1. Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease
  2. VD/VDR Endogenous Metabolite
  3. Vitamin D2

Vitamin D2  (Synonyms: Ergocalciferol; Calciferol; Ercalciol)

Cat. No.: HY-76542 Purity: 98.50%
COA Handling Instructions

Vitamin D2 (Ergocalciferol), drived from plant sources or dietary supplements, could be used as supplement of Vitamin D.

For research use only. We do not sell to patients.

Vitamin D2 Chemical Structure

Vitamin D2 Chemical Structure

CAS No. : 50-14-6

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Vitamin D2:

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Vitamin D2 (Ergocalciferol), drived from plant sources or dietary supplements, could be used as supplement of Vitamin D[1][2].

IC50 & Target

Human Endogenous Metabolite


In Vivo

At sacrifice, after 5 weeks of treatment, chickens receiving omeprazole alone or omeprazole in combination with either restricted food intake or Ergocalciferol weighed 15-25% less (P < 0.01) than chickens given vehicle or receiving reduced amount of food[2].
Combined treatments of fasting and ergocalciferol show more deleterious effects on growth than fasting only or ergocalciferol only and induced face anomalies[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 83 chickens (White Leghorn), weighing 318 ± 9 g[2].
Dosage: 250,000 IU/kg
Administration: S.C. once daily.
Result: Induced a long-lasting hypercalcemia in rat and mice and suppresses the expression of PTH mRNA in the rat.
Clinical Trial
Molecular Weight







White to off-white



Structure Classification
Initial Source

Room temperature in continental US; may vary elsewhere.


-20°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 31.25 mg/mL (78.78 mM; Need ultrasonic)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5211 mL 12.6056 mL 25.2111 mL
5 mM 0.5042 mL 2.5211 mL 5.0422 mL
10 mM 0.2521 mL 1.2606 mL 2.5211 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (5.24 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.08 mg/mL (5.24 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (5.24 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.53%

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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