Ancitabine  (Synonyms: Cyclocytidine; Cyclo-CMP)

Ancitabine (Cyclocytidine) is a cytarabine derivative that inhibits viral replication. Ancitabine blocks vaccinia virus DNA replication, the progression of viral protein synthesis from early to late stages, and one-step growth of vaccinia virus. Ancitabine is applicable to research related to vaccinia virus infection, leukemia, human cytomegalovirus infection and colorectal cancer^[1][2][3].

Ancitabine non-toxically inhibits vaccinia virus replication in BSC40 African green monkey kidney cells with a 48 h IC₉₉ of 11 μM, acting by blocking viral DNA replication and preventing progression from early to late viral protein synthesis^[1].
Ancitabine (5 μg/mL; 2 days) completely inhibits replication of the KOS strain of HSV-1 in HEL cells^[2].
Ancitabine (5 μg/mL; 2 days) completely inhibits replication of the Savage strain of HSV-2 in HEL cells^[2].
Ancitabine (0.14-10 μg/mL; 4 days) potently inhibits replication of the AD169 strain of HCMV in HEL cells, with an ED₉₀ of 0.14 μg/mL and complete inhibition at 10 μg/mL maintained for 4 days^[2].
Ancitabine (<0.010->100 μg/mL; 4 days) is non-toxic to stationary HEL cells at concentrations up to 100 μg/mL, but is highly cytotoxic to growing HEL cells with a CyD₅₀ of <0.010 μg/mL^[2].
Ancitabine (500 μM; 48 h) is converted to its triphosphate form in HCMV-infected HEL cells^[2].
Ancitabine (5 μg/mL; 48 h) substantially suppresses synthesis of HCMV DNA in infected HEL cells^[2].
Ancitabine (10 μM; 72 h) induces intracellular vesicle formation in human colorectal carcinoma HCT116 cells with reproducible morphological profiles^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ancitabine is rapidly excreted in the urine of rhesus monkeys, Beagle dogs, and Sprague-Dawley rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

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• [1]. Deng L, et al. Identification of novel antipoxviral agents: mitoxantrone inhibits vaccinia virus replication by blocking virion assembly. J Virol. 2007;81(24):13392-13402.  [Content Brief]

  [2]. Nakamura K, et al. Antiviral effect of antileukemic drugs N4-behenoyl-1-beta-D-arabinofuranosylcytosine (BH-AC) and 2,2'-anhydro-1-beta-D-arabinofuranosylcytosine (cyclo-C) against human cytomegalovirus. J Med Virol. 1990;31(2):141-147.  [Content Brief]

  [3]. Aftab O, et al. Detection of cell aggregation and altered cell viability by automated label-free video microscopy: a promising alternative to endpoint viability assays in high-throughput screening. J Biomol Screen. 2015;20(3):372-381.  [Content Brief]