Cat. No.: HY-N6012

(-)-Alkannin

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

(-)-Alkannin, found in Alkanna tinctoria, is used as a food coloring. (-)-Alkannin shows anticancer activity, arrests cell cycle, and induces apoptosis. (-)-Alkannin improves hepatic inflammation in a Rho-kinase pathway.

For research use only. We do not sell to patients.

Purity: 99.88%
CAS No.: 517-88-4
Formula: C₁₆H₁₆O₅
Molecular Weight:288.30

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

(-)-Alkannin Chemical Structure

CAS No.: 517-88-4

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
AU565	IC₅₀	0.9 μM Compound: 4	Cytotoxicity against human AU565 cells after 48 hrs by MTT assay Cytotoxicity against human AU565 cells after 48 hrs by MTT assay	[PMID: 20405844]
DU-145	IC₅₀	19.9 μM Compound: AK	Cytotoxicity against human DU145 cells by MTT assay Cytotoxicity against human DU145 cells by MTT assay	[PMID: 25127868]
DU-145	IC₅₀	19.9 μM Compound: Alkannin	Anticancer activity against human DU-145 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Anticancer activity against human DU-145 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35367708]
DU-145	IC₅₀	5.02 μM Compound: 4	Cytotoxicity against human DU145 cells after 48 hrs by MTT assay Cytotoxicity against human DU145 cells after 48 hrs by MTT assay	[PMID: 20405844]
Fibroblast	IC₅₀	1.5 μM Compound: AK	Cytotoxicity against human skin fibroblast cells by MTT assay Cytotoxicity against human skin fibroblast cells by MTT assay	[PMID: 25127868]
Fibroblast	IC₅₀	1.5 μM Compound: Alkannin	Cytotoxicity against human skin fibroblast cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human skin fibroblast cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35367708]
Fibroblast	IC₅₀	1.8 μM Compound: Alkannin	Cytotoxicity against human skin fibroblast cells incubated for 24 hrs by MTT assay Cytotoxicity against human skin fibroblast cells incubated for 24 hrs by MTT assay	[PMID: 35367708]
HCT-15	IC₅₀	1.11 μM Compound: Alkannin	Anticancer activity against human HCT-15 cells assessed as reduction in cell viability for 72 hrs by MTT assay Anticancer activity against human HCT-15 cells assessed as reduction in cell viability for 72 hrs by MTT assay	[PMID: 35367708]
HeLa	IC₅₀	0.91 μM Compound: 4	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 20405844]
Hep 3B2	IC₅₀	3.84 μM Compound: 4	Cytotoxicity against human Hep3B cells after 48 hrs by MTT assay Cytotoxicity against human Hep3B cells after 48 hrs by MTT assay	[PMID: 20405844]
HepG2	IC₅₀	3.8 μM Compound: 4	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 20405844]
HT-29	IC₅₀	4.03 μM Compound: 4	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 20405844]
K562	IC₅₀	1.3 μM Compound: AK	Cytotoxicity against human K562 cells by MTT assay Cytotoxicity against human K562 cells by MTT assay	[PMID: 25127868]
K562	IC₅₀	1.3 μM Compound: Alkannin	Anticancer activity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Anticancer activity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35367708]
K562	IC₅₀	1.5 μM Compound: Alkannin	Anticancer activity against human K562 cells assessed as reduction in cell viability for 72 hrs by MTT assay Anticancer activity against human K562 cells assessed as reduction in cell viability for 72 hrs by MTT assay	[PMID: 35367708]
LNCaP	IC₅₀	10.74 μM Compound: 4	Cytotoxicity against human LNCAP cells after 48 hrs by MTT assay Cytotoxicity against human LNCAP cells after 48 hrs by MTT assay	[PMID: 20405844]
MCF7	IC₅₀	2.4 μM Compound: AK	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 25127868]
MCF7	IC₅₀	2.4 μM Compound: Alkannin	Anticancer activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Anticancer activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35367708]
MCF7	IC₅₀	2.62 μM Compound: Alkannin	Anticancer activity against human MCF7 cells assessed as reduction in cell viability for 72 hrs by MTT assay Anticancer activity against human MCF7 cells assessed as reduction in cell viability for 72 hrs by MTT assay	[PMID: 35367708]
MCF7	IC₅₀	3.06 μM Compound: 4	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 20405844]
MDA-MB-231	IC₅₀	4.27 μM Compound: 4	Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay	[PMID: 20405844]
NIH3T3	IC₅₀	5.21 μM Compound: 4	Cytotoxicity against mouse 3T3 cells after 48 hrs by MTT assay Cytotoxicity against mouse 3T3 cells after 48 hrs by MTT assay	[PMID: 20405844]
PC-3	IC₅₀	3.25 μM Compound: 4	Cytotoxicity against human PC3 cells after 48 hrs by MTT assay Cytotoxicity against human PC3 cells after 48 hrs by MTT assay	[PMID: 20405844]
SAOS-2	IC₅₀	2.31 μM Compound: 4	Cytotoxicity against human Saos2 cells after 48 hrs by MTT assay Cytotoxicity against human Saos2 cells after 48 hrs by MTT assay	[PMID: 20405844]
SK-BR-3	IC₅₀	0.78 μM Compound: 4	Cytotoxicity against human SKBR3 cells after 48 hrs by MTT assay Cytotoxicity against human SKBR3 cells after 48 hrs by MTT assay	[PMID: 20405844]
Vero	IC₅₀	0.7 μM Compound: 4	Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay	[PMID: 20405844]

In Vitro

(-)-Alkannin inhibits HCT-116 and SW-480 cells growth with IC₅₀s of 2.38 and 4.53 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 517-88-4
Appearance Solid
Molecular Weight 288.30
Formula C₁₆H₁₆O₅
Color Brown to red
SMILES

OC1=C2C(C(C=C([C@H](C/C=C(C)/C)O)C2=O)=O)=C(O)C=C1
Structure Classification
- Alkaloids
- Other Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (2)

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Phytomedicine

Alkannin triggered apoptosis and ferroptosis in gastric cancer by suppressing lipid metabolism mediated by the c-Fos/SREBF1 axis. [Abstract]

2025 May:140:156604. PMID: 40049103
Drug Des Devel Ther

Alkannin Induces G2/M-Phase Arrest, Apoptosis, and Inhibition of Invasion by Targeting GSK3β in Esophageal Squamous Cell Carcinoma. [Abstract]

2024 Nov 25:18:5377-5395. PMID: 39618426

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (115.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.4686 mL

17.3430 mL

34.6861 mL

5 mM

0.6937 mL

3.4686 mL

6.9372 mL

10 mM

0.3469 mL

1.7343 mL

3.4686 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hosokawa Y, et al. Alkannin inhibits CCL3 and CCL5 production in human periodontal ligament cells. Cell Biol Int. 2016 Dec;40(12):1380-1385. [Content Brief]

[2]. Huu Tung N, et al. Naphthoquinone components from Alkanna tinctoria (L.) Tausch show significant antiproliferative effects on human colorectal cancer cells. Phytother Res. 2013 Jan;27(1):66-70. [Content Brief]

[3]. Xue W, et al. Alkannin Inhibited Hepatic Inflammation in Diabetic Db/Db Mice. Cell Physiol Biochem. 2018;45(6):2461-2470. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.4686 mL	17.3430 mL	34.6861 mL	86.7152 mL
	5 mM	0.6937 mL	3.4686 mL	6.9372 mL	17.3430 mL
	10 mM	0.3469 mL	1.7343 mL	3.4686 mL	8.6715 mL
	15 mM	0.2312 mL	1.1562 mL	2.3124 mL	5.7810 mL
	20 mM	0.1734 mL	0.8672 mL	1.7343 mL	4.3358 mL
	25 mM	0.1387 mL	0.6937 mL	1.3874 mL	3.4686 mL
	30 mM	0.1156 mL	0.5781 mL	1.1562 mL	2.8905 mL
	40 mM	0.0867 mL	0.4336 mL	0.8672 mL	2.1679 mL
	50 mM	0.0694 mL	0.3469 mL	0.6937 mL	1.7343 mL
	60 mM	0.0578 mL	0.2891 mL	0.5781 mL	1.4453 mL
	80 mM	0.0434 mL	0.2168 mL	0.4336 mL	1.0839 mL
	100 mM	0.0347 mL	0.1734 mL	0.3469 mL	0.8672 mL

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(-)-Alkannin

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Natural Product Library

Anti-Cancer Compound Library

Traditional Chinese Medicine Active Compound Library

Alkaloids Library

Anti-Cancer Natural Product Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Cell Death Library

Bioactive Compound Library Max

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Metabolite Library

Mongolian Medicine Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) (-)-Alkannin

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

(-)-Alkannin

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Natural Product Library

Anti-Cancer Compound Library

Traditional Chinese Medicine Active Compound Library

Alkaloids Library

Anti-Cancer Natural Product Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Cell Death Library

Bioactive Compound Library Max

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Metabolite Library

Mongolian Medicine Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) (-)-Alkannin

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

(-)-Alkannin Related Classifications

Cat. No.: HY-N6012