- Biochemical Assay Reagents
- Indicators
Indicators

Indicators are used in chemical reactions to to signal the progress or endpoint of a reaction, changing color rapidly upon completion of the reaction. They are commonly employed in acid-base titration, redox titration, and other chemical analyses to ascertain whether the reaction has been completed.
Based on the reaction types, indicators can be divided into acid-base indicators, metal ion indicators, redox indicators, adsorption indicators, fluorescent indicators, etc.
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Indicators (140)
- Formula: C25H30ClN3
- Molecular Weight: 407.98
Crystal violet, IND (0.0-2.0) is a specialized indicator suitable for strongly acidic systems, which reflects changes in pH within the range of 0.0-2.0 through color changes. Crystal violet, IND (0.0-2.0) is widely used in acid-base titration, chemical analysis, and industrial testing fields.
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- Formula: C19H10Br4O5S
- Molecular Weight: 669.96
Bromophenol blue indicator (3.0-4.6) is a synthetic dye commonly used as an acid-base indicator with a transition range of pH 3.0-4.6. Bromophenol blue indicator (3.0-4.6) is water soluble and changes color from yellow to blue as the pH of the solution changes from acidic to basic. Its unique chemical properties make it an important ingredient in a variety of scientific applications, especially in biochemistry and molecular biology. In addition, it can be used as a stain in microbiology and histology. However, Bromophenol blue indicator (3.0-4.6) has potential irritating and staining properties.
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- Formula: C9H13N3
- Molecular Weight: 163.22
1-(2-Pyridyl)piperazine is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
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- Formula: C21H16Br2O5S
- Molecular Weight: 540.22
Bromocresol purple is a pH indicator, whose color changes from yellow to purple when the pH increases from 5.2 to 6.8. Bromocresol purple can be used for detecting yeast cells with plasma membrane damage and measuring serum albumin concentrations.
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- Formula: C20H12N3NaO7S
- Molecular Weight: 461.38
Eriochrome black T, Indicator is a complexing agent for metal ions (e.g., Ca2+, Mg2+) and is used as an indicator in complexometric titrations. Eriochrome black T, Indicator forms colored complexes with metal ions through covalent coordination bonds, and indicates the endpoint of the titration by color change. Eriochrome black T, Indicator can be used as an anionic azo dye in photocatalytic degradation studies to evaluate the performance of photocatalysts. The reaction solution of Eriochrome black T, Indicator combined with Mg2+ is initially purple. During loop-mediated isothermal amplification (LAMP), the color changes from purple to sky blue due to the consumption of Mg2+ by the formation of magnesium pyrophosphate, indicating a positive reaction. The optimal concentration of Eriochrome black T, Indicator in LAMP is 60 μM, and the detection limit for Mycobacterium tuberculosis is 1 pg DNA/reaction.
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- Formula: C12H6Cl2NNaO2.xH2O
2,6-Dichlorophenolindophenol (DCIP; DPIP) sodium hydrate is a redox chromogenic indicator with a redox potential of +217 mV relative to SHE, and it acts as a substrate for reduction reactions. During the metabolic process of nutrient consumption by Saccharomyces cerevisiae, 2,6-Dichlorophenolindophenol sodium hydrate is reduced from dark blue to colorless, resulting in a decrease in absorbance. 2,6-Dichlorophenolindophenol sodium hydrate is widely used in spectrophotometric biochemical oxygen demand determination and preclinical colorimetric toxicity analysis for heavy metal ion detection based on Saccharomyces cerevisiae.
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- Formula: C28H18N3NaO6S2
- Molecular Weight: 579.58
Azocarmine G (Acid Red 101; Rosinduline) is a biochemical assay reagent.
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- Formula: C14H7NaO7S
- Molecular Weight: 342.26
Alizarin red S indicator (C.I. 58005) is a reductively active (quinone-based) anthraquinone dye that forms complexes with metal ions (such as zirconium, calcium) or boric acid to label calcium deposition and perform electrochemical sensing functions. Alizarin Red S undergoes reversible redox reactions (for electrochemical detection) and irreversible chelation (for bone staining). Alizarin Red S is mainly used in bone metabolism research (labeling mineralized tissue), sugar detection (boric acid-sugar competition system) and metal ion sensing (such as zirconium ion detection), and can be used in osteoporosis and metabolic disease research.
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- Formula: C12H18NNaO3S
- Molecular Weight: 279.33
TOPS is a chromogenic substrate. TOPS undergoes an oxidative coupling reaction with 4-aminoantipyrine in the presence of H2O2 and nanocrystalline cobalt selenide. TOPS is used in studies related to uric acid detection.
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- Formula: C34H40Cl2N4O4
- Molecular Weight: 639.61
Mesoporphyrin IX (dihydrochloride) is a photosensitizer that can be used to modify liposomes. Mesoporphyrin IX (dihydrochloride) can insert into lipid vesicles and disrupt the viral membrane structure in vesicular stomatitis virus (VSV), inducing cross-linking of VSV glycoproteins, thereby inhibiting viral activity.
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- Formula: C23H15Cl2Na3O9S
- Molecular Weight: 607.30
Chromeazurol S is a compound belonging to the class of azo dyes. It is often used as an indicator in analytical chemistry to detect metal ions such as copper, nickel, and cobalt. Chromeazurol S turns from yellow to blue in the presence of metal ions, allowing them to be detected and quantified. It can be applied to a test strip or added directly to a solution for analysis.
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- Formula: C28H30N2O3
- Molecular Weight: 442.55
Rhodamine B base (Solvent red 49) is a fluorescent dye (Ex=543 nM; Em=565 nM).
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- Formula: C5H10AgNS2
- Molecular Weight: 256.14
Silver diethyldithiocarbamate (SDDC) is an organic compound consisting of silver ions complexed with the ligand diethyldithiocarbamate. SDDC is mainly used as a reagent in analytical chemistry to detect the presence of copper, iron and other heavy metals in various materials. It acts as a chelating agent, binding to metal ions and forming stable complexes that can be easily analyzed using techniques such as UV-Vis spectroscopy.
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- Formula: C40H32Cl2N8O2
- Molecular Weight: 727.64
Blue Tetrazolium is a tetrazolium salt chromogenic dye that can be reduced to a dark blue formazan product. Blue Tetrazolium is applicable for assays such as succinate dehydrogenase activity detection and reducing sugar quantification. Blue Tetrazolium also serves as a novel corrosion inhibitor for cold-rolled steel in hydrochloric acid solutions. Blue Tetrazolium has a broad wavelength range (480-600 nm), with the maximum absorbance observed at 540 nm.
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- Formula: C15H19BrN2O5
- Molecular Weight: 387.23
Mebrofenin (SQ 26962) is a type of iminodiacetic acid (IDA). Mebrofenin is available as a ready to use the kit for radio-labeling with Tc-99m. Tc-99m Mebrofenin, a diagnostic agent, is used for hepatobiliary imaging. Tc-99m Mebrofenin is the radiopharmaceutical of choice for the evaluation of hepatic function.
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- Formula: NH4Fe(SO4)2·12H2O
- Molecular Weight: 482.19
Ammonium iron(III) sulfate dodecahydrate, 99% is an analytical reagent-grade iron(III) salt and source material for iron(III) reagent or standard solutions in volumetric analysis.
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- Formula: C29H35IN2
- Molecular Weight: 538.51
Cyanine is an excellent fluorescent core scaffold for constructing fluorescent probes. Covalent conjugation of Cyanine with other potent compounds can not only enhance their efficacy but also expand their application modes. Cyanine supports the development of cancer theranostic agents. Cyanine can be used in cancer research.
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- Formula: C13H20NNaO6S
- Molecular Weight: 341.36
DAOS, a Trinder's reagent, is a novel highly water-soluble aniline derivative; are widely used in diagnostic tests and biochemical tests.
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