Cat. No.: HY-114429

CB-6644

17 Cited Publications

Customer Review

Based on 17 publication(s) in Google Scholar

CB-6644 is a selective inhibitor of RUVBL1/2 complex with anti-cancer activity. CB-6644 blocks the ATPase activity of RUVBL1/2 with an IC₅₀ of 15 nM.

For research use only. We do not sell to patients.

Purity: 98.76%
CAS No.: 2316817-88-4
Formula: C₂₉H₃₄ClFN₄O₅
Molecular Weight:573.06

Storage:

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

CB-6644 Chemical Structure

CAS No.: 2316817-88-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (17)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

CB-6644 is a selective inhibitor of RUVBL1/2 complex with anti-cancer activity. CB-6644 blocks the ATPase activity of RUVBL1/2 with an IC₅₀ of 15 nM^[1].

All DNA/RNA Synthesis Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.3 μM Compound: 9; CB-6644	Anti-proliferative activity against human A549 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay Anti-proliferative activity against human A549 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay	[PMID: 35364523]
HCT-116	IC₅₀	0.08 μM Compound: 9; CB-6644	Anti-proliferative activity against human HCT-116 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay Anti-proliferative activity against human HCT-116 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay	[PMID: 35364523]
MDA-MB-231	IC₅₀	0.7 μM Compound: 9; CB-6644	Anti-proliferative activity against human MDA-MB-231 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay Anti-proliferative activity against human MDA-MB-231 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay	[PMID: 35364523]

In Vitro

CB-6644 (20 μM) interacts with the RUVBL1/2 complex in Ramos cells^[1].
CB-6644 (0.001-10 μM; 72 hours) potently kills 123 cell lines (including HCT116, NCI-1975, and HT29 cells) with an EC₅₀ range of 41 to 785 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

123 cell lines such as HCT116, NCI-1975, and HT29 cells
Concentration:

0.001, 0.01, 0.1, 1, and 10 μM
Incubation Time:

72 hours
Result:

Potently killed cells with an EC₅₀ range of 41 to 785 nM.

In Vivo

CB-6644 (150 mg/kg, oral administration, 10 days of treatment for SCID-beige mice bearing Ramos xenograft models, 30 days of treatment for SCID-beige mice bearing RPMI8226 xenograft models) has antitumor activity in xenograft tumor models with tumor growth inhibitions (TGIs) of 68 and 81%, respectively in Ramos xenograft models and RPMI8226 xenograft models^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

SCID-beige mice bearing human tumor xenografts derived from either Burkitt’s lymphoma (Ramos) or multiple myeloma (RPMI8226) cell lines^[1]
Dosage:

150 mg/kg
Administration:

Oral gavage once (qd) or twice (BID) daily, 10 days of treatment for Ramos, 30 days of treatment for RPMI8226
Result:

There was no significant bodyweight loss in mice. TGI of 68 and 81% in Ramos and RPMI8226, respectively.

Chemical Information

CAS No. 2316817-88-4
Appearance Solid
Molecular Weight 573.06
Formula C₂₉H₃₄ClFN₄O₅
Color White to light yellow
SMILES

COCC1=C(NC(CC(C)(C)CNC(C2=C(OCC)C=C(F)C(Cl)=C2)=O)=O)C(N(N1CCC3)C4=C3C=CC=C4)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Publications (17)

All Bio/Physico-chemical AssayIn Vivo Efficacy Study

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Journal Impact Factor

Nature

The P-loop NTPase RUVBL2 is a conserved clock component across eukaryotes. [Abstract]

2025 Jun;642(8066):165-173. PMID: 40140583

CB-6644 purchased from MedChemExpress. Usage Cited in: Nature. 2025 Jun;642(8066):165-173. [Abstract]

The inhibitory effects of CB-6644 on the ATPase activity of human RUVBL1/2.

CB-6644 purchased from MedChemExpress. Usage Cited in: Nature. 2025 Jun;642(8066):165-173. [Abstract]

Normalized luminescence rhythm of Bmal1-dLuc U2OS cells shows the period lengthening for 2 h in the presence of CB-6644 (3 μM), n = 3 biologically independent cells.

CB-6644 purchased from MedChemExpress. Usage Cited in: Nature. 2025 Jun;642(8066):165-173. [Abstract]

Left panel: statistical analysis of the period length changes of Drosophila melanogaster. Right panel: represents of double-plotted averaged actogram of Drosophila melanogaster with CB-6644 or control treatment.

CB-6644 purchased from MedChemExpress. Usage Cited in: Nature. 2025 Jun;642(8066):165-173. [Abstract]

Left panel: statistical analysis of the period length of Neurospora crassa with CB-6644 treatment. Right panel: normalized luminescence rhythm of Neurospora crassa treated by CB-6644.

CB-6644 purchased from MedChemExpress. Usage Cited in: Nature. 2025 Jun;642(8066):165-173. [Abstract]

Left panel: statistical analysis of the period length of Arabidopsis thaliana with CB-6644 treatment. Right panel: normalized luminescence rhythm of Arabidopsis thaliana treated by CB-6644, cordycepin, and adenosine.
Gut

Targeting MYC effector functions in pancreatic cancer by inhibiting the ATPase RUVBL1/2. [Abstract]

2024 May 31:gutjnl-2023-331519. PMID: 38821858
Cell Host Microbe

Mycobacterium tuberculosis suppresses host DNA repair to boost its intracellular survival. [Abstract]

2023 Nov 8;31(11):1820-1836.e10. PMID: 37848028
Cell Death Dis

A common druggable signature of oncogenic c-Myc, mutant KRAS and mutant p53 reveals functional redundancy and competition among oncogenes in cancer. [Abstract]

2024 Aug 31;15(8):638. PMID: 39217152
Cell Rep Phys Sci

2024 May 13.
Oncogene

RUVBL1 promotes enzalutamide resistance of prostate tumors through the PLXNA1-CRAF-MAPK pathway. [Abstract]

2022 Jun;41(23):3239-3250. PMID: 35508542
Cell Rep

Regionally defined proteomic profiles of human cerebral tissue and organoids reveal conserved molecular modules of neurodevelopment. [Abstract]

2022 May 24;39(8):110846. PMID: 35613588
Front Immunol

RUVBL1/2 Complex Regulates Pro-Inflammatory Responses in Macrophages via Regulating Histone H3K4 Trimethylation. [Abstract]

2021 Jun 4:12:679184. PMID: 34276666
Int J Mol Sci

Molecular Signatures of CB-6644 Inhibition of the RUVBL1/2 Complex in Multiple Myeloma. [Abstract]

2024 Aug 20;25(16):9022. PMID: 39201707
Mol Oncol

Patient therapy outcome modeling in cancer organoids is improved by cancer-associated fibroblasts and organoid assembly convolution. [Abstract]

2026 Jun 5. PMID: 42246237
iScience

2026 Mar 4;29(4).
bioRxiv

2026 May 14.
bioRxiv

2025 Dec 18.
bioRxiv

2025 Dec 23.
bioRxiv

2023 Dec 21.
Research Square Preprint

2023 Nov 20.
bioRxiv

July 11, 2021.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (174.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7450 mL

8.7251 mL

17.4502 mL

5 mM

0.3490 mL

1.7450 mL

3.4900 mL

10 mM

0.1745 mL

0.8725 mL

1.7450 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (3.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (3.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (3.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Assimon VA, et al. CB-6644 Is a Selective Inhibitor of the RUVBL1/2 Complex with Anticancer Activity. ACS Chem Biol. 2019 Feb 15;14(2):236-244. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7450 mL	8.7251 mL	17.4502 mL	43.6254 mL
	5 mM	0.3490 mL	1.7450 mL	3.4900 mL	8.7251 mL
	10 mM	0.1745 mL	0.8725 mL	1.7450 mL	4.3625 mL
	15 mM	0.1163 mL	0.5817 mL	1.1633 mL	2.9084 mL
	20 mM	0.0873 mL	0.4363 mL	0.8725 mL	2.1813 mL
	25 mM	0.0698 mL	0.3490 mL	0.6980 mL	1.7450 mL
	30 mM	0.0582 mL	0.2908 mL	0.5817 mL	1.4542 mL
	40 mM	0.0436 mL	0.2181 mL	0.4363 mL	1.0906 mL
	50 mM	0.0349 mL	0.1745 mL	0.3490 mL	0.8725 mL
	60 mM	0.0291 mL	0.1454 mL	0.2908 mL	0.7271 mL
	80 mM	0.0218 mL	0.1091 mL	0.2181 mL	0.5453 mL
	100 mM	0.0175 mL	0.0873 mL	0.1745 mL	0.4363 mL

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CB-6644

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) CB-6644

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

Publications (17)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

CB-6644

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) CB-6644

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

Publications (17)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

CB-6644 Related Classifications

HY-114429 Related Classifications