1. Stem Cell/Wnt
    TGF-beta/Smad
    Anti-infection
  2. TGF-beta/Smad
    Influenza Virus
  3. Oxymatrine

Oxymatrine 

Cat. No.: HY-N0158 Purity: >98.0%
Handling Instructions

Oxymatrine, an alkaloid from the roots of Sophora species, with anti-inflammatory, antifibrosis, and antitumor effects, inhibits the iNOS expression and TGF-β/Smad pathway. Oxymatrine inhibits bocavirus minute virus of canines (MVC) replication, reduces viral gene expression and decreases apoptosis induced by viral infection.

For research use only. We do not sell to patients.

Oxymatrine Chemical Structure

Oxymatrine Chemical Structure

CAS No. : 16837-52-8

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10 mM * 1  mL in DMSO USD 79 In-stock
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1 g USD 216 In-stock
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Customer Review

Based on 6 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Oxymatrine purchased from MCE. Usage Cited in: Front Bioeng Biotechnol. 2020 May 8;8:392.

    Acta2 expression after Signaling inhibitors by q-PCR. For inhibitor treatment, 24 h after seeding, the medium is removed and replaced with the TGF-β/Smad pathway inhibitor Oxymatrine (5 mg/mL) to block the TGF-β/Smad signaling pathways.

    Oxymatrine purchased from MCE. Usage Cited in: Exp Cell Res. 2020 Jun 11;112138.

    Protein expression of TGF-β1 and pSmad2 in renal tissues detected by immunoblotting. The TGF-β/Smad/TLR3 pathway is significantly inhibited after addition of Oxymatrine.
    • Biological Activity

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    Description

    Oxymatrine, an alkaloid from the roots of Sophora species, with anti-inflammatory, antifibrosis, and antitumor effects, inhibits the iNOS expression and TGF-β/Smad pathway. Oxymatrine inhibits bocavirus minute virus of canines (MVC) replication, reduces viral gene expression and decreases apoptosis induced by viral infection.

    In Vitro

    Oxymatrine, an alkaloid component extracted from the roots of Sophora species, has been shown to have antiinflammatory, antifibrosis, and antitumor effects and the ability to protect against myocardial damage, etc. The potential signaling pathways involved in the clinical application of oxymatrine might include the TGF-β/Smad, tolllike receptor 4/nuclear factor kappa-light-chain-enhancer of activated B cells, toll-like receptor9/TRAF6, Janus kinase/signal transduction and activator of transcription, phosphatidylinositol-3 kinase/Akt, delta-opioid receptorarrestinl-Bcl-2, CD40, epidermal growth factor receptor, nuclear factor erythroid-2-related factor 2/hemeoxygenase-1 signaling pathways, and dimethylarginine dimethylaminohydrolase/asymmetric dimethylarginine metabolism pathway[1]. Oxymatrine significantly inhibits the proliferation of DU145 and PC-3 cell lines in a time- and dose-dependent manner. By contrast, following treatment with oxymatrine, PNT1B healthy human prostate cell proliferation is not inhibited[2].

    In Vivo

    The volume and weight of tumors in mice significantly decreased in a dose-dependent manner. Oxymatrine may reduce prostate cancer cell growth by promoting cell apoptosis in vivo[2]. Oxymatrine is effective in reducing the production and deposition of collagen in the liver tissue of experimental rats. Oxymatrine could promote the expression of Smad 7 and inhibit the expression of Smad 3 and CBP in CCl4-induced hepatic fibrosis in SD rats, could modulate the fibrogenic signal transduction of TGFβ-Smad pathway[3].

    Molecular Weight

    264.36

    Formula

    C₁₅H₂₄N₂O₂

    CAS No.

    16837-52-8

    SMILES

    O=C1CCC[[email protected]]2([H])[[email protected]@]3([H])CCC[[email protected]@+]4([O-])[[email protected]@]3([H])[[email protected]](CCC4)([H])CN21

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (378.27 mM; Need ultrasonic)

    DMSO : 100 mg/mL (378.27 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7827 mL 18.9136 mL 37.8272 mL
    5 mM 0.7565 mL 3.7827 mL 7.5654 mL
    10 mM 0.3783 mL 1.8914 mL 3.7827 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (9.46 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (9.46 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: 2.5 mg/mL (9.46 mM); Clear solution; Need warming

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [2]

    DU145, PC-3 and PNT1B cell lines (3×104 cells/well) are seeded into 96-well plates and incubated overnight at 37°C in 5% CO2. Subsequently, the cells are incubated with different concentrations of oxymatrine (0, 2, 4, 6 and 8 mg/mL). MTT (10 mL; 5 mg/mL) is added and the mixture is incubated in darkness at 37°C for 2 h. Absorbance is measured at a wavelength of 490 nm using a microplate reader[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2][3]

    Rats: One hundred healthy male SD rats (weight 140-160 g) are used in the study. All 100 rats are randomLy divided into three groups: Control (n=20), Treatment (n=40) and Model group (n=40). For the model group, 300 g/L CCl4 soluted in liquid paraffin is injected subcutaneously at a dosage of 3 mL/kg twice per week[6]. The treated rats receive Oxymatrine celiac injections at 10 mg/kg twice a week besides the injection of CCl4[3].

    Mice: BALB/c homozygous (nu/nu) nude mice are used in the study. 24 tumor-bearing mice are randomLy divided into three groups: The control group is treated with PBS, and two groups are treated with different concentrations of oxymatrine (50 mg/kg and 100 mg/kg body weight). Oxymatrine is administered to the mice, using daily intraperitoneal injections[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Keywords:

    OxymatrineTGF-beta/SmadInfluenza VirusTransforming growth factor betaInhibitorinhibitorinhibit

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    Product name:
    Oxymatrine
    Cat. No.:
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