BML-284
Based on 41 publication(s) in Google Scholar
BML-284 is a potent and cell-permeable Wnt signaling activator. BML-284 induces TCF-dependent transcriptional activity with an EC50 of 700 nM.
For research use only. We do not sell to patients.
- Purity: 99.99%
- CAS No.: 853220-52-7
- Formula: C19H18N4O3
- Molecular Weight:350.37
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Stem Cell Signaling Compound Library
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Publications Citing Use of MedChemExpress (MCE) BML-284
More- Sci Adv. 2024 Oct 11;10(41):eadq6795. [Abstract]
- J Hazard Mater. 2023 Apr 15:448:130959. [Abstract]
- Adv Healthc Mater. 2026 Jan 4:e03543. [Abstract]
- Proc Natl Acad Sci U S A. 2021 Jan 12;118(2):e2009539118. [Abstract]
- Free Radic Biol Med. 2025 Nov 21:243:231-244. [Abstract]
- Chemosphere. 2020 Sep;255:126889. [Abstract]
- Sci Total Environ. 2022 Feb 25;809:152102. [Abstract]
- Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]
- Cell Rep. 2024 May 10;43(5):114226. [Abstract]
- Prog Neurobiol. 2024 Jun 28:102654. [Abstract]
- Ecotoxicol Environ Saf. 2021 Nov 27:228:113029. [Abstract]
- Ecotoxicol Environ Saf. 2021 Oct 1:222:112514. [Abstract]
- Biol Direct. 2025 Aug 22;20(1):94. [Abstract]
- Eur J Pharmacol. 2026 Feb 15:1015:178541. [Abstract]
- Comp Biochem Physiol C Toxicol Pharmacol. 2025 Dec 10:301:110432. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2025 Aug 8;1871(8):168013. [Abstract]
- J Cell Physiol. 2023 Mar;238(3):610-630. [Abstract]
- Sci Rep. 2025 Aug 12;15(1):29484. [Abstract]
- Am J Pathol. 2020 Nov;190(11):2237-2250. [Abstract]
- Exp Cell Res. 2026 Apr 1;457(1):114922. [Abstract]
- Food Chem Toxicol. 2025 Oct:204:115660. [Abstract]
- Mol Med Rep. 2024 May;29(5):75. [Abstract]
- Environ Toxicol. 2024 Mar;39(3):1836-1846. [Abstract]
- Mol Carcinog. 2023 Nov;62(11):1686-1699. [Abstract]
- Cell Biol Int. 2026 Feb;50(2):e70136. [Abstract]
- Genomics. 2024 Nov;116(6):110943. [Abstract]
- Discov Oncol. 2024 Dec 18;15(1):803. [Abstract]
- Adv Biol (Weinh). 2025 Mar 24:e2400564. [Abstract]
- Mol Biotechnol. 2024 May 14. [Abstract]
- Exp Ther Med. 2020 Feb;19(2):817-824. [Abstract]
- Biol Pharm Bull. 2024 Feb 22;47(2):486-498. [Abstract]
- Biol Pharm Bull. 2021 Jun 1;44(6):771-779. [Abstract]
- In Vitro Cell Dev Biol Anim. 2019 Apr;55(4):252-259. [Abstract]
- Mol Cell Toxicol. 2024 Oct 31.
- Acta Cir Bras. 2023 Dec 4:38:e385223. [Abstract]
- Egypt J Med Hum Genet. 2025 Dec 22;26(1):189.
- Seoul National University. 2032.
- Research Square Preprint. 2023 May 17.
- Research Square Print. 2023 Mar 13.
- Eur Rev Med Pharmacol Sci. 2020 Nov;24(21):11199-11211. [Abstract]
- ACS Comb Sci. 2019 Dec 9;21(12):805-816. [Abstract]
Customer Validation & Images
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WB
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Cell Imaging/Staining
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RT-PCR
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IF
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In Vivo Efficacy Study
Biological Activity
EC50: 700 nM (TCF-dependent transcriptional activity)[1]
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Cell Line
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Type | Value | Description | References |
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| HCT-116 | IC50 |
0.52 μM
Compound: BML284
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Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability measured after 72 hrs
Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability measured after 72 hrs
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[PMID: 38430854] |
| HeLa | IC50 |
0.09 μM
Compound: BML284
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Antiproliferative activity against human HeLa cells assessed as reduction in cell viability measured after 72 hrs
Antiproliferative activity against human HeLa cells assessed as reduction in cell viability measured after 72 hrs
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[PMID: 38430854] |
| K562 | IC50 |
0.1 μM
Compound: BML284
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Antiproliferative activity against human K562 cells assessed as reduction in cell viability measured after 72 hrs
Antiproliferative activity against human K562 cells assessed as reduction in cell viability measured after 72 hrs
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[PMID: 38430854] |
| MCF7 | IC50 |
0.69 μM
Compound: BML284
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Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs
Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs
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[PMID: 38430854] |
| MOLM-13 | IC50 |
0.19 μM
Compound: BML284
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Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability measured after 72 hrs
Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability measured after 72 hrs
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[PMID: 38430854] |
BML-284 (10 μM; 24 hours) significantly increases the migration and invasion of both MNK45 and AGS cells and partially restores the migratory and invasive abilities of cells inhibited by pizotifen (HY-B0115)[1].BML-284 (10 μM; 24 hours) induces the expression of β-catenin significantly when compared with the NC group. It also partially reverses the effects induced by pizotifen on E-cadherin and N-cadherin expression in MNK45 and AGS cells compared with the pizotifen-treated group[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human gastric cancer cell lines MNK45 and AGS[1]
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Concentration:10 µM
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Incubation Time:24 hours
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Result:Induced β-catenin expression and reserved E-cadherin and N-cadherin expression in MNK45 and AGS cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 853220-52-7
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Appearance Solid
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Molecular Weight 350.37
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Formula C19H18N4O3
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Color White to yellow
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SMILES
NC1=NC(C2=CC=CC(OC)=C2)=CC(NCC3=CC=C(OCO4)C4=C3)=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (41)
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Journal Impact Factor
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Most Recent
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Sci Adv
CLIC5 promotes myoblast differentiation and skeletal muscle regeneration via the BGN-mediated canonical Wnt/β-catenin signaling pathway. [Abstract]2024 Oct 11;10(41):eadq6795. PMID: 39999205 -
J Hazard Mater
Insights into the circadian rhythm alterations of the novel PFOS substitutes F-53B and OBS on adult zebrafish. [Abstract]2023 Apr 15:448:130959. PMID: 36860044 -
Adv Healthc Mater
Bioinspired Microgel Assembly of Liver-Derived Decellularized Extracellular Matrix Enhances Dentin-Pulp Regeneration via Stemness Awakening. [Abstract]2026 Jan 4:e03543. PMID: 41486849 -
Proc Natl Acad Sci U S A
2021 Jan 12;118(2):e2009539118. PMID: 33376206 -
Free Radic Biol Med
Triphenyltin chloride suppresses caudal fin regeneration in zebrafish by inhibiting Wnt signaling. [Abstract]2025 Nov 21:243:231-244. PMID: 41275934 -
Chemosphere
2020 Sep;255:126889. PMID: 32388256 -
Sci Total Environ
Hematopoietic stem cell and immunotoxicity in zebrafish embryos induced by exposure to Metalaxyl-M. [Abstract]2022 Feb 25;809:152102. PMID: 34863748 -
Cell Death Discov
Suppressed Akt/GSK-3β/β-catenin signaling contributes to excessive adipogenesis of fibro-adipogenic progenitors after rotator cuff tears. [Abstract]2023 Aug 25;9(1):312. PMID: 37626040
BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]
Protein level of β-catenin, PPARγ, and GAPDH for RCT-FAPs (FAPs isolated from supraspinatus muscle one week after tendon tears) with or without β-catenin agonist BML-284 (1 μM) treatment.
BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]
The oil red staining and statistical analysis of RCT-FAPs after 7-day adipogenic induction in AIM with DMSO or BML-284 (1 μM).
BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]
Relative mRNA expression level of adipogenic genes for RCT-FAPs after 7-day adipogenic induction in AIM with DMSO or BML-284 (1 μM).
BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]
Immunofluorescence staining of perilipin1 for supraspinatus muscles from the DMSO group and BML-284 (1 μM) group.
BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]
Gross appearance of supraspinatus muscles from the DMSO and BML-284 (1 μM) treatment groups.
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Cell Rep
Astrocyte-derived CHI3L1 signaling impairs neurogenesis and cognition in the demyelinated hippocampus. [Abstract]2024 May 10;43(5):114226. PMID: 38733586 -
Prog Neurobiol
Astrocyte-secreted C3 signaling impairs neuronal development and cognition in autoimmune diseases. [Abstract]2024 Jun 28:102654. PMID: 38945516 -
Ecotoxicol Environ Saf
2021 Nov 27:228:113029. PMID: 34847436 -
Ecotoxicol Environ Saf
2021 Oct 1:222:112514. PMID: 34280841 -
Biol Direct
Inhibition of PRC1 elicits immunogenic cell death by triggering ROS-dependent ER stress in colorectal cancer via the Wnt/β-catenin signaling pathway. [Abstract]2025 Aug 22;20(1):94. PMID: 40847353 -
Eur J Pharmacol
Melatonin suppresses cellular senescence in diabetic kidney disease though the Wnt/β-catenin signaling pathway. [Abstract]2026 Feb 15:1015:178541. PMID: 41534557 -
Comp Biochem Physiol C Toxicol Pharmacol
2-Hydroxyanthraquinone exposure causes the damage of cerebrovascular and blood brain barrier in zebrafish via inducing inflammation and downregulation of the Wnt/β-catenin signaling pathway. [Abstract]2025 Dec 10:301:110432. PMID: 41386556 -
Biochim Biophys Acta Mol Basis Dis
Glioma cells produce soluble PD-L1 via the Wnt/β-catenin signaling pathway to suppress CD8+ T cell activity. [Abstract]2025 Aug 8;1871(8):168013. PMID: 40784599 -
J Cell Physiol
Identification of key events and regulatory networks in the formation process of primordial germ cell based on proteomics. [Abstract]2023 Mar;238(3):610-630. PMID: 36745473 -
Sci Rep
Cardamonin inhibits the growth and stemness of osteosarcoma stem cells by inducing autophagy and inhibiting the Wnt/β-catenin signaling pathway. [Abstract]2025 Aug 12;15(1):29484. PMID: 40796594 -
Am J Pathol
Insulin Promotes Corneal Nerve Repair and Wound Healing in Type 1 Diabetic Mice by Enhancing Wnt/β-Catenin Signaling. [Abstract]2020 Nov;190(11):2237-2250. PMID: 32858016 -
Exp Cell Res
NOP14 promotes proliferative diabetic retinopathy through ribosome biogenesis and endothelial dysfunction via Wnt/β-catenin signaling activation. [Abstract]2026 Apr 1;457(1):114922. PMID: 41638389 -
Food Chem Toxicol
2025 Oct:204:115660. PMID: 40706976 -
Mol Med Rep
Pigment epithelium‑derived factor inhibits proliferation, invasion and angiogenesis, and induces ferroptosis of extravillous trophoblasts by targeting Wnt‑β‑catenin/VEGF signaling in placenta accreta spectrum. [Abstract]2024 May;29(5):75. PMID: 38488028 -
Environ Toxicol
Tescalcin modulates bone marrow-derived mesenchymal stem cells osteogenic differentiation via the Wnt/β-catenin signaling pathway. [Abstract]2024 Mar;39(3):1836-1846. PMID: 38124301 -
Mol Carcinog
TRIM16 E121D variant affects the risk and prognosis of hepatocellular carcinoma by modulating the Wnt/β-catenin pathway. [Abstract]2023 Nov;62(11):1686-1699. PMID: 37477507 -
Cell Biol Int
RFC4 Promotes the Metastasis of Colorectal Cancer by Regulating the Wnt/β-Catenin Pathway. [Abstract]2026 Feb;50(2):e70136. PMID: 41574400 -
Genomics
PLOD1 promote proliferation and migration with glycolysis via the Wnt/β-catenin pathway in THCA. [Abstract]2024 Nov;116(6):110943. PMID: 39424162 -
Discov Oncol
PTEN inhibits epithelial mesenchymal transition of thyroid cancer cells by regulating the Wnt/β-Catenin signaling pathway. [Abstract]2024 Dec 18;15(1):803. PMID: 39692895 -
Adv Biol (Weinh)
IER3 Facilitates Tumor Progression and Aberrant Glycolysis via Activating wnt/β-Catenin Pathway in Oral Squamous Cell Carcinoma. [Abstract]2025 Mar 24:e2400564. PMID: 40128149 -
Mol Biotechnol
Nucleoporin 93 Regulates Cancer Cell Growth and Stemness in Bladder Cancer via Wnt/β-Catenin Signaling. [Abstract]2024 May 14. PMID: 38744786 -
Exp Ther Med
2020 Feb;19(2):817-824. PMID: 32010241 -
Biol Pharm Bull
Loureirin A Promotes Cell Differentiation and Suppresses Migration and Invasion of Melanoma Cells via WNT and AKT/mTOR signaling Pathways. [Abstract]2024 Feb 22;47(2):486-498. PMID: 38199251 -
Biol Pharm Bull
2021 Jun 1;44(6):771-779. PMID: 33731543 -
In Vitro Cell Dev Biol Anim
A1CF-Axin2 signal axis regulates apoptosis and migration in Wilms tumor-derived cells through Wnt/β-catenin pathway. [Abstract]2019 Apr;55(4):252-259. PMID: 30825095 -
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Acta Cir Bras
miR-29c-3p represses the angiogenesis of esophageal squamous cell carcinoma by targeting SERPINH1 to regulate the Wnt signaling pathway. [Abstract]2023 Dec 4:38:e385223. PMID: 38055382 -
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Eur Rev Med Pharmacol Sci
MSCs reduce airway remodeling in the lungs of asthmatic rats through the Wnt/β-catenin signaling pathway. [Abstract]2020 Nov;24(21):11199-11211. PMID: 33215438 -
ACS Comb Sci
Benzimidazolyl-pyrazolo[3,4- b]pyridinones, Selective Inhibitors of MOLT-4 Leukemia Cell Growth and Sea Urchin Embryo Spiculogenesis: Target Quest. [Abstract]2019 Dec 9;21(12):805-816. PMID: 31689077
Solvent & Solubility
DMSO : ≥ 100 mg/mL (285.41 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Liu J, et al. A small-molecule agonist of the Wnt signaling pathway. Angew Chem Int Ed Engl. 2005 Mar 18;44(13):1987-90. [Content Brief]
[2]. Ying Jiang, et al. Pizotifen inhibits the proliferation and invasion of gastric cancer cells. Exp Ther Med. 2020 Feb;19(2):817-824. [Content Brief]
[3]. Yunlong Meng, et al. Exposure to pyrimethanil induces developmental toxicity and cardiotoxicity in zebrafish. Chemosphere. 2020 Sep;255:126889. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8541 mL | 14.2706 mL | 28.5413 mL | 71.3531 mL |
| 5 mM | 0.5708 mL | 2.8541 mL | 5.7083 mL | 14.2706 mL | |
| 10 mM | 0.2854 mL | 1.4271 mL | 2.8541 mL | 7.1353 mL | |
| 15 mM | 0.1903 mL | 0.9514 mL | 1.9028 mL | 4.7569 mL | |
| 20 mM | 0.1427 mL | 0.7135 mL | 1.4271 mL | 3.5677 mL | |
| 25 mM | 0.1142 mL | 0.5708 mL | 1.1417 mL | 2.8541 mL | |
| 30 mM | 0.0951 mL | 0.4757 mL | 0.9514 mL | 2.3784 mL | |
| 40 mM | 0.0714 mL | 0.3568 mL | 0.7135 mL | 1.7838 mL | |
| 50 mM | 0.0571 mL | 0.2854 mL | 0.5708 mL | 1.4271 mL | |
| 60 mM | 0.0476 mL | 0.2378 mL | 0.4757 mL | 1.1892 mL | |
| 80 mM | 0.0357 mL | 0.1784 mL | 0.3568 mL | 0.8919 mL | |
| 100 mM | 0.0285 mL | 0.1427 mL | 0.2854 mL | 0.7135 mL |
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