Alnuctamab  (Synonyms: EM901; CC-93269)

Alnuctamab (EM901) is a bispecific T-cell engager targeting BCMA and CD3, belonging to an asymmetric two-armed humanized IgG1 antibody. Alnuctamab can simultaneously bind to BCMA expressed on the surface of myeloma cells and CD3 molecules on the surface of T cells, recruiting T cells to kill tumors. Alnuctamab is used for the research of multiple myeloma. Recommend Isotype Controls: Human IgG1 kappa, Isotype Control (HY-P99001)^[1][2][3].

IgG1-kappa/lambda with domain crossover, trivalent

Human IgG1 kappa, Isotype Control

Human

BCMA & CD3

Alnuctamab (72 h), when co-cultured with T cells and BCMA⁺ myeloma cell lines (H929, OPM-2, L363), in combination with CELMoD drugs (Pomalidomide (HY-10984), Mezigdomide (HY-129395) and Iberdomide (HY-101291)), enhances T cell-mediated tumor cell killing efficacy, as demonstrated by reduced IC₅₀ values and increased clearance rate of multiple myeloma cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Alnuctamab, when combined with Mezigdomide (HY-129395) in immunodeficient mice implanted with H929 myeloma cells, significantly inhibits tumor growth, enhances the infiltration of T cells (especially CD8⁺ cells) into tumors, and increases the expression of T cell activation markers (such as CD69) within tumors^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

916  [NCBI] & 931  [NCBI]

P07766 & P11836

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

192.13 kDa

2296827-07-9

Liquid

Colorless to light yellow

[Alnuctamab]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  IgG1-kappa/lambda with domain crossover, trivalent

• 
  Immobilized CD3 epsilon/CD3e Protein can bind Alnuctamab. The EC₅₀ for this effect is 0.728 ng/mL.
• 
  Immobilized BCMA/TNFRSF17 Protein, Human can bind Alnuctamab. The EC₅₀ for this effect is 0.051 ng/mL.
• 
  Flow Cytometry analysis of K562 cells labelling BCMA (red) with Alnuctamab (HY-P99430). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 11,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Paiva B, et al. Synergistic antitumor activity of alnuctamab (ALNUC; BMS-986349; CC-93269), a BCMA 2+ 1 T cell engager (TCE), and celmod agents in multiple myeloma (MM) preclinical models. Blood, 2022, 140(Supplement 1): 7054-7055.

  [2]. Wong S W, et al. Alnuctamab (ALNUC; BMS-986349; CC-93269), a B-cell maturation antigen (BCMA) x CD3 T-cell engager (TCE), in patients (pts) with relapsed/refractory multiple myeloma (RRMM): results from a phase 1 first-in-human clinical study[J]. Blood, 2022, 140(Supplement 1): 400-402.

  [3]. Danny V Jeyaraju, et al. Suppression of Myeloid Cell-Derived Proinflammatory Cytokines with Celmod Agents: Implications for CRS with T-Cell Engagers (TCEs). Blood (2022) 140 (Supplement 1): 7070–7071.