NaPi2b-IN-2
Based on 1 publication(s) in Google Scholar
NaPi2b-IN-2 is a potent inhibitor of sodium-dependent phosphate transporter 2b (SLC34A2, NaPi2b), with an IC50 value of 38 nM against human NaPi2b. NaPi2b-IN-2 reduces intracellular phosphate levels and enhances innate immune gene expression. NaPi2b-IN-2 can be used in research related to hyperphosphatemia.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.02%
- CAS. Nr.: 2227445-31-8
- Formel: C41H47ClF3N5O5S
- Molecular Weight:814.36
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) NaPi2b-IN-2
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Biologische Aktivität
Beschreibung
IC50 & Target
IC50: 38 nM (human NaPi2b)[1]
In Vitro
NaPi2b-IN-2 (Compound 5) potently inhibits human NaPi2b with an IC50 of 38 nM[1].
NaPi2b-IN-2 (100 nM; 40 h) reduces intracellular phosphate levels and enhances diABZI-induced innate immune gene expression, including IFIT2, IFIT3 and CXCL10, in OVCAR-3 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Parmacokinetics
Chemical Information
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CAS. Nr. 2227445-31-8
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Appearance Solid
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Molecular Weight 814.36
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Formel C41H47ClF3N5O5S
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Color Light yellow to yellow
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SMILES
O=C(NC1=C(C2=C(S1)CC(C)(CC2)C)C(C3=CN(C4=CC(C(F)(F)F)=C(Cl)C=C4)N=C3)=O)C5=CC(CN(C(CC)CC)CCN(C)C(CCC(O)=O)=O)=CC=C5
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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mBio
Human-specific activation of the DUX4-SLC34A2 axis by herpesviruses suppresses antiviral innate immunity. [Abstract]2025 Dec 10;16(12):e0255425. PMID: 41211819
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (122.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (3.07 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
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RT-PCR
Reverse transcription technology uses RNA as a template to synthesize DNA. RT-PCR is simple, specific and sensitive, and can be used to detect gene expression levels and expression differences in cells; detect RNA virus content; clone cDNA sequences of specific genes.
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Real Time qPCR (Q-PCR)
Real-time quantitative PCR (qPCR) quantifies an amplifiable nucleic-acid target by monitoring fluorescence during PCR cycling rather than measuring product only after amplification. The increase in fluorescence tracks accumulation of PCR product, and the quantification cycle (Cq; historically also Ct/CP) is related to the initial amount of target: samples containing more starting target generally reach the defined fluorescence threshold in fewer cycles.
Reinheit & Dokumentation
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Data Sheet (276 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2280 mL | 6.1398 mL | 12.2796 mL | 30.6990 mL |
| 5 mM | 0.2456 mL | 1.2280 mL | 2.4559 mL | 6.1398 mL | |
| 10 mM | 0.1228 mL | 0.6140 mL | 1.2280 mL | 3.0699 mL | |
| 15 mM | 0.0819 mL | 0.4093 mL | 0.8186 mL | 2.0466 mL | |
| 20 mM | 0.0614 mL | 0.3070 mL | 0.6140 mL | 1.5349 mL | |
| 25 mM | 0.0491 mL | 0.2456 mL | 0.4912 mL | 1.2280 mL | |
| 30 mM | 0.0409 mL | 0.2047 mL | 0.4093 mL | 1.0233 mL | |
| 40 mM | 0.0307 mL | 0.1535 mL | 0.3070 mL | 0.7675 mL | |
| 50 mM | 0.0246 mL | 0.1228 mL | 0.2456 mL | 0.6140 mL | |
| 60 mM | 0.0205 mL | 0.1023 mL | 0.2047 mL | 0.5116 mL | |
| 80 mM | 0.0153 mL | 0.0767 mL | 0.1535 mL | 0.3837 mL | |
| 100 mM | 0.0123 mL | 0.0614 mL | 0.1228 mL | 0.3070 mL |
NaPi2b-IN-2 Related Classifications
HY-145894 Related Classifications
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