2'-O-Methylcytidine
Based on 1 Customer Validation
2'-O-Methylcytidine is an orally active 2'-substituted nucleoside as a inhibitor of HCV replication with antiviral activity. 2'-O-Methylcytidine inhibits RNA-dependent RNA polymerase (NS5B)-catalyzed RNA synthesis in vitro, in a manner that is competitive with substrate nucleoside triphosphate.
For research use only. We do not sell to patients.
- Purity: 99.71%
- CAS No.: 2140-72-9
- Formula: C10H15N3O5
- Molecular Weight:257.25
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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RNA Polymerase |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Huh-7 | CC50 |
>300 μM
Compound: 2'-methylcytidine
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Cytotoxicity against human Huh7 AVA5 cells
Cytotoxicity against human Huh7 AVA5 cells
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[PMID: 18082410] |
| Huh-7 | EC50 |
1.8 μM
Compound: 2'-methylcytidine
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Antiviral activity against Hepatitis C virus infected in human Huh7 AVA5 cells by replicon assay
Antiviral activity against Hepatitis C virus infected in human Huh7 AVA5 cells by replicon assay
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[PMID: 18082410] |
2'-O-Methylcytidine (0.01-100 μM, 24 h) inhibits HCV RNA replication with an IC50 value of 21 μM in HBI10A cells[1].
2'-O-Methylcytidine (2 μM, 23 h) yields very little triphosphate and suffers from extensive metabolism to species that are consistent with UTP and CTP in HBI10A cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Chronically infected chimpanzees[3]
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Dosage:16.6 mg/kg
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Administration:p.o., daily for 7 days
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Result:Resulted in 1.05 log10 copies/mL reduction of serum HCV RNA in chronically infected chimpanzees.
Chemical Information
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CAS No. 2140-72-9
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Appearance Solid
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Molecular Weight 257.25
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Formula C10H15N3O5
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Color White to off-white
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SMILES
O[C@H]1[C@@H](OC)[C@H](N2C(N=C(N)C=C2)=O)O[C@@H]1CO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
H2O : 58.33 mg/mL (226.74 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (388.73 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (277 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Steven S Carroll, et al. Inhibition of hepatitis C virus RNA replication by 2'-modified nucleoside analogs. J Biol Chem. 2003 Apr 4;278(14):11979-84 [Content Brief]
[3]. Del Vecchio AM, et al. Small molecule and biologic inhibitors of hepatitis C virus: a symbiotic approach. Mini Rev Med Chem. 2006 Nov;6(11):1263-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.8873 mL | 19.4363 mL | 38.8727 mL | 97.1817 mL |
| 5 mM | 0.7775 mL | 3.8873 mL | 7.7745 mL | 19.4363 mL | |
| 10 mM | 0.3887 mL | 1.9436 mL | 3.8873 mL | 9.7182 mL | |
| 15 mM | 0.2592 mL | 1.2958 mL | 2.5915 mL | 6.4788 mL | |
| 20 mM | 0.1944 mL | 0.9718 mL | 1.9436 mL | 4.8591 mL | |
| 25 mM | 0.1555 mL | 0.7775 mL | 1.5549 mL | 3.8873 mL | |
| 30 mM | 0.1296 mL | 0.6479 mL | 1.2958 mL | 3.2394 mL | |
| 40 mM | 0.0972 mL | 0.4859 mL | 0.9718 mL | 2.4295 mL | |
| 50 mM | 0.0777 mL | 0.3887 mL | 0.7775 mL | 1.9436 mL | |
| 60 mM | 0.0648 mL | 0.3239 mL | 0.6479 mL | 1.6197 mL | |
| 80 mM | 0.0486 mL | 0.2430 mL | 0.4859 mL | 1.2148 mL | |
| 100 mM | 0.0389 mL | 0.1944 mL | 0.3887 mL | 0.9718 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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