WAY-262611
Based on 13 publication(s) in Google Scholar
WAY-262611 is a Wnt/β-Catenin agonist that increases bone formation rate with an EC50 of 0.63 μM in TCF-Luciferase assay. WAY-262611 is also a Dkk1 inhibitor.
For research use only. We do not sell to patients.
- Purity: 99.96%
- CAS No.: 1123231-07-1
- Formula: C20H22N4
- Molecular Weight:318.42
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) WAY-262611
More- Nat Biotechnol. 2020 Sep;38(9):1087-1096. [Abstract]
- Nat Commun. 2025 Dec 14. [Abstract]
- Nat Commun. 2025 Nov 27;16(1):10676. [Abstract]
- Int J Biol Sci. 2018 Oct 31;14(13):1923-1934. [Abstract]
- Invest Ophthalmol Vis Sci. 2026 Jan 5;67(1):1. [Abstract]
- Glia. 2023 May;71(5):1197-1216. [Abstract]
- Bone. 2022 Sep:162:116456. [Abstract]
- J Cell Sci. 2019 May 16;132(10):jcs228478. [Abstract]
- Int J Biochem Cell Biol. 2020 Apr:121:105703. [Abstract]
- J Tradit Chin Med. 2025 Aug;45(4):796-805. [Abstract]
- J Recept Signal Transduct Res. 2022 Jun;42(3):268-278. [Abstract]
- Research Square Preprint. 2023 May 17.
- Evid Based Complement Alternat Med. 2019 May 30:2019:9692350. [Abstract]
Customer Validation & Images
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Cell Migration/Invasion Assay
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Flow Cytometry
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Cell Proliferation/Viability Assay
Biological Activity
EC50: 0.63 μM (β-Catenin)[1]
WAY-262611 has the most potent activity in the primary assay, low kinase inhibition potential, and high solubility[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1123231-07-1
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Appearance Solid
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Molecular Weight 318.42
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Formula C20H22N4
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Color Off-white to light yellow
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SMILES
NCC1CCN(C2=NC=CC(C3=CC=C4C=CC=CC4=C3)=N2)CC1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (13)
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Journal Impact Factor
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Most Recent
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Nat Biotechnol
Extending the small-molecule similarity principle to all levels of biology with the Chemical Checker. [Abstract]2020 Sep;38(9):1087-1096. PMID: 32440005 -
Nat Commun
Targeting LRBA triggers CTLA4 degradation and antitumor immunity for cancer immunotherapy. [Abstract]2025 Dec 14. PMID: 41390689 -
Nat Commun
Autocrine ECM molecules establish MSC quiescence during incisor development by disrupting WNT ligand trafficking process. [Abstract]2025 Nov 27;16(1):10676. PMID: 41309624 -
Int J Biol Sci
Saikosaponin A Protects From Pressure Overload-Induced Cardiac Fibrosis via Inhibiting Fibroblast Activation or Endothelial Cell EndMT. [Abstract]2018 Oct 31;14(13):1923-1934. PMID: 30443195 -
Invest Ophthalmol Vis Sci
METTL16 Inhibits Lens Epithelial Cells Function in Diabetic Cataract via m6A-Modified DKK1-Mediated Wnt/β-Catenin Signaling. [Abstract]2026 Jan 5;67(1):1. PMID: 41533902
WAY-262611 purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2026 Jan 5;67(1):1. [Abstract]
Wound healing of HLECs under NG and HG conditions with DMSO or WAY-262611 (0.1 μM) supplement after 12 hours.
WAY-262611 purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2026 Jan 5;67(1):1. [Abstract]
Flow cytometry revealed the cell cycle of HLECs under NG or HG conditions with DMSO or WAY-262611 (0.1 μM) supplement.
WAY-262611 purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2026 Jan 5;67(1):1. [Abstract]
Proliferation of HLECs under NG or HG conditions with DMSO or WAY-262611 (0.1 μM) supplement.
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Glia
Yes-associated protein regulates glutamate homeostasis through promoting the expression of excitatory amino acid transporter-2 in astrocytes via β-catenin signaling. [Abstract]2023 May;71(5):1197-1216. PMID: 36617748 -
Bone
2022 Sep:162:116456. PMID: 35688363 -
J Cell Sci
Dkk1 exacerbates doxorubicin-induced cardiotoxicity by inhibiting the Wnt/β-catenin signaling pathway. [Abstract]2019 May 16;132(10):jcs228478. PMID: 31028181 -
Int J Biochem Cell Biol
Angiotensin II inhibits osteogenic differentiation of isolated synoviocytes by increasing DKK-1 expression. [Abstract]2020 Apr:121:105703. PMID: 32014499 -
J Tradit Chin Med
Mechanism of electroacupuncture involve in lens-induced myopia guinea pigs by inhibiting wnt/β-catenin signaling pathway. [Abstract]2025 Aug;45(4):796-805. PMID: 40810225 -
J Recept Signal Transduct Res
MicroRNA-320a-containing exosomes from human umbilical cord mesenchymal stem cells curtail proliferation and metastasis in lung cancer by binding to SOX4. [Abstract]2022 Jun;42(3):268-278. PMID: 34096448 -
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Evid Based Complement Alternat Med
Antimetastasis Effect of Astragalus membranaceus- Curcuma zedoaria via β-Catenin Mediated CXCR4 and EMT Signaling Pathway in HCT116. [Abstract]2019 May 30:2019:9692350. PMID: 31275425
Solvent & Solubility
DMSO : 40 mg/mL (125.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (5.24 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (5.24 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Rats: WAY-262611 is dissolved in DMSO and diluted with saline for iv (Rats). WAY-262611 is prepared in 0.5% methylcellulose/2% Tween-80 for po OVX rats14 are treated orally with 5 (po, vehicle=0.5% methylcellulose/2% Tween-80, qd, 28 days) at four doses. Trabecular bone formation rate (BFR) in the tibia is established in all dose groups at the end of the in-life portion of the study. A clear dose response and activity as low as 0.3 mg/kg/day are observed[1].
Mice: To confirm activity via the Wnt pathway, the calvariae of wild type (wt) and Dkk-1 knockout (KO) mice are treated with 5 once a day for 7 days (DMSO solution, sc injection). The KO animals are not expected to respond because of the inherent inability to inhibit a missing target protein, while wild type animals with fully expressed Dkk-1 are expected to show a pharmacological response [1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Pelletier JC, et al. (1-(4-(Naphthalen-2-yl)pyrimidin-2-yl)piperidin-4-yl)methanamine: a wingless beta-catenin agonist that increases bone formation rate. J Med Chem. 2009 Nov 26;52(22):6962-5. [Content Brief]
[2]. L Enochson, et al. GDF5 reduces MMP13 expression in human chondrocytes via DKK1 mediated canonical Wnt signaling inhibition. Osteoarthritis Cartilage. 2014 Apr;22(4):566-77. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1405 mL | 15.7025 mL | 31.4051 mL | 78.5127 mL |
| 5 mM | 0.6281 mL | 3.1405 mL | 6.2810 mL | 15.7025 mL | |
| 10 mM | 0.3141 mL | 1.5703 mL | 3.1405 mL | 7.8513 mL | |
| 15 mM | 0.2094 mL | 1.0468 mL | 2.0937 mL | 5.2342 mL | |
| 20 mM | 0.1570 mL | 0.7851 mL | 1.5703 mL | 3.9256 mL | |
| 25 mM | 0.1256 mL | 0.6281 mL | 1.2562 mL | 3.1405 mL | |
| 30 mM | 0.1047 mL | 0.5234 mL | 1.0468 mL | 2.6171 mL | |
| 40 mM | 0.0785 mL | 0.3926 mL | 0.7851 mL | 1.9628 mL | |
| 50 mM | 0.0628 mL | 0.3141 mL | 0.6281 mL | 1.5703 mL | |
| 60 mM | 0.0523 mL | 0.2617 mL | 0.5234 mL | 1.3085 mL | |
| 80 mM | 0.0393 mL | 0.1963 mL | 0.3926 mL | 0.9814 mL | |
| 100 mM | 0.0314 mL | 0.1570 mL | 0.3141 mL | 0.7851 mL |
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