acid Conjugate, Ameliorates 2,4-Dinitrobenzenesulfonic Acid-Induced Colitis in Rats. Pharmaceutics. 2020;12(9):843. Published 2020 Sep 3. [Content Brief]
[3]. Shi G, et al. The amelioration of ulcerative colitis induced by Dinitrobenzenesulfonic acid with Radix Hedysari. J Food Biochem. 2020;44(10):e13421. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.7016 mL | 18.5082 mL | 37.0165 mL | 92.5412 mL |
| 5 mM | 0.7403 mL | 3.7016 mL | 7.4033 mL | 18.5082 mL | |
| 10 mM | 0.3702 mL | 1.8508 mL | 3.7016 mL | 9.2541 mL | |
| 15 mM | 0.2468 mL | 1.2339 mL | 2.4678 mL | 6.1694 mL | |
| 20 mM | 0.1851 mL | 0.9254 mL | 1.8508 mL | 4.6271 mL | |
| 25 mM | 0.1481 mL | 0.7403 mL | 1.4807 mL | 3.7016 mL | |
| 30 mM | 0.1234 mL | 0.6169 mL | 1.2339 mL | 3.0847 mL | |
| 40 mM | 0.0925 mL | 0.4627 mL | 0.9254 mL | 2.3135 mL | |
| 50 mM | 0.0740 mL | 0.3702 mL | 0.7403 mL | 1.8508 mL | |
| 60 mM | 0.0617 mL | 0.3085 mL | 0.6169 mL | 1.5424 mL | |
| 80 mM | 0.0463 mL | 0.2314 mL | 0.4627 mL | 1.1568 mL | |
| 100 mM | 0.0370 mL | 0.1851 mL | 0.3702 mL | 0.9254 mL |
2,4-Dinitrobenzenesulfonic acid sodium salt Related Classifications
HY-W324435 Related Classifications
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- 2,4-Dinitrobenzenesulfonic acid
- 885-62-1
- Sodium 2,4-dinitrobenzenesulfonate
- DNBS
- DNBSO
- NF-κB
- p38 MAPK
- COX
- inflammatory mediators
- endoplasmic reticulum stress marker proteins
- male Sprague-Dawley rats
- rats
- colon tissue
- ulcerative colitis
- colitis
- oxidative stress
- distal colon
- inflammatory reactions
- Inhibitor
- inhibitor
- inhibit