ALKBH5-IN-4
Based on 1 Customer Validation
ALKBH5-IN-4 (compound 3) is a RNA m6A demethylase ALKBH5 inhibitor with an IC50 of 0.84 μM. ALKBH5-IN-4 shows inhibitory effect on leukemia cells[1].
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- Purity: 98.82%
- CAS No.: 37510-29-5
- 화학식: C10H10O4S
- 분자량:226.25
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보관:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CCRF-CEM | IC50 |
1.38 μM
Compound: 120
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Cytotoxicity against human CCRF-CEM cells assessed as reduction in cell viability incubated for 48 hrs
Cytotoxicity against human CCRF-CEM cells assessed as reduction in cell viability incubated for 48 hrs
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[PMID: 34792334] |
| HL-60 | IC50 |
1.38 μM
Compound: 120
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Cytotoxicity against human HL-60 cells assessed as reduction in cell viability incubated for 48 hrs
Cytotoxicity against human HL-60 cells assessed as reduction in cell viability incubated for 48 hrs
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[PMID: 34792334] |
| K562 | IC50 |
1.38 μM
Compound: 120
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Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs
Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs
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[PMID: 34792334] |
ALKBH5-IN-4 (1-100 μM, 0-48 h) inhibits HEK-293T, CCRF-CEM, HL-60, Jurkat, K562, A-172 cells proliferation with IC50 s of >50, 1.38, 11.9, 47.8, 16.5, >50 μM, respectively[1].
ALKBH5-IN-4 (1-100 μM, 2 h) inhibits the RNA m6A demethylase ALKBH5 with anIC50 of 0.84 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK-293T, CCRF-CEM, HL-60, Jurkat, K562, A-172 cells
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Concentration:1, 10, 100 μM
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Incubation Time:4, 8, 24, 48 h
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Result:Inhibited HEK-293T, CCRF-CEM, HL-60, Jurkat, K562, A-172 cells proliferation respectively.
Chemical Information
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CAS No. 37510-29-5
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Appearance Solid
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분자량 226.25
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화학식 C10H10O4S
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Color White to off-white
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SMILES
O=C(O)CSC(O)C(C1=CC=CC=C1)=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
용액&용해도
DMSO : 50 mg/mL (220.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.05 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.05 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.4199 mL | 22.0994 mL | 44.1989 mL | 110.4972 mL |
| 5 mM | 0.8840 mL | 4.4199 mL | 8.8398 mL | 22.0994 mL | |
| 10 mM | 0.4420 mL | 2.2099 mL | 4.4199 mL | 11.0497 mL | |
| 15 mM | 0.2947 mL | 1.4733 mL | 2.9466 mL | 7.3665 mL | |
| 20 mM | 0.2210 mL | 1.1050 mL | 2.2099 mL | 5.5249 mL | |
| 25 mM | 0.1768 mL | 0.8840 mL | 1.7680 mL | 4.4199 mL | |
| 30 mM | 0.1473 mL | 0.7366 mL | 1.4733 mL | 3.6832 mL | |
| 40 mM | 0.1105 mL | 0.5525 mL | 1.1050 mL | 2.7624 mL | |
| 50 mM | 0.0884 mL | 0.4420 mL | 0.8840 mL | 2.2099 mL | |
| 60 mM | 0.0737 mL | 0.3683 mL | 0.7366 mL | 1.8416 mL | |
| 80 mM | 0.0552 mL | 0.2762 mL | 0.5525 mL | 1.3812 mL | |
| 100 mM | 0.0442 mL | 0.2210 mL | 0.4420 mL | 1.1050 mL |
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