5-LOX-IN-2
Based on 1 Customer Validation
5-LOX-IN-2, an inhibitor of 5-lipoxygenase (5-LOX) with an IC50 of 0.33 μM, inhibits 5-LOX in a dose-dependent manner . 5-LOX-IN-2, reduces the cell viability of renal cancer cells and induces apoptosis, can be used for cancer research.
For research use only. We do not sell to patients.
- Purity: 98.12%
- CAS No.: 179691-97-5
- Formula: C17H16O4
- Molecular Weight:284.31
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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5-LOX 0.33 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 786-0 | IC50 |
>100 μM
Compound: 10b
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Cytotoxicity against VHL-positive human 786-0 cells assessed as reduction in cell viability incubated for 4 days by XTT assay
Cytotoxicity against VHL-positive human 786-0 cells assessed as reduction in cell viability incubated for 4 days by XTT assay
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[PMID: 31260889] |
| 786-0 | IC50 |
34.8 μM
Compound: 10b
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Cytotoxicity against VHL-deficient human 786-0 cells assessed as reduction in cell viability incubated for 4 days by XTT assay
Cytotoxicity against VHL-deficient human 786-0 cells assessed as reduction in cell viability incubated for 4 days by XTT assay
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[PMID: 31260889] |
| RCC4 | IC50 |
8 μM
Compound: 10b
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Cytotoxicity against VHL-deficient human RCC4 cells assessed as reduction in cell viability incubated for 4 days by XTT assay
Cytotoxicity against VHL-deficient human RCC4 cells assessed as reduction in cell viability incubated for 4 days by XTT assay
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[PMID: 31260889] |
| RCC4/VHL | IC50 |
99.6 μM
Compound: 10b
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Cytotoxicity against VHL-positive human RCC4/VHL cells assessed as reduction in cell viability incubated for 4 days by XTT assay
Cytotoxicity against VHL-positive human RCC4/VHL cells assessed as reduction in cell viability incubated for 4 days by XTT assay
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[PMID: 31260889] |
5-LOX-IN-2 (Compound 10b) (0-100 μM; 4 days) reduces the cell viability of renal cancer cells[1].
5-LOX-IN-2 (Compound 10b) (0-10 μM; 24 hours) increases in LC3B and p62 expression, blocks of the autophagic flux in RCC4 cells, and induces apoptosis by activation of the caspase-3 pathway leading to cell death[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RCC4, RCC10, 786.0 cells
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Concentration:0-100 μM
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Incubation Time:4 days
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Result:Reduced the cell viability of renal cancer cells and was more selective toward RCC4 and 786.0 cells which are deficient for the Von Hippel-Lindau (VHL) tumor suppressor gene.
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Cell Line:RCC4, RCC10, 786.0 cells
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Concentration:0-10 μM
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Incubation Time:24 hours
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Result:Increased in LC3B and p62 expression, blocked of the autophagic flux in RCC4 cells.
Stimulated in a dose ependent manner the cleavage of pro-caspase-3 only in the RCC4 cells which lack the VHL tumor suppressor.
Chemical Information
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CAS No. 179691-97-5
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Appearance Solid
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Molecular Weight 284.31
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Formula C17H16O4
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Color Off-white to light yellow
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SMILES
O=C(OCCC1=CC=CC=C1)/C=C/C2=CC(O)=CC=C2O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (351.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5173 mL | 17.5864 mL | 35.1729 mL | 87.9322 mL |
| 5 mM | 0.7035 mL | 3.5173 mL | 7.0346 mL | 17.5864 mL | |
| 10 mM | 0.3517 mL | 1.7586 mL | 3.5173 mL | 8.7932 mL | |
| 15 mM | 0.2345 mL | 1.1724 mL | 2.3449 mL | 5.8621 mL | |
| 20 mM | 0.1759 mL | 0.8793 mL | 1.7586 mL | 4.3966 mL | |
| 25 mM | 0.1407 mL | 0.7035 mL | 1.4069 mL | 3.5173 mL | |
| 30 mM | 0.1172 mL | 0.5862 mL | 1.1724 mL | 2.9311 mL | |
| 40 mM | 0.0879 mL | 0.4397 mL | 0.8793 mL | 2.1983 mL | |
| 50 mM | 0.0703 mL | 0.3517 mL | 0.7035 mL | 1.7586 mL | |
| 60 mM | 0.0586 mL | 0.2931 mL | 0.5862 mL | 1.4655 mL | |
| 80 mM | 0.0440 mL | 0.2198 mL | 0.4397 mL | 1.0992 mL | |
| 100 mM | 0.0352 mL | 0.1759 mL | 0.3517 mL | 0.8793 mL |
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