BML-284 hydrochloride
Based on 41 publication(s) in Google Scholar
BML-284 hydrochloride is a potent and cell-permeable Wnt signaling activator. BML-284 induces TCF-dependent transcriptional activity with an EC50 of 700 nM.
For research use only. We do not sell to patients.
- CAS No.: 2095432-75-8
- Formula: C19H19ClN4O3
- Molecular Weight:386.83
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) BML-284 hydrochloride
More- Sci Adv. 2024 Oct 11;10(41):eadq6795. [Abstract]
- J Hazard Mater. 2023 Apr 15:448:130959. [Abstract]
- Adv Healthc Mater. 2026 Jan 4:e03543. [Abstract]
- Proc Natl Acad Sci U S A. 2021 Jan 12;118(2):e2009539118. [Abstract]
- Free Radic Biol Med. 2025 Nov 21:243:231-244. [Abstract]
- Chemosphere. 2020 Sep;255:126889. [Abstract]
- Sci Total Environ. 2022 Feb 25;809:152102. [Abstract]
- Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]
- Cell Rep. 2024 May 10;43(5):114226. [Abstract]
- Prog Neurobiol. 2024 Jun 28:102654. [Abstract]
- Ecotoxicol Environ Saf. 2021 Nov 27:228:113029. [Abstract]
- Ecotoxicol Environ Saf. 2021 Oct 1:222:112514. [Abstract]
- Biol Direct. 2025 Aug 22;20(1):94. [Abstract]
- Eur J Pharmacol. 2026 Feb 15:1015:178541. [Abstract]
- Comp Biochem Physiol C Toxicol Pharmacol. 2025 Dec 10:301:110432. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2025 Aug 8;1871(8):168013. [Abstract]
- J Cell Physiol. 2023 Mar;238(3):610-630. [Abstract]
- Sci Rep. 2025 Aug 12;15(1):29484. [Abstract]
- Am J Pathol. 2020 Nov;190(11):2237-2250. [Abstract]
- Exp Cell Res. 2026 Apr 1;457(1):114922. [Abstract]
- Food Chem Toxicol. 2025 Oct:204:115660. [Abstract]
- Mol Med Rep. 2024 May;29(5):75. [Abstract]
- Environ Toxicol. 2024 Mar;39(3):1836-1846. [Abstract]
- Mol Carcinog. 2023 Nov;62(11):1686-1699. [Abstract]
- Cell Biol Int. 2026 Feb;50(2):e70136. [Abstract]
- Genomics. 2024 Nov;116(6):110943. [Abstract]
- Discov Oncol. 2024 Dec 18;15(1):803. [Abstract]
- Adv Biol (Weinh). 2025 Mar 24:e2400564. [Abstract]
- Mol Biotechnol. 2024 May 14. [Abstract]
- Exp Ther Med. 2020 Feb;19(2):817-824. [Abstract]
- Biol Pharm Bull. 2024 Feb 22;47(2):486-498. [Abstract]
- Biol Pharm Bull. 2021 Jun 1;44(6):771-779. [Abstract]
- In Vitro Cell Dev Biol Anim. 2019 Apr;55(4):252-259. [Abstract]
- Mol Cell Toxicol. 2024 Oct 31.
- Acta Cir Bras. 2023 Dec 4:38:e385223. [Abstract]
- Egypt J Med Hum Genet. 2025 Dec 22;26(1):189.
- Seoul National University. 2032.
- Research Square Preprint. 2023 May 17.
- Research Square Print. 2023 Mar 13.
- Eur Rev Med Pharmacol Sci. 2020 Nov;24(21):11199-11211. [Abstract]
- ACS Comb Sci. 2019 Dec 9;21(12):805-816. [Abstract]
Customer Validation & Images
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WB
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Cell Imaging/Staining
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RT-PCR
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IF
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In Vivo Efficacy Study
Biological Activity
EC50: 700 nM (wnt signaling)[1]
BML-284 (10 µM; 24 hours) induces the expression of β-catenin significantly when compared with the NC group. It also partially reverses the effects induced by pizotifen on E-cadherin and N-cadherin expression in MNK45 and AGS cells compared with the pizotifen-treated group[1].BML-284 (10 µM; 24 hours) significantly increases the migration and invasion of both MNK45 and AGS cells and partially restores the migratory and invasive abilities of cells inhibited by pizotifen[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human gastric cancer cell lines MNK45 and AGS
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Concentration:10 µM
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Incubation Time:24 hours
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Result:Induced β-catenin expression and reserved E-cadherin and N-cadherin expression in MNK45 and AGS cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2095432-75-8
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Molecular Weight 386.83
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Formula C19H19ClN4O3
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SMILES
NC1=NC(C2=CC=CC(OC)=C2)=CC(NCC3=CC=C(OCO4)C4=C3)=N1.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (41)
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Journal Impact Factor
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Most Recent
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Sci Adv
CLIC5 promotes myoblast differentiation and skeletal muscle regeneration via the BGN-mediated canonical Wnt/β-catenin signaling pathway. [Abstract]2024 Oct 11;10(41):eadq6795. PMID: 39999205 -
J Hazard Mater
Insights into the circadian rhythm alterations of the novel PFOS substitutes F-53B and OBS on adult zebrafish. [Abstract]2023 Apr 15:448:130959. PMID: 36860044 -
Adv Healthc Mater
Bioinspired Microgel Assembly of Liver-Derived Decellularized Extracellular Matrix Enhances Dentin-Pulp Regeneration via Stemness Awakening. [Abstract]2026 Jan 4:e03543. PMID: 41486849 -
Proc Natl Acad Sci U S A
2021 Jan 12;118(2):e2009539118. PMID: 33376206 -
Free Radic Biol Med
Triphenyltin chloride suppresses caudal fin regeneration in zebrafish by inhibiting Wnt signaling. [Abstract]2025 Nov 21:243:231-244. PMID: 41275934 -
Chemosphere
2020 Sep;255:126889. PMID: 32388256 -
Sci Total Environ
Hematopoietic stem cell and immunotoxicity in zebrafish embryos induced by exposure to Metalaxyl-M. [Abstract]2022 Feb 25;809:152102. PMID: 34863748 -
Cell Death Discov
Suppressed Akt/GSK-3β/β-catenin signaling contributes to excessive adipogenesis of fibro-adipogenic progenitors after rotator cuff tears. [Abstract]2023 Aug 25;9(1):312. PMID: 37626040
BML-284 hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]
Protein level of β-catenin, PPARγ, and GAPDH for RCT-FAPs (FAPs isolated from supraspinatus muscle one week after tendon tears) with or without β-catenin agonist BML-284 (1 μM) treatment.
BML-284 hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]
The oil red staining and statistical analysis of RCT-FAPs after 7-day adipogenic induction in AIM with DMSO or BML-284 (1 μM).
BML-284 hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]
Relative mRNA expression level of adipogenic genes for RCT-FAPs after 7-day adipogenic induction in AIM with DMSO or BML-284 (1 μM).
BML-284 hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]
Immunofluorescence staining of perilipin1 for supraspinatus muscles from the DMSO group and BML-284 (1 μM) group.
BML-284 hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]
Gross appearance of supraspinatus muscles from the DMSO and BML-284 (1 μM) treatment groups.
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Cell Rep
Astrocyte-derived CHI3L1 signaling impairs neurogenesis and cognition in the demyelinated hippocampus. [Abstract]2024 May 10;43(5):114226. PMID: 38733586 -
Prog Neurobiol
Astrocyte-secreted C3 signaling impairs neuronal development and cognition in autoimmune diseases. [Abstract]2024 Jun 28:102654. PMID: 38945516 -
Ecotoxicol Environ Saf
2021 Nov 27:228:113029. PMID: 34847436 -
Ecotoxicol Environ Saf
2021 Oct 1:222:112514. PMID: 34280841 -
Biol Direct
Inhibition of PRC1 elicits immunogenic cell death by triggering ROS-dependent ER stress in colorectal cancer via the Wnt/β-catenin signaling pathway. [Abstract]2025 Aug 22;20(1):94. PMID: 40847353 -
Eur J Pharmacol
Melatonin suppresses cellular senescence in diabetic kidney disease though the Wnt/β-catenin signaling pathway. [Abstract]2026 Feb 15:1015:178541. PMID: 41534557 -
Comp Biochem Physiol C Toxicol Pharmacol
2-Hydroxyanthraquinone exposure causes the damage of cerebrovascular and blood brain barrier in zebrafish via inducing inflammation and downregulation of the Wnt/β-catenin signaling pathway. [Abstract]2025 Dec 10:301:110432. PMID: 41386556 -
Biochim Biophys Acta Mol Basis Dis
Glioma cells produce soluble PD-L1 via the Wnt/β-catenin signaling pathway to suppress CD8+ T cell activity. [Abstract]2025 Aug 8;1871(8):168013. PMID: 40784599 -
J Cell Physiol
Identification of key events and regulatory networks in the formation process of primordial germ cell based on proteomics. [Abstract]2023 Mar;238(3):610-630. PMID: 36745473 -
Sci Rep
Cardamonin inhibits the growth and stemness of osteosarcoma stem cells by inducing autophagy and inhibiting the Wnt/β-catenin signaling pathway. [Abstract]2025 Aug 12;15(1):29484. PMID: 40796594 -
Am J Pathol
Insulin Promotes Corneal Nerve Repair and Wound Healing in Type 1 Diabetic Mice by Enhancing Wnt/β-Catenin Signaling. [Abstract]2020 Nov;190(11):2237-2250. PMID: 32858016 -
Exp Cell Res
NOP14 promotes proliferative diabetic retinopathy through ribosome biogenesis and endothelial dysfunction via Wnt/β-catenin signaling activation. [Abstract]2026 Apr 1;457(1):114922. PMID: 41638389 -
Food Chem Toxicol
2025 Oct:204:115660. PMID: 40706976 -
Mol Med Rep
Pigment epithelium‑derived factor inhibits proliferation, invasion and angiogenesis, and induces ferroptosis of extravillous trophoblasts by targeting Wnt‑β‑catenin/VEGF signaling in placenta accreta spectrum. [Abstract]2024 May;29(5):75. PMID: 38488028 -
Environ Toxicol
Tescalcin modulates bone marrow-derived mesenchymal stem cells osteogenic differentiation via the Wnt/β-catenin signaling pathway. [Abstract]2024 Mar;39(3):1836-1846. PMID: 38124301 -
Mol Carcinog
TRIM16 E121D variant affects the risk and prognosis of hepatocellular carcinoma by modulating the Wnt/β-catenin pathway. [Abstract]2023 Nov;62(11):1686-1699. PMID: 37477507 -
Cell Biol Int
RFC4 Promotes the Metastasis of Colorectal Cancer by Regulating the Wnt/β-Catenin Pathway. [Abstract]2026 Feb;50(2):e70136. PMID: 41574400 -
Genomics
PLOD1 promote proliferation and migration with glycolysis via the Wnt/β-catenin pathway in THCA. [Abstract]2024 Nov;116(6):110943. PMID: 39424162 -
Discov Oncol
PTEN inhibits epithelial mesenchymal transition of thyroid cancer cells by regulating the Wnt/β-Catenin signaling pathway. [Abstract]2024 Dec 18;15(1):803. PMID: 39692895 -
Adv Biol (Weinh)
IER3 Facilitates Tumor Progression and Aberrant Glycolysis via Activating wnt/β-Catenin Pathway in Oral Squamous Cell Carcinoma. [Abstract]2025 Mar 24:e2400564. PMID: 40128149 -
Mol Biotechnol
Nucleoporin 93 Regulates Cancer Cell Growth and Stemness in Bladder Cancer via Wnt/β-Catenin Signaling. [Abstract]2024 May 14. PMID: 38744786 -
Exp Ther Med
2020 Feb;19(2):817-824. PMID: 32010241 -
Biol Pharm Bull
Loureirin A Promotes Cell Differentiation and Suppresses Migration and Invasion of Melanoma Cells via WNT and AKT/mTOR signaling Pathways. [Abstract]2024 Feb 22;47(2):486-498. PMID: 38199251 -
Biol Pharm Bull
2021 Jun 1;44(6):771-779. PMID: 33731543 -
In Vitro Cell Dev Biol Anim
A1CF-Axin2 signal axis regulates apoptosis and migration in Wilms tumor-derived cells through Wnt/β-catenin pathway. [Abstract]2019 Apr;55(4):252-259. PMID: 30825095 -
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Acta Cir Bras
miR-29c-3p represses the angiogenesis of esophageal squamous cell carcinoma by targeting SERPINH1 to regulate the Wnt signaling pathway. [Abstract]2023 Dec 4:38:e385223. PMID: 38055382 -
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Eur Rev Med Pharmacol Sci
MSCs reduce airway remodeling in the lungs of asthmatic rats through the Wnt/β-catenin signaling pathway. [Abstract]2020 Nov;24(21):11199-11211. PMID: 33215438 -
ACS Comb Sci
Benzimidazolyl-pyrazolo[3,4- b]pyridinones, Selective Inhibitors of MOLT-4 Leukemia Cell Growth and Sea Urchin Embryo Spiculogenesis: Target Quest. [Abstract]2019 Dec 9;21(12):805-816. PMID: 31689077
Purity & Documentation
References
[1]. Liu J, et al. A small-molecule agonist of the Wnt signaling pathway. Angew Chem Int Ed Engl. 2005 Mar 18;44(13):1987-90. [Content Brief]
[2]. Ying Jiang, et al. Pizotifen inhibits the proliferation and invasion of gastric cancer cells. Exp Ther Med. 2020 Feb;19(2):817-824. [Content Brief]
[3]. Yunlong Meng, et al. Exposure to pyrimethanil induces developmental toxicity and cardiotoxicity in zebrafish. Chemosphere. 2020 Sep;255:126889 [Content Brief]
Calculators
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