2. Autophagy PI3K/Akt/mTOR Epigenetics
  3. Autophagy AMPK
  4. AMDE-1

AMDE-1 is a potent autophagy inducer. AMDE-1 induces autophagy by the AMPK-mTORC1-ULK1 pathway and at the same time inhibited autophagy-mediated degradation by causing lysosome dysfunction. AMDE-1 can be used in research of cancer.

For research use only. We do not sell to patients.

AMDE-1 Chemical Structure

AMDE-1 Chemical Structure

CAS No. : 478043-30-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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 USD 275 In-stock
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10 mM * 1 mL in DMSO USD 275 In-stock
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5 mg USD 250 In-stock
10 mg USD 380 In-stock
25 mg USD 685 In-stock
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100 mg USD 1330 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

AMDE-1 is a potent autophagy inducer. AMDE-1 induces autophagy by the AMPK-mTORC1-ULK1 pathway and at the same time inhibited autophagy-mediated degradation by causing lysosome dysfunction. AMDE-1 can be used in research of cancer[1].

Molecular Weight

415.72

Formula

C18H8ClF6N3
CAS No.
Appearance

Solid

Color

Orange to red

SMILES

N#CC(C1=NC2=NC(C(F)(F)F)=CC(C(F)(F)F)=C2C=C1)C3=CC=CC(Cl)=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (240.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4055 mL 12.0273 mL 24.0547 mL
5 mM 0.4811 mL 2.4055 mL 4.8109 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 2.5 mg/mL (6.01 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4055 mL 12.0273 mL 24.0547 mL 60.1366 mL
5 mM 0.4811 mL 2.4055 mL 4.8109 mL 12.0273 mL
10 mM 0.2405 mL 1.2027 mL 2.4055 mL 6.0137 mL
15 mM 0.1604 mL 0.8018 mL 1.6036 mL 4.0091 mL
20 mM 0.1203 mL 0.6014 mL 1.2027 mL 3.0068 mL
25 mM 0.0962 mL 0.4811 mL 0.9622 mL 2.4055 mL
30 mM 0.0802 mL 0.4009 mL 0.8018 mL 2.0046 mL
40 mM 0.0601 mL 0.3007 mL 0.6014 mL 1.5034 mL
50 mM 0.0481 mL 0.2405 mL 0.4811 mL 1.2027 mL
60 mM 0.0401 mL 0.2005 mL 0.4009 mL 1.0023 mL
80 mM 0.0301 mL 0.1503 mL 0.3007 mL 0.7517 mL
100 mM 0.0241 mL 0.1203 mL 0.2405 mL 0.6014 mL
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AMDE-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AMDE-1478043-30-0AMDE1AMDE 1AutophagyAMPKAMP-activated protein kinaseInhibitorinhibitorinhibit

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