Z-Gly-Gly-Arg-AMC
Based on 11 publication(s) in Google Scholar
Z-Gly-Gly-Arg-AMC is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm).
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 66216-78-2
- Formula: C28H33N7O7
- Molecular Weight:579.60
-
Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Z-Gly-Gly-Arg-AMC
More- Cell Rep Med. 2025 Aug 19;6(8):102260. [Abstract]
- Acta Pharmacol Sin. 2024 Nov;45(11):2300-2312. [Abstract]
- Int J Biol Macromol. 2019 Sep 15:137:261-269. [Abstract]
- Int J Biol Macromol. 2019 Aug 1:134:622-630. [Abstract]
- Blood Adv. 2025 Jun 10;9(11):2695-2705. [Abstract]
- J Mater Chem B. 2017 Oct 14;5(38):7876-7884. [Abstract]
- RSC Adv. 2020 Jan 22;10(7):3626-3635. [Abstract]
- Chem Biol Interact. 2023 Jun 1:378:110501. [Abstract]
- Bioorg Chem. 2019 Nov:92:103199. [Abstract]
- Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jan 5:264:120250. [Abstract]
- Phytochemistry. 2019 Sep:165:112025. [Abstract]
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation[3]
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 10 mM.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 10 μM with PBS (or other buffer) (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Thrombin inhibition screen
2.1 The samples and thrombin are transferred to a 384-well plate in sequence.
2.2 Add Z-Gly-Gly-Arg-AMC (50 μM) after incubation for 15 minutes.
2.3 Using a buffer containing 10 mM Tris-Cl (pH 7.4), 150 mM NaCl, 10 mM MgCl2, 1 mM CaCl2, 0.1% w/v BSA, 0.01% v/v Triton-X100, and 1% v/v DMSO and the fluorogenic substrate Z-Gly-Gly-Arg-AMC (50 μM).
2.4 The fluorescence intensity is measured with a fluorescence plate reader (Ex/Em=360/465 nm) at 25°C for a period of 30 min with a read every 3 min.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
480
390
Chemical Information
-
CAS No. 66216-78-2
-
Appearance Solid
-
Molecular Weight 579.60
-
Formula C28H33N7O7
-
Color White to off-white
-
Sequence
Z-Gly-Gly-{Arg-AMC}
-
Sequence Shortening
ZGG-{Arg-AMC}
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (11)
-
Journal Impact Factor
-
Most Recent
-
Cell Rep Med
High-fat diet increases circulating palmitic acid produced by gut Bacteroides thetaiotaomicron to promote thrombosis. [Abstract]2025 Aug 19;6(8):102260. PMID: 40749683 -
Acta Pharmacol Sin
Lethal pulmonary thromboembolism in mice induced by intravenous human umbilical cord mesenchymal stem cell-derived large extracellular vesicles in a dose- and tissue factor-dependent manner. [Abstract]2024 Nov;45(11):2300-2312. PMID: 38914677 -
Int J Biol Macromol
Discovery of a highly specific and efficacious inhibitor of human carboxylesterase 2 by large-scale screening. [Abstract]2019 Sep 15:137:261-269. PMID: 31260759 -
Int J Biol Macromol
Natural constituents of St. John's Wort inhibit the proteolytic activity of human thrombin. [Abstract]2019 Aug 1:134:622-630. PMID: 31047931 -
Blood Adv
Development of a protease-resistant ADAMTS13 to improve stability against proteolytic degradation. [Abstract]2025 Jun 10;9(11):2695-2705. PMID: 40127394 -
J Mater Chem B
2,3-Dialdehyde nanofibrillated cellulose as a potential material for the treatment of MRSA infection. [Abstract]2017 Oct 14;5(38):7876-7884. PMID: 32264389 -
RSC Adv
Inhibition of human thrombin by the constituents of licorice: inhibition kinetics and mechanistic insights through in vitro and in silico studies. [Abstract]2020 Jan 22;10(7):3626-3635. PMID: 35492646 -
Chem Biol Interact
Discovery of seven-membered ring berberine analogues as highly potent and specific hCES2A inhibitors. [Abstract]2023 Jun 1:378:110501. PMID: 37080375 -
Bioorg Chem
2019 Nov:92:103199. PMID: 31446241 -
Spectrochim Acta A Mol Biomol Spectrosc
An online, non-destructive method for simultaneously detecting chemical, biological, and physical properties of herbal injections using hyperspectral imaging with artificial intelligence. [Abstract]2022 Jan 5:264:120250. PMID: 34391991 -
Phytochemistry
Anthraquinones from Cassiae semen as thrombin inhibitors: in vitro and in silico studies. [Abstract]2019 Sep:165:112025. PMID: 31207449
Solvent & Solubility
DMSO : 100 mg/mL (172.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
-
Handling Instructions (2659 KB)
References
[1]. Tarandovskiy ID, et al. Antiplatelet agents can promote two-peaked thrombin generation in platelet rich plasma: mechanism and possible applications. PLoS One. 2013;8(2):e55688. [Content Brief]
[2]. Machlus KR, et al. Effects of tissue factor, thrombomodulin and elevated clotting factor levels on thrombin generation in the calibrated automated thrombogram. Thromb Haemost. 2009 Nov;102(5):936-44. [Content Brief]
[3]. Mothukuri GK, et al. Macrocycle synthesis strategy based on step-wise "adding and reacting" three components enables screening of large combinatorial libraries. Chem Sci. 2020 Jun 26;11(30):7858-7863. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7253 mL | 8.6266 mL | 17.2533 mL | 43.1332 mL |
| 5 mM | 0.3451 mL | 1.7253 mL | 3.4507 mL | 8.6266 mL | |
| 10 mM | 0.1725 mL | 0.8627 mL | 1.7253 mL | 4.3133 mL | |
| 15 mM | 0.1150 mL | 0.5751 mL | 1.1502 mL | 2.8755 mL | |
| 20 mM | 0.0863 mL | 0.4313 mL | 0.8627 mL | 2.1567 mL | |
| 25 mM | 0.0690 mL | 0.3451 mL | 0.6901 mL | 1.7253 mL | |
| 30 mM | 0.0575 mL | 0.2876 mL | 0.5751 mL | 1.4378 mL | |
| 40 mM | 0.0431 mL | 0.2157 mL | 0.4313 mL | 1.0783 mL | |
| 50 mM | 0.0345 mL | 0.1725 mL | 0.3451 mL | 0.8627 mL | |
| 60 mM | 0.0288 mL | 0.1438 mL | 0.2876 mL | 0.7189 mL | |
| 80 mM | 0.0216 mL | 0.1078 mL | 0.2157 mL | 0.5392 mL | |
| 100 mM | 0.0173 mL | 0.0863 mL | 0.1725 mL | 0.4313 mL |
Z-Gly-Gly-Arg-AMC Related Classifications
HY-P0019 Related Classifications
Powered by Bioz