Anti-Mouse/Human CD11b Antibody (M1/70)
Based on 1 publication(s) in Google Scholar
Anti-Mouse/Human CD11b Antibody (M1/70) is an anti-mouse CD11b IgG2b monoclonal antibody. Anti-Mouse/Human CD11b Antibody (M1/70) can significantly inhibit the adhesion between dendritic cells (DCs) and platelets. Anti-Mouse/Human CD11b Antibody (M1/70) can kill ovarian cancer cells and inhibit their migration. Anti-Mouse/Human CD11b Antibody (M1/70) can alleviate renal fibrosis and inflammation. Anti-Mouse/Human CD11b Antibody (M1/70) can be used for researches on inflammation conditions and cancer such as ischemia-reperfusion injury (IRI), thrombotic inflammatory conditions and ovarian cancer.
For research use only. We do not sell to patients.
- Purity: 95.00%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Anti-Mouse/Human CD11b Antibody (M1/70)
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Biological Activity
Description
Isotype
Rat IgG2b kappa
Recommend Isotype Controls
Species Reactivity
Mouse
In Vitro
Anti-Mouse/Human CD11b Antibody (M1/70) (10 μg/mL, 30 min) significantly inhibits the adhesion of bone marrow-derived dendritic cells (bmDCs) to fixed platelets[2].
Anti-Mouse/Human CD11b Antibody (M1/70) (5-10 μg/mL, 24 h) significantly kills epithelial ovarian cancer (EOC) cells[3].
Anti-Mouse/Human CD11b Antibody (M1/70) (5-15 μg/mL, 36 h) dose dependently inhibits cell migration in SKOV-3 and MDAH-2774 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A2780, MDAH-2774, SKOV-3, OV90, TOV-21G, TOV112D and OV433 cells
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Concentration:5 and 10 μg/mL
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Incubation Time:24 h
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Result:Significantly killed EOC cells with various efficacies ranging from 27 % to 96 %.
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Cell Line:SKOV-3 and MDAH-2774 cells
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Concentration:5, 10 and 15 μg/mL
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Incubation Time:36 h
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Result:Delayed closure until 28 hours at 10 μg/mL, inhibited 50 % migration at 15 μg/mL.
In Vivo
Anti-Mouse/Human CD11b Antibody (M1/70) (50 μg, i.v., single dose) significantly inhibits the adhesion of DCs to damaged blood vessels in wild type C57BL/6 mice with a carotid artery ligation model[2].
Anti-Mouse/Human CD11b Antibody (M1/70) (60 μg, intratumoral injection, once daily, for 5 days) significantly inhibits tumor growth in female anthymic Nude-Foxn1nu mice bearing SKOV-3 tumors[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male C57BL/6J mice (6-8 weeks) with an IRI model[1]
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Dosage:2.5 mg/kg
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Administration:Intraperitoneal injection (i.p.), once every 2 days for 14 days
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Result:Improved renal fibrosis.
Reduced the the level of Col1a1, Fn1, α-SMA and Col3a1.
Improved the pathological progression of severe acute kidney injury (AKI) to chronic kidney disease (CKD).
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Animal Model:Wild type C57BL/6 mice with a carotid artery ligation model[2]
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Dosage:50 μg
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Administration:Intravenous injection (i.v.), single dose
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Result:Significantly inhibited the adhesion of DCs to damaged blood vessels.
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Animal Model:1.5×106 SKOV-3 cells injected female anthymic Nude-Foxn1nu mice[3]
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Dosage:60 μg
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Administration:Intratumoral injection, once daily for 5 days
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Result:Reduced tumor volume by 47 % on day 2, reduced tumor volume by 64.9 % on day 4 and reduced tumor volume by 59.7 % on day 5.
Had no significant systemic toxicity.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vivo CD11b neutralization; ILC2 cell purification; Flow cytometry
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse/Human CD11b Antibody (M1/70)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
Purity & Documentation
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Data Sheet (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Zhou H, et al. CD11b deficiency attenuates the ischemia/reperfusion-induced AKI-to-CKD process by regulating macrophage polarization. FASEB J. 2024 Dec 15;38(23):e70216. [Content Brief]
[2]. Nording H, et al. Activated Platelets Upregulate β2 Integrin Mac-1 (CD11b/CD18) on Dendritic Cells, Which Mediates Heterotypic Cell-Cell Interaction. J Immunol. 2022 Apr 1;208(7):1729-1741. [Content Brief]
[3]. Saed GM, et al. Novel expression of CD11b in epithelial ovarian cancer: Potential therapeutic target. Gynecol Oncol. 2018 Mar;148(3):567-575. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse/Human CD11b Antibody (M1/70) Related Classifications
HY-P99121 Related Classifications
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