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4β-Hydroxywithanolide E

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4β-Hydroxywithanolide E, isolated from Physalis peruviana L., inhibits adipocyte differentiation of 3T3-L1 cells through modulation of mitotic clonal expansion. 4β-Hydroxywithanolide E is an adipogenesis inhibitor and inhibits PPARγ, C/EBPα, and the adipocyte-specific molecule aP2 mRNA expression.

For research use only. We do not sell to patients.

Purity: 98%
CAS No.: 54334-04-2
Formula: C₂₈H₃₈O₈
Molecular Weight:502.60

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

4β-Hydroxywithanolide E Chemical Structure

CAS No.: 54334-04-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

All PPAR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
ACHN	IC₅₀	>2 μM Compound: 12	Cytotoxicity against human ACHN cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human ACHN cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
ACHN	IC₅₀	563 nM Compound: 5	Sensitization of TRAIL-induced apoptosis in human ACHN cells assessed as reduction in cell viability preincubated for 2 hrs followed by addition of TRAIL measured after 18 hrs by MTS assay Sensitization of TRAIL-induced apoptosis in human ACHN cells assessed as reduction in cell viability preincubated for 2 hrs followed by addition of TRAIL measured after 18 hrs by MTS assay	[PMID: 28257574]
CWR22R	IC₅₀	0.09 μM Compound: 12	Cytotoxicity against human 22Rv1 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human 22Rv1 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
HEK293	IC₅₀	0.04 μM Compound: 4	Inhibition of TNFalpha-activated nuclear factor-kappa B activity (unknown origin) expressed in HEK293 cells incubated for 6 hrs by luciferase reporter gene based assay Inhibition of TNFalpha-activated nuclear factor-kappa B activity (unknown origin) expressed in HEK293 cells incubated for 6 hrs by luciferase reporter gene based assay	[PMID: 27210437]
HFF	IC₅₀	>2 μM Compound: 12	Cytotoxicity against HFF cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against HFF cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
LNCaP	IC₅₀	0.16 μM Compound: 12	Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
M14	IC₅₀	>2 μM Compound: 12	Cytotoxicity against human M14 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human M14 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
MCF7	GI₅₀	1 μM Compound: 2	Antiproliferative activity against human MCF7 cells expressing estrogen receptor after 48 hrs by SRB assay Antiproliferative activity against human MCF7 cells expressing estrogen receptor after 48 hrs by SRB assay	[PMID: 20438092]
MDA-MB-231	GI₅₀	2.1 μM Compound: 2	Antiproliferative activity against estrogen receptor deficient human MDA-MB-231 cells after 48 hrs by SRB assay Antiproliferative activity against estrogen receptor deficient human MDA-MB-231 cells after 48 hrs by SRB assay	[PMID: 20438092]
RAW264.7	IC₅₀	0.32 μM Compound: 4	Inhibition of lipopolysaccharide-induced nitric oxide production in mouse RAW264.7 cells pre-incubated for 30 mins before LPS stimulation for 20 hrs by Griess reagent based assay Inhibition of lipopolysaccharide-induced nitric oxide production in mouse RAW264.7 cells pre-incubated for 30 mins before LPS stimulation for 20 hrs by Griess reagent based assay	[PMID: 27210437]
RAW264.7	IC₅₀	1.8 μM Compound: 1	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 30 mins followed by LPS challenge and measured after 18 hrs by Griess assay Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 30 mins followed by LPS challenge and measured after 18 hrs by Griess assay	[PMID: 30649869]
SK-BR-3	GI₅₀	1.7 μM Compound: 2	Antiproliferative activity against estrogen receptor deficient human SKBR3 cells after 48 hrs by SRB assay Antiproliferative activity against estrogen receptor deficient human SKBR3 cells after 48 hrs by SRB assay	[PMID: 20438092]
SK-MEL-28	IC₅₀	>2 μM Compound: 12	Cytotoxicity against human SK-MEL-28 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human SK-MEL-28 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
T47D	GI₅₀	2.2 μM Compound: 2	Antiproliferative activity against human T47D cells expressing estrogen receptor after 48 hrs by SRB assay Antiproliferative activity against human T47D cells expressing estrogen receptor after 48 hrs by SRB assay	[PMID: 20438092]

Chemical Information

CAS No. 54334-04-2
Appearance Solid
Molecular Weight 502.60
Formula C₂₈H₃₈O₈
Color White to light yellow
SMILES

C[C@]1(C2=O)[C@@]3([C@H](C=C2)O)[C@](C[C@]([C@@]4(CC[C@](O)5[C@@](O)([C@@](OC6=O)([H])CC(C)=C6C)C)O)([H])[C@]1([H])CC[C@@]45C)([H])O3
Structure Classification
- Steroids
Initial Source
- Plants
- Solanaceae
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Momochika Kumagai, et al. 4β-Hydroxywithanolide E and withanolide E from Physalis peruviana L. inhibit adipocyte differentiation of 3T3-L1 cells through modulation of mitotic clonal expansion. J Nat Med. 2021 Jan;75(1):232-239. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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