Cat. No.: HY-N0069

Solamargine

Name: Solamargine
Brand: MedChemExpress
SKU: HY-N0069
Price: 68.00 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Solamargin; δ-Solanigrine)

10 Cited Publications

Customer Review

Based on 10 publication(s) in Google Scholar

Solamargine, a derivative from the steroidal solasodine in Solanum species, exhibits anticancer activities in numerous types of cancer. Solamargine induces non-selective cytotoxicity and P-glycoprotein inhibition. Solamargine significantly inhibits migration and invasion of HepG2 cells by down-regulating MMP-2 and MMP-9 expression and activity.

For research use only. We do not sell to patients.

Purity: 99.87%
CAS No.: 20311-51-7
Formula: C₄₅H₇₃NO₁₅
Molecular Weight:868.06

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Solamargine Chemical Structure

CAS No.: 20311-51-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (10)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	8 μM Compound: 1, Solamargine	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 21482107]
A549	IC₅₀	8.18 μM Compound: SM	Cytotoxicity against human A549 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 24534485]
H9c2	IC₅₀	>20 μM Compound: 1, Solamargine	Cytotoxicity against rat H9c2 cells by MTT assay Cytotoxicity against rat H9c2 cells by MTT assay	[PMID: 21482107]
HCT-116	IC₅₀	3.8 μM Compound: 1, Solamargine	Cytotoxicity against human HCT116 cells by MTT assay Cytotoxicity against human HCT116 cells by MTT assay	[PMID: 21482107]
HeLa	IC₅₀	6 μM Compound: 1, Solamargine	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 21482107]
HepG2	IC₅₀	2.5 μM Compound: 1, Solamargine	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 21482107]
HepG2	IC₅₀	2.67 μM Compound: SM	Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 24534485]
K562	IC₅₀	5.2 μM Compound: 1, Solamargine	Cytotoxicity against human K562 cells by MTT assay Cytotoxicity against human K562 cells by MTT assay	[PMID: 21482107]
L02	IC₅₀	13.23 μM Compound: SM	Cytotoxicity against human HL-7702 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HL-7702 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 24534485]
L02	IC₅₀	13.5 μM Compound: 1, Solamargine	Cytotoxicity against human HL-7702 by MTT assay Cytotoxicity against human HL-7702 by MTT assay	[PMID: 21482107]
MCF7	IC₅₀	2.1 μM Compound: 1, Solamargine	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 21482107]
MCF7	IC₅₀	8.12 μM Compound: SM	Cytotoxicity against human MCF7 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 24534485]
PLC-PRF-5	ED₅₀	1.53 μg/mL Compound: solamargine	Cytotoxicity against human PLC/PRF/5 cells by microassay Cytotoxicity against human PLC/PRF/5 cells by microassay	[PMID: 2380724]
U-87MG ATCC	IC₅₀	3.2 μM Compound: 1, Solamargine	Cytotoxicity against human U87 cells by MTT assay Cytotoxicity against human U87 cells by MTT assay	[PMID: 21482107]

In Vitro

Solamargine (15 μg/ml; 72 hours) predominantly stimulates the cells of the G2/M phase to apoptosis^[3].
Solamargine (72 hours) dramatically inhibits the proliferation of SMMC-7721 and HepG2 cells in a dose- and time-dependent manner. The IC₅₀s are 9.21 and 19.88 μg/ml, respectively^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

SMMC-7721cells
Concentration:

15 μg/ml
Incubation Time:

72 hours
Result:

Displayed a significant increase of sub-G1.

In Vivo

Solamargine (10 mg/kg; intragastric administration; once daily for 8 days) exhibits an antitumor effect and promotes the apoptosis of GC in vivo^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female specific pathogen-free BALB/c nude mice weighing 18-20 g (6-8-weeks-old)^[4]
Dosage:

10 mg/kg
Administration:

Intragastric administration; once daily for 8 days
Result:

Tumor growth was significantly inhibited .

Chemical Information

CAS No. 20311-51-7
Appearance Solid
Molecular Weight 868.06
Formula C₄₅H₇₃NO₁₅
Color White to off-white
SMILES

C[C@H](CC1)CN[C@]21O[C@@]3([H])C[C@@]4([H])[C@]5([H])CC=C6C[C@@H](O[C@@]7([H])[C@H](O[C@@]8([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O8)[C@@H](O)[C@H](O[C@]9([H])O[C@@H](C)[C@H](O)[C@@H](O)[C@H]9O)[C@@H](CO)O7)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@@]3([H])[C@@H]2C
Synonyms

Solamargin; δ-Solanigrine
Structure Classification
- Alkaloids
- Piperidine Alkaloids
- Saccharides
- Steroids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (10)

All Cell Imaging/StainingCell Proliferation/Viability AssayFlow CytometryWBIn Vivo Efficacy StudyIHC

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Journal Impact Factor

Food Chem

Simultaneous quantification of polyphenols, glycoalkaloids and saponins in African nightshade leaves using ultra-high performance liquid chromatography tandem mass spectrometry with acid assisted hydrolysis and multivariate analysis. [Abstract]

2020 May 15:312:126030. PMID: 31911353
Int J Oncol

Solamargine inhibits gastric cancer progression by regulating the expression of lncNEAT1_2 via the MAPK signaling pathway. [Abstract]

2019 May;54(5):1545-1554. PMID: 30864686

Solamargine purchased from MedChemExpress. Usage Cited in: Int J Oncol. 2019 May;54(5):1545-1554. [Abstract]

Cellular morphology of AGS, BGC823, SGC7901, HGC27, and MGC803 gastric cancer cells after treatment with Solamargine (10 μM; 48 h). Solamargine treatment reduced cell volume, disrupted visible intercellular junctions, concentrated the nuclei, weakened cell attachment, and produced abundant cell debris.

Solamargine purchased from MedChemExpress. Usage Cited in: Int J Oncol. 2019 May;54(5):1545-1554. [Abstract]

Cell-cycle analysis of SGC7901, HGC27, and BGC823 gastric cancer cells after treatment with Solamargine (10 μM; 16 h), using PI staining and flow cytometry. Solamargine significantly increased the proportion of cells in the G2/M phase in all three cell lines.

Solamargine purchased from MedChemExpress. Usage Cited in: Int J Oncol. 2019 May;54(5):1545-1554. [Abstract]

Erk1/2 MAPK and p-Erk1/2 MAPK expression in SGC7901, BGC823, and HGC27 gastric cancer cells after treatment with Solamargine (10 μM; 24 or 48 h), as detected by western blotting. the expression levels of Erk1/2 and p-Erk1/2 MAPK were significantly reduced in the cell lines in a time-dependent manner following Solamargine treatment, whereas the change in total Erk1/2 MAPK in SGC7901 cells was not significant.

Solamargine purchased from MedChemExpress. Usage Cited in: Int J Oncol. 2019 May;54(5):1545-1554. [Abstract]

Tumor growth in the xenograft mouse model after treatment with Solamargine (10 mg/kg; i.g.; once daily for 8 d). The tumor volume in the Solamargine group was significantly reduced compared with in the control group.
J Food Sci

Identification of Polyphenols, Glycoalkaloids, and Saponins in Solanum scabrum Berries Using HPLC-UV/Vis-MS. [Abstract]

2019 Feb;84(2):235-243. PMID: 30693503
J Sci Food Agric

Quantity assessment of polyphenols, glycoalkaloids and saponins in Solanum scabrum berries of different genetic sources and maturity by HPLC/UV-visible/MS methods. [Abstract]

2019 May;99(7):3578-3587. PMID: 30628085
J Food Drug Anal

Rapid screening of toxic glycoalkaloids and micronutrients in edible nightshades (Solanum spp.). [Abstract]

2018 Apr;26(2):751-760. PMID: 29567246
Oncol Lett

Solamargine induces apoptosis of human renal carcinoma cells via downregulating phosphorylated STAT3 expression. [Abstract]

2023 Sep 28;26(5):493. PMID: 37854861

Solamargine purchased from MedChemExpress. Usage Cited in: Oncol Lett. 2023 Sep 28;26(5):493. [Abstract]

Viability of ACHN and 786-O cells after treatment with SM (Solamargine; 0.1-100.0 μM; 12-72 h). SM inhibited the viability of both cell lines in a concentration- and time-dependent manner.

Solamargine purchased from MedChemExpress. Usage Cited in: Oncol Lett. 2023 Sep 28;26(5):493. [Abstract]

Morphological changes in nuclear chromatin in ACHN and 786-O cells after treatment with SM (Solamargine; 0.05-0.20 μM; 24 h). Nuclear chromatin condensation and fragmentation notably increased with increasing SM concentrations.

Solamargine purchased from MedChemExpress. Usage Cited in: Oncol Lett. 2023 Sep 28;26(5):493. [Abstract]

Western blot analysis of p-STAT3/t-STAT3 in ACHN and 786-O cells after treatment with SM (Solamargine; 0.05-0.20 μM; 24 h). SM reduced the p-STAT3/t-STAT3 ratio in a concentration-dependent manner.

Solamargine purchased from MedChemExpress. Usage Cited in: Oncol Lett. 2023 Sep 28;26(5):493. [Abstract]

Excised tumors, tumor volume, and tumor weight in ACHN-bearing nude mice after treatment with SM (Solamargine; 25 mg/kg; i.g.; once daily for one month). Treatment with SM caused a significant decrease in the volume and weight of tumor tissues compared with the control group.

Solamargine purchased from MedChemExpress. Usage Cited in: Oncol Lett. 2023 Sep 28;26(5):493. [Abstract]

Immunohistochemical staining and quantification with the p-STAT3 antibody in ACHN-bearing nude mice treated with SM (Solamargine; 25 mg/kg; i.g.; once daily). SM treatment significantly decreased the number of p-STAT3⁺ cells compared with the control group.
Eur J Histochem

Solamargine inhibited the progression of non-small cell lung cancer in vitro by activating T cells. [Abstract]

2025 Jun 17;69(3). PMID: 40888491
Rapid Commun Mass Spectrom

Rapid screening of glycoalkaloids in Solanum scabrum and S. nigrum berries using ultra-high-performance liquid chromatography with pathway-specified in-source fragmentation tandem mass spectrometry. [Abstract]

2020 Oct 15;34(19):e8882. PMID: 32644256
bioRxiv

2024 Mar 20.
J Oncol

Solamargine Inhibits Prostate Cancer Cell Growth and Enhances the Therapeutic Efficacy of Docetaxel via Akt Signaling. [Abstract]

2022 Mar 10:2022:9055954. PMID: 35310915

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (115.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.1520 mL

5.7600 mL

11.5199 mL

5 mM

0.2304 mL

1.1520 mL

2.3040 mL

10 mM

0.1152 mL

0.5760 mL

1.1520 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Burger T, et al. Solamargine, a bioactive steroidal alkaloid isolated from Solanum aculeastrum induces non-selective cytotoxicity and P-glycoprotein inhibition. BMC Complement Altern Med. 2018 May 2;18(1):137. [Content Brief]

[2]. Sani IK, et al. Solamargine inhibits migration and invasion of human hepatocellular carcinoma cells through down-regulation of matrix metalloproteinases 2 and 9 expression and activity. Toxicol In Vitro. 2015 Aug;29(5):893-900. [Content Brief]

[3]. Ding X, et al. Induction of apoptosis in human hepatoma SMMC-7721 cells by solamargine from Solanum nigrum L. J Ethnopharmacol. 2012 Jan 31;139(2):599-604. [Content Brief]

[4]. Fu R, et al. Solamargine inhibits gastric cancer progression by regulating the expression of lncNEAT1_2 via the MAPK signaling pathway. Int J Oncol. 2019 May;54(5):1545-1554. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.1520 mL	5.7600 mL	11.5199 mL	28.7999 mL
	5 mM	0.2304 mL	1.1520 mL	2.3040 mL	5.7600 mL
	10 mM	0.1152 mL	0.5760 mL	1.1520 mL	2.8800 mL
	15 mM	0.0768 mL	0.3840 mL	0.7680 mL	1.9200 mL
	20 mM	0.0576 mL	0.2880 mL	0.5760 mL	1.4400 mL
	25 mM	0.0461 mL	0.2304 mL	0.4608 mL	1.1520 mL
	30 mM	0.0384 mL	0.1920 mL	0.3840 mL	0.9600 mL
	40 mM	0.0288 mL	0.1440 mL	0.2880 mL	0.7200 mL
	50 mM	0.0230 mL	0.1152 mL	0.2304 mL	0.5760 mL
	60 mM	0.0192 mL	0.0960 mL	0.1920 mL	0.4800 mL
	80 mM	0.0144 mL	0.0720 mL	0.1440 mL	0.3600 mL
	100 mM	0.0115 mL	0.0576 mL	0.1152 mL	0.2880 mL

Bioz

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Solamargine

Solasonine

Hypericin

Paclitaxel

Homoharringtonine

Tubeimoside I

Geraniin

Pulchinenoside C

Dauricine

Solamargine (Standard)

Ginsenoside F5

Ginsenoside Rg6

Reversan

Publications Citing Use of MedChemExpress (MCE) Solamargine

Biological Activity

Chemical Information

Publications (10)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

Solamargine

Solasonine

Hypericin

Paclitaxel

Homoharringtonine

Tubeimoside I

Geraniin

Pulchinenoside C

Dauricine

Solamargine (Standard)

Ginsenoside F5

Ginsenoside Rg6

Reversan

Publications Citing Use of MedChemExpress (MCE) Solamargine

Biological Activity

Chemical Information

Publications (10)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Solamargine Related Classifications

HY-N0069 Related Classifications

Bioz