2. Apoptosis
  3. Bcl-2 Family
  4. Bz 423

Bz 423 is a pro-apoptotic 1,4-benzodiazepine with therapeutic properties in murine models of lupus demonstrating selectivity for autoreactive lymphocytes, and activates Bax and Bak.

For research use only. We do not sell to patients.

Bz 423 Chemical Structure

Bz 423 Chemical Structure

CAS No. : 216691-95-1

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 105 In-stock
Solution
10 mM * 1 mL in DMSO USD 105 In-stock
Solid
5 mg USD 95 In-stock
10 mg USD 155 In-stock
50 mg USD 655 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Bz 423 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Bz 423

View All Bcl-2 Family Isoform Specific Products:

View All Isoforms
Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Bz 423 is a pro-apoptotic 1,4-benzodiazepine with therapeutic properties in murine models of lupus demonstrating selectivity for autoreactive lymphocytes, and activates Bax and Bak.

IC50 & Target[1]

Bax

 

Bak

 

In Vitro

Bz-423 induces a larger superoxide response in Ramos cells, and causes rapid death of Ramos B cells. Bz-423 activates Bax and Bak, but does not activate ASK1/JNK in Ramos cells. In Ramos cells Bz-423-induced superoxide leads to Bax and Bak activation via changes in Mcl-1 expression and functional activation of BH3-only proteins[1]. Bz-423 rapidly increases superoxide levels in MEFs within 1 h and the magnitude of the increase is concentration-dependent. Bz-423-induced apoptosis dependents upon superoxide[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bz 423 Related Antibodies
Molecular Weight

440.92

Formula

C27H21ClN2O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1N(C)C2=CC=C(Cl)C=C2C(C3=CC=C(O)C=C3)=NC1CC4=CC=C5C=CC=CC5=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 125 mg/mL (283.50 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2680 mL 11.3399 mL 22.6798 mL
5 mM 0.4536 mL 2.2680 mL 4.5360 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.72 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (4.72 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Kinase Assay
[1]

To detect ASK1 activity, inactive GST-MKK6 (0.1 mg) is incubated (15 min, 25°C) with immunoprecipitated ASK1 in kinase assay buffer. Subsequently, GST-p38α (0.5 µg) is added to this reaction and incubated (15 min, 25°C) followed by detection of phospho-GST-p38 via immunoblot.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2680 mL 11.3399 mL 22.6799 mL 56.6996 mL
5 mM 0.4536 mL 2.2680 mL 4.5360 mL 11.3399 mL
10 mM 0.2268 mL 1.1340 mL 2.2680 mL 5.6700 mL
15 mM 0.1512 mL 0.7560 mL 1.5120 mL 3.7800 mL
20 mM 0.1134 mL 0.5670 mL 1.1340 mL 2.8350 mL
25 mM 0.0907 mL 0.4536 mL 0.9072 mL 2.2680 mL
30 mM 0.0756 mL 0.3780 mL 0.7560 mL 1.8900 mL
40 mM 0.0567 mL 0.2835 mL 0.5670 mL 1.4175 mL
50 mM 0.0454 mL 0.2268 mL 0.4536 mL 1.1340 mL
60 mM 0.0378 mL 0.1890 mL 0.3780 mL 0.9450 mL
80 mM 0.0283 mL 0.1417 mL 0.2835 mL 0.7087 mL
100 mM 0.0227 mL 0.1134 mL 0.2268 mL 0.5670 mL
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Bz 423 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bz 423216691-95-1BZ48Bz423Bz-423BZ 48BZ-48Bcl-2 FamilyInhibitorinhibitorinhibit

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