2. Anti-infection
  3. Antibiotic Bacterial Parasite
  4. Acetylspiramycin

Acetylspiramycin  (Synonyms: Spiramycin B; Spiramycin II; Foromacidin B)

Cat. No.: HY-B1916
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Acetylspiramycin (Spiramycin B) is an effective oral macrolide antibiotic produced by Streptomyces, It can inhibit the splenic lymphocyte transformation induced by phytohemagglutinin (PHA), LPS (HY-D1056) and antigen, reduce the procoagulant activity of macrophages, have good antibacterial effect on gram-positive bacteria, and is also an effective antigenic insect agent, which can be used to fight parasitic infection.

For research use only. We do not sell to patients.

Acetylspiramycin Chemical Structure

Acetylspiramycin Chemical Structure

CAS No. : 24916-51-6

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Solid + Solvent
10 mM * 1 mL in DMSO
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Based on 2 publication(s) in Google Scholar

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Description

Acetylspiramycin (Spiramycin B) is an effective oral macrolide antibiotic produced by Streptomyces, It can inhibit the splenic lymphocyte transformation induced by phytohemagglutinin (PHA), LPS (HY-D1056) and antigen, reduce the procoagulant activity of macrophages, have good antibacterial effect on gram-positive bacteria, and is also an effective antigenic insect agent, which can be used to fight parasitic infection[1][2][3][4].

IC50 & Target

Macrolide

 

In Vitro

Acetylspiramycin (0-40 μg/mL, 0-72 h) can dose-dependently inhibit lymphocyte proliferation in spleen cell culture medium supplemented with plant hemagglutinin (PHA, 10 μg/mL), and the extent of its effect depends on the time of addition[3].
Acetylspiramycin (25-100 μg/ml, 18 h) can reduce the phagocytosis of mouse peritoneal macrophages and has reversible toxicity to macrophages in vitro[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Acetylspiramycin Related Antibodies

Cell Proliferation Assay[3]

Cell Line: Spleen cells were cultured with PHA (10 μg/mL)
Concentration: 0-40 μg/mL
Incubation Time: 0-72 h
Result: Inhibited 3HT incorporation in a dose-dependent manner.
Inhibited the proliferation response of B lymphocytes to LPS.
Inhibited antigen-induced proliferation of SRBC-immunized mouse spleen cells.
In Vivo

Acetylspiramycin (25-200 mg/kg/day; Oral gavage (p.o.); 7 days) can significantly enhance the lymphocyte proliferation response of CBA/H mice to PHA, and reduce the activity of lymphokine in a dose-dependent manner[3].
Acetylspiramycin (50-200 mg/kg/day; Oral gavage (p.o.); 14 days) in CBA/H mouse models can increase the efficiency of phagocytosis in mice[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: CBA/H mice
Dosage: 25-200 mg/kg/day
Administration: Oral gavage (p.o.), 7 days
Result: Decreased lymphokine activity in a dose-dependent manner
Animal Model: CBA/H mice[4]
Dosage: 50-200 mg/kg/day
Administration: Oral gavage (p.o.), 14 days
Result: Increased the efficiency of phagocytosis in mice.
Molecular Weight

885.09

Formula

C45H76N2O15
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@](O[C@H](C)[C@@H](O[C@@](O[C@@H](C)[C@@H]2O)([H])C[C@]2(O)C)[C@@H]1N(C)C)([H])O[C@@H]([C@H](C[C@H]([C@H](/C=C/C=C/C3)O[C@](CC[C@@H]4N(C)C)([H])O[C@@H]4C)C)CC=O)[C@H]([C@](CC(O[C@@H]3C)=O)([H])OC(C)=O)OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL (56.49 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1298 mL 5.6491 mL 11.2983 mL
5 mM 0.2260 mL 1.1298 mL 2.2597 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (2.82 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (2.82 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1298 mL 5.6491 mL 11.2983 mL 28.2457 mL
5 mM 0.2260 mL 1.1298 mL 2.2597 mL 5.6491 mL
10 mM 0.1130 mL 0.5649 mL 1.1298 mL 2.8246 mL
15 mM 0.0753 mL 0.3766 mL 0.7532 mL 1.8830 mL
20 mM 0.0565 mL 0.2825 mL 0.5649 mL 1.4123 mL
25 mM 0.0452 mL 0.2260 mL 0.4519 mL 1.1298 mL
30 mM 0.0377 mL 0.1883 mL 0.3766 mL 0.9415 mL
40 mM 0.0282 mL 0.1412 mL 0.2825 mL 0.7061 mL
50 mM 0.0226 mL 0.1130 mL 0.2260 mL 0.5649 mL
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Acetylspiramycin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Acetylspiramycin24916-51-6Spiramycin B Spiramycin II Foromacidin BAntibioticBacterialParasiteCryptosporidiosisantibioticinfectiontoxoplasmosisderivativegram-positiveparasiteInhibitorinhibitorinhibit

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