1. Apoptosis
  2. Bcl-2 Family
  3. Bz 423

Bz 423 (Synonyms: BZ48)

Cat. No.: HY-13108 Purity: 99.79%
Handling Instructions

Bz 423 is a pro-apoptotic 1,4-benzodiazepine with therapeutic properties in murine models of lupus demonstrating selectivity for autoreactive lymphocytes, and activates Bax and Bak.

For research use only. We do not sell to patients.

Bz 423 Chemical Structure

Bz 423 Chemical Structure

CAS No. : 216691-95-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 105 In-stock
Estimated Time of Arrival: December 31
5 mg USD 95 In-stock
Estimated Time of Arrival: December 31
10 mg USD 155 In-stock
Estimated Time of Arrival: December 31
50 mg USD 655 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Bz 423 is a pro-apoptotic 1,4-benzodiazepine with therapeutic properties in murine models of lupus demonstrating selectivity for autoreactive lymphocytes, and activates Bax and Bak.

IC50 & Target

Bax

 

Bak

 

In Vitro

Bz-423 induces a larger superoxide response in Ramos cells, and causes rapid death of Ramos B cells. Bz-423 activates Bax and Bak, but does not activate ASK1/JNK in Ramos cells. In Ramos cells Bz-423-induced superoxide leads to Bax and Bak activation via changes in Mcl-1 expression and functional activation of BH3-only proteins[1]. Bz-423 rapidly increases superoxide levels in MEFs within 1 h and the magnitude of the increase is concentration-dependent. Bz-423-induced apoptosis dependents upon superoxide[2].

Molecular Weight

440.92

Formula

C₂₇H₂₁ClN₂O₂

CAS No.

216691-95-1

SMILES

O=C1N(C)C2=CC=C(Cl)C=C2C(C3=CC=C(O)C=C3)=NC1CC4=CC=C5C=CC=CC5=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 125 mg/mL (283.50 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2680 mL 11.3399 mL 22.6798 mL
5 mM 0.4536 mL 2.2680 mL 4.5360 mL
10 mM 0.2268 mL 1.1340 mL 2.2680 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.72 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.72 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Kinase Assay
[1]

To detect ASK1 activity, inactive GST-MKK6 (0.1 mg) is incubated (15 min, 25°C) with immunoprecipitated ASK1 in kinase assay buffer. Subsequently, GST-p38α (0.5 µg) is added to this reaction and incubated (15 min, 25°C) followed by detection of phospho-GST-p38 via immunoblot.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

Bz 423BZ48Bz423Bz-423BZ 48BZ-48Bcl-2 FamilyInhibitorinhibitorinhibit

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Product Name:
Bz 423
Cat. No.:
HY-13108
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