Stachyose tetrahydrate

Name: Stachyose tetrahydrate
Brand: MedChemExpress
SKU: HY-113529
Rating: 4.5 (1 reviews)

Cat. No.: HY-113529 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Stachyose tetrahydrate, a functional oligosaccharide, acts as a prebiotic. Stachyose tetrahydrate can prevent indirectly colon cancer cell growth by promoting the proliferation of probiotics or producing beneficial materials in the intestine.

For research use only. We do not sell to patients.

Stachyose tetrahydrate Chemical Structure

CAS No. : 10094-58-3

Size	Price	Stock	Quantity
5 mg	USD 32	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Stachyose tetrahydrate:

Stachyose hydrate In-stock
Stachyose In-stock

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Stachyose tetrahydrate inhibits Caco-2 cell proliferation and induces apoptosis in a dose-dependent manner^[1].
Stachyose highly promotes proliferation of lactic acid bacteria (LAB) by inducing LAB to produce more α-galactosidase to hydrolyze stachyose^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

738.64

Formula

C₂₄H₅₀O₂₅

CAS No.

10094-58-3

Appearance

Solid

Color

White to yellow

SMILES

OC[C@]1(O[C@H](CO)[C@@H](O)[C@@H]1O)O[C@H]2O[C@@H]([C@@H](O)[C@H](O)[C@H]2O)CO[C@H]3O[C@@H]([C@H](O)[C@H](O)[C@H]3O)CO[C@H]4O[C@@H]([C@H](O)[C@H](O)[C@H]4O)CO.O.O.O.O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (338.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (135.38 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3538 mL	6.7692 mL	13.5384 mL
5 mM	0.2708 mL	1.3538 mL	2.7077 mL
10 mM	0.1354 mL	0.6769 mL	1.3538 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (2.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (2.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (2.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (135.38 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.10%

Select Batch:

Data Sheet (280 KB) SDS (0 KB)

COA (259 KB) RP-HPLC (142 KB)

Handling Instructions (2659 KB)

References

[1]. Huang G, et al. Stachyose-induced apoptosis of Caco-2 cells via the caspase-dependent mitochondrial pathway. Food Funct. 2015;6(3):765-771. [Content Brief]

[2]. Pan Q, et al. The Proliferation Mechanism of Lactobacillus plantarum RB1 Stimulated by Stachyose. Curr Microbiol. 2017 Jun;74(6):732-738. [Content Brief]

Animal Administration
^[1]

Mice^[1]

Male Kunming mice in each group (n=8) are administered by intragastric gavage with saline, stachyose (250 mg/kg bw), genistein (100 mg/kg bw), and stachyose (50, 250, and 500 mg/kg bw) together with genistein (100 mg/kg bw) for 4 consecutive weeks, respectively, and then their urine, feces, blood, gut, and liver are collected^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Huang G, et al. Stachyose-induced apoptosis of Caco-2 cells via the caspase-dependent mitochondrial pathway. Food Funct. 2015;6(3):765-771. [Content Brief]

[2]. Pan Q, et al. The Proliferation Mechanism of Lactobacillus plantarum RB1 Stimulated by Stachyose. Curr Microbiol. 2017 Jun;74(6):732-738. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.3538 mL	6.7692 mL	13.5384 mL	33.8460 mL
	5 mM	0.2708 mL	1.3538 mL	2.7077 mL	6.7692 mL
	10 mM	0.1354 mL	0.6769 mL	1.3538 mL	3.3846 mL
	15 mM	0.0903 mL	0.4513 mL	0.9026 mL	2.2564 mL
	20 mM	0.0677 mL	0.3385 mL	0.6769 mL	1.6923 mL
	25 mM	0.0542 mL	0.2708 mL	0.5415 mL	1.3538 mL
	30 mM	0.0451 mL	0.2256 mL	0.4513 mL	1.1282 mL
	40 mM	0.0338 mL	0.1692 mL	0.3385 mL	0.8461 mL
	50 mM	0.0271 mL	0.1354 mL	0.2708 mL	0.6769 mL
	60 mM	0.0226 mL	0.1128 mL	0.2256 mL	0.5641 mL
	80 mM	0.0169 mL	0.0846 mL	0.1692 mL	0.4231 mL
	100 mM	0.0135 mL	0.0677 mL	0.1354 mL	0.3385 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.