1. Stem Cell/Wnt
  2. Wnt
  3. BML-284

BML-284 

Cat. No.: HY-19987 Purity: 99.98%
Handling Instructions

BML-284 is a potent and cell-permeable Wnt signaling activator. BML-284 induces TCF-dependent transcriptional activity with an EC50 of 700 nM.

For research use only. We do not sell to patients.

BML-284 Chemical Structure

BML-284 Chemical Structure

CAS No. : 853220-52-7

Size Price Stock Quantity
10 mM * 1  mL in DMSO USD 77 In-stock
Estimated Time of Arrival: December 31
5 mg USD 70 In-stock
Estimated Time of Arrival: December 31
10 mg USD 100 In-stock
Estimated Time of Arrival: December 31
50 mg USD 350 In-stock
Estimated Time of Arrival: December 31
100 mg USD 550 In-stock
Estimated Time of Arrival: December 31
200 mg USD 810 In-stock
Estimated Time of Arrival: December 31
500 mg   Get quote  
1 g   Get quote  

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Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BML-284 is a potent and cell-permeable Wnt signaling activator. BML-284 induces TCF-dependent transcriptional activity with an EC50 of 700 nM[1].

IC50 & Target

EC50: 700 nM (TCF-dependent transcriptional activity)[1]

In Vitro

BML-284 (10 µM; 24 hours) significantly increases the migration and invasion of both MNK45 and AGS cells and partially restores the migratory and invasive abilities of cells inhibited by pizotifen[1].
BML-284 (10 µM; 24 hours) induces the expression of β-catenin significantly when compared with the NC group. It also partially reverses the effects induced by pizotifen on E-cadherin and N-cadherin expression in MNK45 and AGS cells compared with the pizotifen-treated group[1].

Western Blot Analysis[2]

Cell Line: Human gastric cancer cell lines MNK45 and AGS[1]
Concentration: 10 µM
Incubation Time: 24 hours
Result: Induced β-catenin expression and reserved E-cadherin and N-cadherin expression in MNK45 and AGS cells.
In Vivo

BML-284 (10 ng) combines with Pyrimethanil (4 mg/L) could partially rescue the malformed phenotype and cardiac defects induced by Pyrimethanil in Tg (myl7:EGFP) transgenic embryos at 5.5 hpf are transferred into plates with 20 embryos[1].

Molecular Weight

350.37

Formula

C₁₉H₁₈N₄O₃

CAS No.

853220-52-7

SMILES

NC1=NC(C2=CC=CC(OC)=C2)=CC(NCC3=CC=C(OCO4)C4=C3)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (285.41 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8541 mL 14.2706 mL 28.5413 mL
5 mM 0.5708 mL 2.8541 mL 5.7083 mL
10 mM 0.2854 mL 1.4271 mL 2.8541 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (7.14 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (7.14 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (7.14 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 99.98%

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Keywords:

BML-284BML284BML 284WntTCFMNK45AGS E-cadherinN-cadherinmalformed phenotypecardiac defectInhibitorinhibitorinhibit

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Product Name:
BML-284
Cat. No.:
HY-19987
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