Indole-3-carbinol
Based on 19 publication(s) in Google Scholar
Indole-3-carbinol (I3C) inhibits NF-κB activity and also is an Aryl hydrocarbon receptor (AhR) agonist, and an inhibitor of WWP1 (WW domain-containing ubiquitin E3 ligase 1).
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- Pureté : 99.16%
- CAS No.: 700-06-1
- Formule: C9H9NO
- Masse moléculaire:147.18
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Indole-3-carbinol
More- Cancer Res. 2026 Jun 15.
- Cell Death Dis. 2026 May 19;17(1):635. [Abstract]
- Mol Biol Lett. 2025 May 3;30(1):56. [Abstract]
- Phytomedicine. 2026 May:154:158040. [Abstract]
- Phytomedicine. 2026 Jan:150:157740. [Abstract]
- Phytomedicine. 2025 Jun:141:156692. [Abstract]
- NPJ Precis Oncol. 2024 Jul 16;8(1):144. [Abstract]
- Int J Biol Macromol. 2026 Jun:369:152765. [Abstract]
- Brain Behav Immun. 2020 Nov:90:108-137. [Abstract]
- Int Immunopharmacol. 2026 Jan 15:169:116009. [Abstract]
- Front Pharmacol. 2022 Jun 6:13:924174. [Abstract]
- Molecules. 2018 Oct 11;23(10):2600. [Abstract]
- Mol Nutr Food Res. 2025 Sep 28:e70265. [Abstract]
- Fish Shellfish Immunol. 2023 Oct:141:109037. [Abstract]
- Discov Oncol. 2025 Feb 22;16(1):224. [Abstract]
- Dev Comp Immunol. 2021 Oct:123:104148. [Abstract]
- Res Sq. 2024 Jul 19.
- Egyptian Journal of Basic and Applied Sciences. 2024 May 31.
- Research Square Print. 2023 Mar 27.
Customer Validation & Images
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IHC
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WB
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WB
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IHC
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RT-PCR
Voir tous les produits spécifiques à Isoform Endogenous Metabolite
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Activité biologique
Description
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NF-κB |
AhR |
Human Endogenous Metabolite |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| GL261 | IC50 |
100 μM
Compound: indole-3-carbinol
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Anti-proliferative activity against mouse GL261 cells assessed as cell viability incubated for 2 hrs by CCK8 assay
Anti-proliferative activity against mouse GL261 cells assessed as cell viability incubated for 2 hrs by CCK8 assay
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[PMID: 37861443] |
| U-251 | EC50 |
390 μM
Compound: 3; I3C
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Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay
Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay
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[PMID: 28256372] |
| U-87MG ATCC | EC50 |
290 μM
Compound: 3; I3C
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Cytotoxicity against human U87 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay
Cytotoxicity against human U87 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay
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[PMID: 28256372] |
| U-87MG ATCC | IC50 |
>100 μM
Compound: indole-3-carbinol
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Anti-proliferative activity against human U-87 MG cells assessed as cell viability incubated for 2 hrs by CCK8 assay
Anti-proliferative activity against human U-87 MG cells assessed as cell viability incubated for 2 hrs by CCK8 assay
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[PMID: 37861443] |
| U-87MG ATCC | IC50 |
526 μM
Compound: 1, I3C
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Antiproliferative activity against human U87MG cells assessed as inhibition of cell growth after 48 hrs by MTS assay
Antiproliferative activity against human U87MG cells assessed as inhibition of cell growth after 48 hrs by MTS assay
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[PMID: 23419325] |
In Vitro
It is found that Indole-3-carbinol (I3C) inhibits the proliferation of THP-1 cells in a dose- and time dependent manner with minimal toxicity over normal monocytes. The AhR target genes (CYP1A1, IL1β) are overexpressed upon Indole-3-carbinol treatment (p<0.05 to p<0.001). The antiproliferative effects of Indole-3-carbinol are in association with programing cell death. Indole-3-carbinol downregulates BCL2 and upregulates FasR in THP-1 cells (p<0.05 to p<0.001). G1 cell cycle arrest is also observed using flow cytometry. G1-acting cell cycle genes (P21, P27 and P53) are overexpressed (p<0.05 to p<0.001), while CDK2 is downregulated upon Indole-3-carbinol treatment (p<0.01 to p<0.001)[1].Indole-3-carbinol suppresses NF-κB activity and stimulates the p53 pathway in pre-B acute lymphoblastic leukemia cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Essai clinique
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 700-06-1
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Appearance Solid
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Masse moléculaire 147.18
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Formule C9H9NO
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Color White to off-white
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SMILES
OCC1=CNC2=C1C=CC=C2
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Synonyms
Indole-3-methanol
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (19)
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Journal Impact Factor
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Most Recent
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Cell Death Dis
E2F-mediated activation of mTORC1 through the ubiquitin-proteasome system promotes lung adenocarcinoma progression. [Abstract]2026 May 19;17(1):635. PMID: 42156726 -
Mol Biol Lett
YY1 induced USP13 transcriptional activation drives the malignant progression of hepatocellular carcinoma by deubiquitinating WWP1. [Abstract]2025 May 3;30(1):56. PMID: 40319251
Indole-3-carbinol purchased from MedChemExpress. Usage Cited in: Mol Biol Lett. 2025 May 3;30(1):56. [Abstract]
Indole-3-carbinol (I3C: 20/40 mg/kg). Immunohistochemical analysis of Ki67 expression in tumors.
Indole-3-carbinol purchased from MedChemExpress. Usage Cited in: Mol Biol Lett. 2025 May 3;30(1):56. [Abstract]
Indole-3-carbinol (I3C: 20/40 mg/kg). Protein levels of USP13 and WWP1 in nude mouse tumors were detected by Western blot.
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Phytomedicine
Indole-3-carbinol attenuates cisplatin-induced premature ovarian failure by activating Nrf2 through competitive binding of Keap1. [Abstract]2026 May:154:158040. PMID: 41818942 -
Phytomedicine
Bruceantin inhibits the c-Myc/RL27A axis to suppress tumor progression in hepatocellular carcinoma. [Abstract]2026 Jan:150:157740. PMID: 41477978 -
Phytomedicine
Indole-3-carbinol inhibits PD-L1-mediated immune evasion in hepatocellular carcinoma via suppressing NF-κB p105 Ubiquitination. [Abstract]2025 Jun:141:156692. PMID: 40215823 -
NPJ Precis Oncol
2024 Jul 16;8(1):144. PMID: 39014007
Indole-3-carbinol purchased from MedChemExpress. Usage Cited in: NPJ Precis Oncol. 2024 Jul 16;8(1):144. [Abstract]
The combined use of indole-3-carbinol (I3C) and SHP099 significantly inhibited pAKT levels. Different CRC cell lines were treated with I3C (200 μM) or SHP099 (20 μM), respectively.
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Int J Biol Macromol
Aryl hydrocarbon receptor alleviates intestinal ischemia/reperfusion-induced epithelial barrier damage by inhibiting the 5-hydroxytryptamine-mediated complement C3 cascade. [Abstract]2026 Jun:369:152765. PMID: 42214532 -
Brain Behav Immun
Protective effect of Indole-3-carbinol, an NF-κB inhibitor in experimental paradigm of Parkinson's disease: In silico and in vivo studies. [Abstract]2020 Nov:90:108-137. PMID: 32800927 -
Int Immunopharmacol
I3C protects IPEC-J2 cells by inhibiting ferroptosis through activation of the AhR-Nrf2-SLC7A11-GPX4 pathways. [Abstract]2026 Jan 15:169:116009. PMID: 41386181 -
Front Pharmacol
Indole-3-Carbinol (I3C) Protects the Heart From Ischemia/Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Cellular Apoptosis in Mice. [Abstract]2022 Jun 6:13:924174. PMID: 35734410 -
Molecules
2018 Oct 11;23(10):2600. PMID: 30314280
Indole-3-carbinol purchased from MedChemExpress. Usage Cited in: Molecules. 2018 Oct 11;23(10):2600. [Abstract]
The cells are cultured with Indole-3-carbinol (I3C: 50 μM) or 3MC (1 μM) in the presence of IPRN (20 μM). The IPRN-induced inhibition of nucleocytoplasmic shuttling of AhR is suppressed by I3C and 3MC.
Indole-3-carbinol purchased from MedChemExpress. Usage Cited in: Molecules. 2018 Oct 11;23(10):2600. [Abstract]
The cells are cultured with Indole-3-carbinol (I3C: 50 μM) or 3MC (1 μM) in the presence of IPRN (20 μM). The mRNA expression of CYP1A1 is increased after the cotreatment of I3C and 3MC.
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Mol Nutr Food Res
The Combination of Indole-3-Carbinol and Tryptophan Provides Additive Benefits in Enhancing Innate Immunity Against Salmonella Colitis in Mice. [Abstract]2025 Sep 28:e70265. PMID: 41017216 -
Fish Shellfish Immunol
NEDD4 activates mitophagy by interacting with LC3 to restrain reactive oxygen species and apoptosis in Apostichopus japonicus challenged with Vibrio splendidus. [Abstract]2023 Oct:141:109037. PMID: 37640120 -
Discov Oncol
Indole-3-carbinol prevented tumor progression and potentiated PD1ab therapy by upregulating PTEN in colorectal cancer. [Abstract]2025 Feb 22;16(1):224. PMID: 39985695 -
Dev Comp Immunol
An HECT domain ubiquitin ligase CgWWP1 regulates granulocytes proliferation in oyster Crassostrea gigas. [Abstract]2021 Oct:123:104148. PMID: 34097916 -
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Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (679.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.99 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.99 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
Pureté et documentation
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Fiche technique (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Mohammadi S, et al. Indole-3-carbinol induces G1 cell cycle arrest and apoptosis through aryl hydrocarbon receptor in THP-1 monocytic cell line.J Recept Signal Transduct Res. 2017 Oct;37(5):506-514. [Content Brief]
[2]. Safa M, et al. Indole-3-carbinol suppresses NF-κB activity and stimulates the p53 pathway in pre-B acute lymphoblastic leukemia cells. Tumour Biol. 2015 May;36(5):3919-30. [Content Brief]
[3]. Lee YR, et al. Reactivation of PTEN tumor suppressor for cancer treatment through inhibition of a MYC-WWP1 inhibitory pathway. Science. 2019 May 17;364(6441). pii: eaau0159. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.7944 mL | 33.9720 mL | 67.9440 mL | 169.8600 mL |
| 5 mM | 1.3589 mL | 6.7944 mL | 13.5888 mL | 33.9720 mL | |
| 10 mM | 0.6794 mL | 3.3972 mL | 6.7944 mL | 16.9860 mL | |
| 15 mM | 0.4530 mL | 2.2648 mL | 4.5296 mL | 11.3240 mL | |
| 20 mM | 0.3397 mL | 1.6986 mL | 3.3972 mL | 8.4930 mL | |
| 25 mM | 0.2718 mL | 1.3589 mL | 2.7178 mL | 6.7944 mL | |
| 30 mM | 0.2265 mL | 1.1324 mL | 2.2648 mL | 5.6620 mL | |
| 40 mM | 0.1699 mL | 0.8493 mL | 1.6986 mL | 4.2465 mL | |
| 50 mM | 0.1359 mL | 0.6794 mL | 1.3589 mL | 3.3972 mL | |
| 60 mM | 0.1132 mL | 0.5662 mL | 1.1324 mL | 2.8310 mL | |
| 80 mM | 0.0849 mL | 0.4247 mL | 0.8493 mL | 2.1233 mL | |
| 100 mM | 0.0679 mL | 0.3397 mL | 0.6794 mL | 1.6986 mL |
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