Art. -Nr.: HY-122122

ML-60218

11 Cited Publications

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Based on 11 publication(s) in Google Scholar

ML-60218 is a broad-spectrum RNA pol III inhibitor, with IC₅₀s of 32 and 27 μM for Saccharomyces cerevisiae and human. ML-60218 disrupts already assembled viroplasms and to hamper the formation of new ones without the need for de novo transcription of cellular RNAs.

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Reinheit: 99.69%
CAS. Nr.: 577784-91-9
Formel: C₁₉H₁₅Cl₂N₃O₂S₂
Molecular Weight:452.38

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

ML-60218 Chemical Structure

CAS. Nr.: 577784-91-9

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Publications (11)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen :

In Vitro

Combination of SAHA and ML-60218 produces enhanced suppression of proliferation in human pancreatic adenocarcinoma by impairing cell cycle progression and inducing apoptosis. ML-60218 reverses SAHA-stimulated tRNA expression in PANC-1 and BxPC-3 cells. ML-60218 enhances the ability of HDAC inhibitors to induce apoptosis and cell cycle arrest^[2].
In in vitro transcription assays with purified double-layered particles (DLPs), ML-60218 shows dose-dependent inhibitory activity, indicating the viral nature of its target. ML-60218 is found to interfere with the formation of higher-order structures of VP6, the protein forming the DLP outer layer, without compromising its ability to trimerize. Electron microscopy of ML-60218-treated DLPs shows dose-dependent structural damage. ML-60218-mediated (10 μM ) viroplasm disruption causes NSP5 dephosphorylation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 577784-91-9
Appearance Solid
Molecular Weight 452.38
Formel C₁₉H₁₅Cl₂N₃O₂S₂
Color White to light yellow
SMILES

O=S(C1=CC=CC=C1Cl)(NC2=CC(C3=C(C)C4=CC(Cl)=CC=C4S3)=NN2C)=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (11)

All WBCell Proliferation/Viability AssayRT-PCR

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Journal Impact Factor

Mol Cell

NAT10 promotes cancer metastasis by modulating p300/CBP activity through chromatin-associated tRNA. [Abstract]

2025 Dec 18;85(24):4526-4544.e18. PMID: 41344331

ML-60218 purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Dec 18;85(24):4526-4544.e18. [Abstract]

Western blot showing the level of p300, CBP, H3K27ac and H4K20ac in 4T1 cells treated with ML-60218 (25 μM) for 48 h (left) and 96 h (right). The results showed that after 48 hours of treatment with ML-60218, the levels of p300, CBP, H3K27ac, and H4K20ac increased.

ML-60218 purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Dec 18;85(24):4526-4544.e18. [Abstract]

qRT-PCR analysis showing the levels of RNA polymerase III-transcribed genes in Pol III-inhibitor, ML-60218-treated (25 µM; 48 or 96 h) 4T1 cells at different time point.
Nucleic Acids Res

CRISPuRe-seq: pooled screening of barcoded ribonucleoprotein reporters reveals regulation of RNA polymerase III transcription by the integrated stress response via mTOR. [Abstract]

2025 Feb 8;53(4):gkaf062. PMID: 39921565
Cell Commun Signal

Elevation of effective p53 expression sensitizes wild-type p53 breast cancer cells to CDK7 inhibitor THZ1. [Abstract]

2022 Sep 5;20(1):96. PMID: 36058938

ML-60218 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2022 Sep 5;20(1):96. [Abstract]

CCk8 assay was meant to detect MCF-7 cell viability under Pol III inhibitor ML-60218 (7.5-60 μM； 24-48 h) +/- nutlin-3.
Cell Chem Biol

Signaling of glycoRNAs to Siglec-11 protects neurons by suppressing NF-κB. [Abstract]

2026 Jun 16:S2451-9456(26)00196-0. PMID: 42302779
Cell Rep

Subcellular localization shapes the fate of RNA polymerase III. [Abstract]

2023 Aug 8;42(8):112941. PMID: 37556328
PLoS Pathog

African swine fever virus I267L acts as an important virulence factor by inhibiting RNA polymerase III-RIG-I-mediated innate immunity. [Abstract]

2022 Jan 28;18(1):e1010270. PMID: 35089988

ML-60218 purchased from MedChemExpress. Usage Cited in: PLoS Pathog. 2022 Jan 28;18(1):e1010270. [Abstract]

PK-15 cells or PAMs (1×10⁶) were pre-treated with the indicated concentrations of the RNA Pol-III inhibitor ML-60218 (20-50 μM) for 6 hours before transfection with poly(dA:dT), ASFV-AT, ASFV-GC or poly(I:C) (1 μg/mL) for 4 hours. The mRNA levels of IFNB1 were analyzed by RT-qPCR. The results showed that ML-60218, a inhibitor of RNA Pol‑III, markedly inhibited transcription of the IFNB1 gene induced by transfected poly(dA:dT) and ASFV/DNA‑AT, but not by poly(I:C) or ASFV/DNA‑GC, in porcine PK‑15 cells and PAMs, respectively.
bioRxiv

2026 May 29.
bioRxiv

2026 May 29.
bioRxiv

2026 Jan 7.
bioRxiv

2025 Nov 5.
bioRxiv

Evidence of RNA polymerase III recruitment and transcription at protein-coding gene promoters. [Abstract]

2024 Jun 9:2024.06.08.598009. PMID: 38895345

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (221.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2105 mL

11.0527 mL

22.1053 mL

5 mM

0.4421 mL

2.2105 mL

4.4211 mL

10 mM

0.2211 mL

1.1053 mL

2.2105 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wu L, et al. Novel small-molecule inhibitors of RNA polymerase III. Eukaryot Cell. 2003;2(2):256-264. [Content Brief]

[2]. Yee NS, et al. Targeting developmental regulators of zebrafish exocrine pancreas as a therapeutic approach in human pancreatic cancer. Biol Open. 2012;1(4):295-307. [Content Brief]

[3]. Eichwald C, et al. Identification of a Small Molecule That Compromises the Structural Integrity of Viroplasms and Rotavirus Double-Layered Particles. J Virol. 2018;92(3):e01943-17. Published 2018 Jan 17. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2105 mL	11.0527 mL	22.1053 mL	55.2633 mL
	5 mM	0.4421 mL	2.2105 mL	4.4211 mL	11.0527 mL
	10 mM	0.2211 mL	1.1053 mL	2.2105 mL	5.5263 mL
	15 mM	0.1474 mL	0.7368 mL	1.4737 mL	3.6842 mL
	20 mM	0.1105 mL	0.5526 mL	1.1053 mL	2.7632 mL
	25 mM	0.0884 mL	0.4421 mL	0.8842 mL	2.2105 mL
	30 mM	0.0737 mL	0.3684 mL	0.7368 mL	1.8421 mL
	40 mM	0.0553 mL	0.2763 mL	0.5526 mL	1.3816 mL
	50 mM	0.0442 mL	0.2211 mL	0.4421 mL	1.1053 mL
	60 mM	0.0368 mL	0.1842 mL	0.3684 mL	0.9211 mL
	80 mM	0.0276 mL	0.1382 mL	0.2763 mL	0.6908 mL
	100 mM	0.0221 mL	0.1105 mL	0.2211 mL	0.5526 mL

Keywords

ML-60218

11 Cited Publications

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ML-60218

Anti-Infection Compound Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Heterocyclic Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) ML-60218

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

ML-60218

Anti-Infection Compound Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Heterocyclic Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) ML-60218

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

ML-60218 Related Classifications

HY-122122 Related Classifications