BCL6-IN-4
Based on 1 Customer Validation
BCL6-IN-4 is a potent B-cell lymphoma 6 (BCL6) inhibitor with an IC50 of 97 nM. BCL6-IN-4 has anti-tumor activities.
For research use only. We do not sell to patients.
- Purity: 99.62%
- CAS No.: 2253879-65-9
- Formula: C25H35ClN6O3
- Molecular Weight:503.04
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
IC50: 97 nM (BCL6)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| OCI-Ly1 | DC50 |
10 μM
Compound: 25b
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Induction of BCL6 degradation in human OCI-LY1 cells incubated for 2 hrs by MSD electrochemiluminescent immunoassay
Induction of BCL6 degradation in human OCI-LY1 cells incubated for 2 hrs by MSD electrochemiluminescent immunoassay
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[PMID: 32275432] |
| OCI-Ly1 | GI50 |
2.8 μM
Compound: 25b
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Antiproliferative activity agianst human OCI-LY1 cells assessed as growth inhibition incubated for 14 days by CellTiter Glo reagent based assay
Antiproliferative activity agianst human OCI-LY1 cells assessed as growth inhibition incubated for 14 days by CellTiter Glo reagent based assay
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[PMID: 32275432] |
| OCI-Ly3 | GI50 |
4.2 μM
Compound: 25b
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Antiproliferative activity agianst human OCI-LY3 cells assessed as growth inhibition incubated for 14 days by CellTiter Glo reagent based assay
Antiproliferative activity agianst human OCI-LY3 cells assessed as growth inhibition incubated for 14 days by CellTiter Glo reagent based assay
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[PMID: 32275432] |
BCL6-IN-4 (compound 25b) inhibits OCI-Ly1 and OCI-Ly3 cell lines growth with GI50 values of 2.8 μM and 4.2 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2253879-65-9
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Appearance Solid
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Molecular Weight 503.04
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Formula C25H35ClN6O3
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Color White to light brown
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SMILES
O=C1N(CCC(C)(O)C)C2=CC(NC3=NC(N4CC(C)(C)OC(C)(C)C4)=NC=C3Cl)=CC=C2N1C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 73.33 mg/mL (145.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5.5 mg/mL (10.93 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 5.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (55.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9879 mL | 9.9396 mL | 19.8791 mL | 49.6978 mL |
| 5 mM | 0.3976 mL | 1.9879 mL | 3.9758 mL | 9.9396 mL | |
| 10 mM | 0.1988 mL | 0.9940 mL | 1.9879 mL | 4.9698 mL | |
| 15 mM | 0.1325 mL | 0.6626 mL | 1.3253 mL | 3.3132 mL | |
| 20 mM | 0.0994 mL | 0.4970 mL | 0.9940 mL | 2.4849 mL | |
| 25 mM | 0.0795 mL | 0.3976 mL | 0.7952 mL | 1.9879 mL | |
| 30 mM | 0.0663 mL | 0.3313 mL | 0.6626 mL | 1.6566 mL | |
| 40 mM | 0.0497 mL | 0.2485 mL | 0.4970 mL | 1.2424 mL | |
| 50 mM | 0.0398 mL | 0.1988 mL | 0.3976 mL | 0.9940 mL | |
| 60 mM | 0.0331 mL | 0.1657 mL | 0.3313 mL | 0.8283 mL | |
| 80 mM | 0.0248 mL | 0.1242 mL | 0.2485 mL | 0.6212 mL | |
| 100 mM | 0.0199 mL | 0.0994 mL | 0.1988 mL | 0.4970 mL |
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